The Quarterly Journal ofMicroscopical Science

FOUNDED I853

June 1959

VOL. 1 0 0 • PART 2 (THIRD SERIES, NO. 50)

Joint Editors

C. F. A. PA NT IN, ScD., F.R.S.

JOHN R. BAKER, D.Sc, F.R.S.

Published for the Company of Biologists Limited

OXFORD: AT THE CLARENDON PRESS

Price 30J. net Subscription for Volume £4. 45.

THE QUARTERLY JOURNAL OF MICROSCOPICALSCIENCE

NOTICE TO CONTRIBUTORSStudy of this notice may accelerate publication by three months.

Papers offered to the Journal should be sent to Dr. C. F. A. Pan tin, Dept. of Zoology,Downing St., Cambridge. If any preliminary communication on the subject of a paperoffered to the Editors has been sent to another journal, a note to this effect must accompanythe manuscript.

All papers must be typewritten (double-spaced). They must be fully corrected.Authors will be charged for excessive corrections in proof.

Papers should be as concise as possible. Extensive introductory matter and long dis-cussions, commonly required in theses for university degrees, should not be included inthe manuscript. Division of the matter by sub-titles must follow a consistent plan.Excessive subdivision] must be avoided. Not more than three grades of sub-titles willbe printed.

All manuscripts must be accompanied by a summary, in no case exceeding 500 words.The summary should contain no illustration or table and should be intelligible by itselfwithout reference to illustrations or tables in the body of the paper. The summary mustbe a brief statement of the chief results obtained: it must not be a mere list of subjectsdiscussed. It must be placed at the beginning of the paper, after the author's name andpostal address.

Footnotes must not be used. Acknowledgements must be placed at the end of thepaper, before the references.

References must be given in the following style:

GOODRICH, E. S., 1919. The evolution of living organisms. London (Jack).

LANKESTER, E. R., 1904. Quart. J. micr. Sci., 48, 165.or LANKESTER, E. R., 1904. 'The structure and classification of the Arachnida.' Quart.

J. micr. Sci., 48, 165.The list of references must be double-spaced.

ILLUSTRATIONSAll figures, both text-figures and plates, must be numbered in a single series (figs. 1, 2,

3, &c). There must not be one numbered series of text-figures and another separatelynumbered series of plates. The plates should be arranged in their most appropriatepositions in relation to the other illustrations.

No figure should be sent that will not fit easily into an envelope 40x30 cm (about16 x 12 inches).

The magnification of every figure must be indicated by a scale drawn on it.

The lettering of all figures should be clearly written on tracing paper bearing accurateoutlines of the objects indicated by the lettering. Abbreviations should not be used ifthere is room for whole words, but it is often desirable to use them on plates. If used,they must be written without punctuation. Thus, avc might stand for anterior vena cava.

A typewritten (double-spaced) list of the legends of all figures must be provided.Each legend must be complete in itself. Remarks applying to more than one figure willnot be printed.

Text-figures. Line-and-dot drawings in black process ink are preferred. If severaldrawings or graphs compose a single text-figure, they must be labelled A, B, C, &c.All such drawings or graphs, constituting a single text-figure, must be mounted in theircorrect positions on a single sheet of cardboard. Text-figures should be made abouthalf as large again as the final size, in linear dimensions. Thus a text-figure destined tooccupy a whole page should be about 30 x 19J cm.

Plates. When several photomicrographs are grouped to form a single plate, theyshould, if possible, be arranged to occupy an area of approximately the right shape tofill the available space (20 x 13 cm), and they must be neatly pasted to a single piece ofcardboard. The photomicrographs in a single plate must be labelled A, B, C, &c.

Figures should be referred to in the text in accordance with these examples: (fig. 3),(fig. 3, D), (fig. 5, D-F), (fig . 3, D; 5, D-F).

THERE'S NOTHING LIKE THE WATSON 'SERVICEThe most widely used micro-scope. The instrument whichgrows to meet the demands

made upon it.

For the student it is a micro-scope of admirable simplicityand highest quality. As workbecomes more complex the ad-dition of accessories fits the'Service'forthemostspecialized

tasks.

Robust—built for a lifetime ofservice—this microscope hasall the optical and mechanicalrefinements which have madeWATSON STANDARD the

highest praise.

W. WATSON X, SONS LTD.BARNET, HERTS.

AUTOMATICROTARY MICROTOME

A robust and efficient

machine at very moderate

cost. Redesigned with up-to-

date features, for rapid pro-

duction of sections from 1 ft

upwards.

Please write for our newgeneral catalogue

R. & J. BECK LTD.69/71 MORTIMER STREET, LONDON, W.I

[i front]

BOOKS

NATURAL HISTORY

BIOLOGYNew, Second-hand, Old and Rare

CATALOGUES ON REQUEST

WHELDON & WESLEYLTD.

LYTTON LODGE, CODICOTENEAR HITCHIN, HERTS.

Phona: Codlcote 370

HEFFERS

BOOKS ON

BIOLOGY AND

OTHER SCIENCES

ENGLISH

& FOREIGN

W.HEFFER& Sons, Ltd.Petty Cury . Cambridge

You will obtain amazingresults

by cleaning your lenses with Green's

Lens Tissue No. 105. Stocked in books

10x8 cm. also in boxes 20x30 cm.

and in reams of sheets 53.5x42 cm.

Write for price list 25 G/T

J. BARCHAM GREENLIMITED

MAIDSTONE- ENGLAND

MICROPROJECTORS

Education aiIndustry

MICROSCOPESand all

ACCESSORIES

COLLECTINGAPPARATUS

DISSECTINGINSTRUMENTS

SPECIMENSfor

Dissection

STAINS & REAGENTSfor Microscopy

as used and tested in our own Microscope Slide Dept.

See Catalogue S. 6

FLATTERS & GARNETT Ltd.Microscopists and Scientific Instrument Makers

309 OXFORD ROAD, MANCHESTER 13

MICRO8COPI0ALPREPARATIONS

Over 30,000now In stock

LANTERN 8LIDES

and customers'originals

LABORATORYEQUIPMENT

Scientific Books

Catalogues

Application

Over 55 yearservice to

JAMES SWIFT & SON LTD.1 1 3 - 1 1 5 A C A M B E R W E L L R O A D , L O N D O N , S.E. 5

5 W I P I MODEL PMULTI-PURPOSE

MICROSCOPEA fine microscope which can be supplied with any equipmentfavoured by the research microscopist, but which incorporateselegant polarisation facilities.

The analytical value of polarised light has long been utilised bychemist, pharmacist, biologist and mineralogist alike, but here wewish to stress the ability of simply-inserted accessories to revealinformation whose value may be greater because unsuspected. Thepolarising elements also provide a simple means of light control,without any modification of the critical set-up, and inexpensive waveplates can be inserted not only for diagnosis, but to provide brilliantcolour contrast.

The advantage of the polarising attachments in this comprehensiveand versatile microscope far outweigh its small extra cost. Time isprecious and every research microscopist should ensure that theseinformative devices are at his finger-tips, and in an instrument thatwill extract their greatest value.

The potentialities of our Model f are described inSections 6, D, £ and F of our general catalogue.

RODney 5441

Official repairing agents

in Great Britain for

CARL ZEISS, GERMANY, and BAUSCH & LOMB, U.S.A.

SPECIALISTSIN

MICROSCOPE REPAIRSALL BEST MAKES

We manufacture replacement spares, if practicable

COOK & PERKINS LTDPembroke Works, Pembroke Road, Muswell Hill, London, N. 10

Telephone: TUDor 9731-2-3

JOURNAL OF THE

Marine Biological AssociationOF THE UNITED KINGDOM

THE ASSOCIATION was founded in 1884 to promote accurate researchesleading to the advancement of zoological and botanical science and to anincrease in our knowledge of the food, life, conditions, and habits ofBritish fishes.' The work of the Association is carried out at their labora-tory at Plymouth, where a rich and varied fauna is to be found.

The journal contains records of the work done at the laboratory, togetherwith articles on original research in marine biology, physiology, and alliedsubjects from other research establishments in the British Isles andoverseas.

The journal is issued three times a year, the price of each part varies withthe amount of material contained. Orders may be placed with yourbookseller or sent direct to the publishers:

Current issue, Vol. 38, No. 2. June 1959

Cambridge University PressBENTLEY HOUSE : 200 EUSTON ROAD : LONDON, N.W. 1

[4]

THE PRIOR MICRO-MANIPULATOR

This instrument is designedto meet the needs of re-search workers carryingout fine movements underthe Low-power Microscopewith particular applicationin Physiology, Biophysics,and Genetics, &c. It con-sists of a vertical movementwith fine and coarse adjust-ment supporting a headwith two-way horizontalmovement each carrying avernier scale reading, to0-1 mm. Movement in allthree directions is smooth,precise,and sensitive, whileworkmanship throughoutis of the same high standardas is shown in the well

known range of PriorMicroscopes.

W. R. PRIOR & CO. LIMITEDLONDON ROAD, BISHOP'S STORTFORD, HERTS.

MICHROME STAINSAND REAGENTS FOR MICROSCOPY, BIOLOGY,

HISTOCHEMISTRY, ETC.D i a i Salts

i Stain

ophe(Pyrrol) Blue

AdenosineAdenylic acidsAzur, A, B, & CBrilliant Cresyl BlueBromophenol RedCarminlc AcidCelloldinCytase Lacmoid Riboni

A Q U A M O U N T : Synthetic, neutral, moderately <OPTOIL: synthetic, non-sticky i

CRISTALITE & CLEARMOUNT: synthetic, CO62-page Price List available

LipaseLuxol Fast BlueMannite, BactMethyl GreenPeptonePi ase

Rosolic AcidSodium a-Naphthyl

PhosphateSudan BlackToluldine BlueTPNTrypsinUrease.Etc.

ntants

EDWARD GURR LTD.42 UPPER RICHMOND ROAD WEST, LONDON, S.W. 14

Cables: MIcromlabs, London Telephone: Prospect 8051 and 7606UNIFORM HIGH QUALITY, SERVICE, RELIABILITY and IMMEDIATE DELIVERY

Ready SEPTEMBER 1959

Encyclopaedia of Microscopic Stains. By Edward Gurr

About 500 pages, Royal 8vo, price about 95s.

Now ready: Methods of Analytical Histology and HistochemistryBy Edward Gurr. 335 pages, Royal 8vo, price 70s.

[5]

The Journal of

Experimental BiologyEdited by V. B. WIGGLESWORTH and J. A. RAMSAY

l | The official medium for the publications of the Society forExperimental Biology, including articles on experimentalbiology and comparative physiology, whether zoological orbotanical.<j The Society is affiliated to the Biological Council and theJournal is included in the Council's 'Contents Lists' service.1$ The subscription rate is £4. 45. net per volume. Singlenumbers cost £1. 10s. net, double numbers £3 net each.

<I The current issue is Volume 36, No. 2, June 1959.

CAMBRIDGE U N I V E R S I T Y PRESSBentley House, 200 Euston Road, London, N.W. 1

The Journal ofGeneral MicrobiologyEditors: B. C. J. G. KNIGHT and A. F. B. STANDFAST

Thejournal of General Microbiology is conducted by the Society for General Micro-biology, whose aim is to promote the advancement of microbiology by providing acommon meeting ground for those working in all the various specialized fields,including, for example, medical, veterinary, agricultural and economic microbiology.The Society is concerned with the more fundamental aspects of the study of bacteria,viruses, micro-fungi, protozoa and microscopic algae in their various biologicalactivities, its members being united by the common aim of developing scientificknowledge concerning micro-organisms.

The subscription rate is 901. net per volume of 3 parts. Single parts 35s. each, pluspostage. Two volumes will be issued each year. Orders should be sent to

CAMBRIDGE UNIVERSITY PRESSBENTLEY HOUSE, 200 EUSTON ROAD, LONDON, N.W.I

[6]

MSE MICROTOMES

The 'BASE SLEDGE'THE MSE 'BASE SLEDGE' MICROTOME is

particularly rigid in construction allowing forthe smooth sectioning of even the toughestspecimens. Object feed is adjustable from1 to 20 microns in steps of 1 micron. Takes

240 mm. knife. A variety of stages forparaffin, celloidin or freezing work isavailable.

The 'LARGESECTION'

MSE'LARGE SECTION' MICRO-TOME (According to Gough andWentworth): This new model makespossible a new technique in sectioning ofspecimens up to 11" X 9" for direct obser-vation. Object feed adjustable to anythickness, with positive indexing at every100 ft. Takes 350 mm. knife.

(Full details inleaflet No. 101)

{Full details in leaflet No. 104)

MEASURING AND SCIENTIFIC EQUIPMENT LTD., Spenser St., London, S.W.I

[BAKER)The TRILUX CONDENSER

forPHASE CONTRAST,

DARK FIELD,and

LIGHT FIELDmicroscopy

without changing objectivesor condenser

Full particulars on request

C. BAKER INSTRUMENTS LTDMETRON WORKS • PURLEY WAY • CROYDON • SURREY CROydon 384J-6-7-8

SPECIFY.

DIFCOmicrobiological

reagents and mediaCULTURE MEDIA

MICROBIOLOGICAL ASSAY MEDIA

TISSUE CULTURE AND VIRUS MEDIA

SEROLOGICAL REAGENTS • ANTISERA

DIAGNOSTIC REAGENTS

SENSITIVITY DISKS • UNIDISKS

PEPTONES • HYDROLYSATES • AMINO ACIDS

ENZYMES • ENRICHMENTS • DYES • INDICATORS

CARBOHYDRATES • BIOCHEMICALS

Difco LaboratoriesOver 60 years' experience assures

UNIFORMITY • STABILITY • ECONOMY

Prompt delivery from U.K. stock. Write for Difco Manual andtechnical leaflets to the sole agents:

BAIRD & TATLOCK (LONDON) LTD., CHADWELL HEATH, ESSEX, ENGLANDBranches in London, Manchester, and Glasgow. Agents throughout U.K. and all over the world

complete laboratory service

[8]