the power of small2014.igem.org/files/presentation/virtus-parva_mexico... · 2014-11-25 · some...
TRANSCRIPT
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The Power of Small
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The Bio-NEMS drill
*Patent pending
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Resonator
*Patent pending
Image obtained from MEMS & Nanotechnology Exchange.
<https://www.mems-exchange.org/MEMS/what-is.html>
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DNA Coiling
*Patent pending
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Biological Resonator?*Patent pending
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IDEA: Mimic the chromatin’s nucleosome
*Patent pending
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Survey
What is a
Microelectromechanical
System (MEMS)?
NEMS
28.77
71.23
Know what a MEMS is
Do NOT know what a MEMs is
*Patent pending Fedder, G.K. ; Carnegie Mellon Univ., Pittsburgh ; Howe, R.T. ; Tsu-Jae King Liu ; Quevy, E.P.
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Movie Projection
Disseminate
Nanotechnology and
its contribution to
Synthetic Biology
*Patent pending
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Some assumptions were made:
• All NPs are monocrystalline and equal
(Fe3O4, r=11nm and Fe3O4@SiO2, r= 21nm)
• The DNA coiled around acts as a rigid body.
• The force to unbind DNA from the protein is
about 6.3 pN.
6.3 pN
*Patent pending
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Some assumptions were made:
• All NPs are monocrystalline and equal
(Fe3O4, r=11nm and Fe3O4@SiO2, r= 21nm)
• The DNA coiled around acts as a rigid body.
• The force to unbind DNA from the protein is
about 6.3 pN.
• DNA mass is insignificant compared to the
NP’s.
• All drags are the same
• Homogeneous medium
• The system can be reduced to a single mass
and drag
6.3 pN
*Patent pending
Bio – NEMS Drill
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Lorentz force equation
Drag force equation
The amount of Bohr
magnetons per unit cell
Number of paired
electrons
Charge
Total magnetic force
Total force
Amount of
Nanoparticles
*Patent pending
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*Patent pending
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The Bio-NEMS drill
• Individual Components:
• Magnetic Nanoparticles
• Peptide
• Cross-linking agent
• DNA strand
*Patent pending
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NanoparticleSynthesis• Co-precipitation
Method
• More tan 50 trials
• Functionalization• Biocompatible
*Patent pending
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Magnetite
• Superparamagnetic
• 22 nm monodisperse
*Patent pending
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PeptideAssembly• Trial 1: SASP
• Trial 2: HU
• DNA – binding
• Electrophoresis
*Patent pending
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The Bio-NEMS drill
*Patent pending
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Law Initiative
Difficulties with the
importation of reactants
and biological agents!
A law initiative came to be
as a collaboration with the
Tec Monterrey team.
*Patent pending
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Is this real?
*Patent pending
Characterization
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UV Spectroscopy
260 nm
Blue: Np + DNA
Green: Nanoparticles
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Use of plasmids for characterization
of the device
*Patent pending
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Bio-NEMS Drill as
a cloning vector
Improve genetic
transformation
*Patent pending
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*Patent pending
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*Patent pending
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And the Brainstorm came…
*Patent pending
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*Patent pending
The Test
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Does it Work?
*Patent pending
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ResultsBBa_K737051 Used as control
and DNA in our system for a traditional transformation and a Electroporation Method
60 to 80 Colonies in our Control against 80 to 100 in the enhanced electroporation
*Patent pending
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The Joy of Sharing
*Patent pending
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Achievements
Research fund
for students’
projects!!!
Deeper
understanding
of Synthetic
Biology!!!
*Patent pending
Low Budget
project with
great results!!!
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*Patent pending
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What’s next?
*Patent pending
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*Patent pending
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*Patent pending
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IT IS THE REAL
DRILL!!*Patent pending
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iGEM Berlin
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*Patent pending
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*Patent pending
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Marvellous doesn’t always come from complexity