the new icbp animal core facility - arsal
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The new ICBP animal core facility
Ioana Madalina Fenyo, PhD
SPF Animal Core Facility Coordinator
April 23, 2015
Conventional area
SPF Area(Experimental)
SPF Area(Breeding)
Washing area
Quarantine
Platforma de Modelare Experimentala
SPF area - 1215 mouse cages and 258 rat cagesBreedingExperimentalBSL-2 lab (transduction)
Conventional area - 144 hamster/rat cagesBreedingExperimental
SPF Area(Experimental)
SPF Area(Breeding)
IVC racks (Allentown) laminar-flow exchange stations (Allentown)
SPF Area(Experimental)
2 surgery rooms:
- aspirating surgery tables
- Stemi 2000-C stereomicroscopes (Zeiss) with
Digital Microscopy Cameras AxioCam ERc 5s
- gas anesthesia induction systems (Minerve)
temperature regulation module (Minerve)
- CO2 chambers (Minerve)
- light sources (Harvard Apparatus)
- tail vein injection unit (Kent Scientific)
- PowerLab physiological monitoring equipment
(ADInstruments)
- small sterilizer for instruments (Matachana)
- ventilators (Harvard Apparatus, Ugo Basile)
- homeothermic blankets (Panlab)
- microfuge (Hettich)
SPF Area(Experimental)
Vevo 2100® High-resolution Imaging System (VisualSonics)
SPF Area(Experimental)
IVIS Spectrum in vivo Imaging System (Caliper, PerkinElmer)
SPF Area(Experimental)
irradiation unit (RS2000 Rad Source X-ray irradiator)
Leica AM 6000 platform for micromanipulation applications (Leica/Eppendorf)
NSG
Inbred
C57Bl/6
FVB
CBA
Geneticaly modified
apoE KOGSH
Outbred
Wistar
linia TRE-hapoE3
linia Tek-Tet3G
UN MODEL TRANSGENIC, DE TIP CONDIŢIONAL, PENTRU INDUCEREA CONTROLATĂ
A EXPRESIEI APOE3 UMANE ÎN MOD SPECIFIC ÎN ENDOTELIUL VASCULAR
Crearea unui model care să ofere posibilitatea investigării funcţiei apoE în
ateroscleroză şi a modului în care o creştere a concentraţiei acestei proteine la nivelul
plăcii, dar şi în circulaţie, poate ameliora procesul patologic.
Principiul transactivării transcripţionale dependente de tetraciclină – sistemul “tet-on”
Tek/Tie2promoter-Tet3G-Tek/Tie2enhancer(Tek-Tet3G)
TRE-hapoE3
linia TRE-hapoE3 linia Tek-Tet3G
AcknowledgementsOur staff
Conventional area
Emanuel Dragan, VMD – coord.
Emilian Dunana
Lacramioara Costea
SPF area
Madalina Fenyo, PhD – coord.
Safta Nae
Genia Ruse
Paunica Cojan
Serban Morosan, DVM, PhD – UPMC
Francois Lachapelle, PhD
Sebastien Dussaud, PhD
Christelle Enond
Yoann Pain
Dr. Anca Gafencu
CARDIOPRO (143 / SMIS CSNR 2667)
THANK YOU!
AC 1400 washer disinfector (Steelco)
baby bottle washer disinfector with filling station (Steelco)
DS-500 Dump Station (Allentown)
Washing area
Washing area
Two-door autoclave (Matachana) Chemical SAS (Matachana)
Quarantine
InnoSte transfer isolator (EuroBioConcept)Animal transfer station (Allentown)
InnoSte isolator (EuroBioConcept)Animal transfer station (Allentown)