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Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 1
THINKSHOP
THE MILK DAYTRENTO, 4TH FEBRUARY 2015
In dairy industry, one of most pressing unmet needs is the
timely detection of toxins contaminations that originate
from animal feed and are secreted into milk.
The available technology for aflatoxin detection is
laboratory-based, in most cases requires sample
preparation and does not provide timely identification of
contaminants, thus fails to deliver cost-effective
management of milk quality.
Can the use of Micro System Technologies and biosensors
provide a solution to overcome these limitations?
What are the major factors that could jeopardize the
integration and automation of current analysis
techniques?
Milk chemistry and composition, strict regulations for milk
quality, dairy industry requirements, the large diffusion of
milk consumers and producers pose substantial
challenges, but, in the meanwhile, offer a number of
opportunities for exploiting the huge potential of
miniaturized systems and sensors.
This workshop, organized in the framework of the
SYMPHONY project, will be the occasion to discuss relevant
open issues in the application of MST in the analysis of
milk contaminations.
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 2
Program
14.10- Introduction and presentation of SYMPHONY project
Andrea Adami, FBK-Technical manager of the SYMPHONY project
14.30- Milk quality testing and regulations
Ulrich Leist, DRRR, Germany
14.50- Milk and health in the Province of Trento
Alberto Mattivi, Prevention Dep. Local Health Trust, Trento, Italy
15.10- Milk quality testing in Trentino
Nicola Cologna, CONCAST, Trento
15.30- Rapid Detection of Aflatoxin M1 in Milk: Analytical Challenges and Validation Aspects under EC Perspective
Veronica Lattanzio ISPA-CNR, Italy
15.50- Research & Business - Transitioning Innovation to Commercial Success
Mark Whatton, QCL, United Kingdom
16.10- Coffee break
16.40- MST for food quality and safety
Andrea Adami, FBK-Technical manager of the SYMPHONY project
17.00 - Discussion
18.00- Conclusion
FP7-ICT-2013-10 Grant agreement no: 610580
SYMPHONYIntegrated SYsteM based on PHOtonic Microresonators and
Microfluidic Components for rapid detectioN of toxins in milk and diarY products
3Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 4
The aflatoxin issue in milk industry
Aflatoxin M1 is a milk contaminant and potent carcinogenclassified in group 1 of the International Agency for theResearch on Cancer (IARC, 1993).
Aflatoxin B1 is produced by the fungus Aspergillus flavus andcan contaminate feedstock. Aflatoxin M1 is a metabolite ofaflatoxin B1 and is secreted into milk.
Thermally stable during pasteurization or UHT treatment
In the European Commission regulation (EC) No. 1881/2006, themaximum level of aflatoxin M1 contamination in milk is set to50 ppt and to 25 ppt for infant formulae, as a vulnerable groupof the population
Aflatoxin is screened periodically by ELISA or lateral flow kits,with HPLC used as certified method for confirmation of positivecases
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 5
Recent cases in Italy
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 6
The project at a glance
• The objective of the SYMPHONY project is the development of a system for thedetection of aflatoxin M1 in milk for the dairy industry, that can take samplesat various stages during milk production when milk is transferred betweenstorage sites.
• The SYMPHONY project will deliver and test in real settings a smartheterogeneous integrated system by the integration of key enablingtechnologies such as micro-nano-bio-systems (MNBS) and photonics,polymer-based technologies for low-cost microfluidics and surfacefunctionalisation.
• The vision is to develop an analytical system with sensing capabilities to detectaflatoxin M1 but also extensible also for multiple parameters detection.
Research Centers and University
• Fondazione Bruno Kessler, Trento (IT)
• ACREO Swedish ICT (S)
• Department Physics of University of Trento (IT)
Companies
• Lionix BV (NL)
• Epigem Ltd. (UK)
• CONCAST - Consorzio Dei Caseifici Sociali Trentini (IT)
• Quadrachem Laboratories Ltd. (UK)
7 partners - 4 Countries 3 (I), 2 (UK), 1 (NL), 1(S)
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 7
The consortium
http://science.unitn.it/~semicon/
www.acreo.se
http://www.lionixbv.nl/
http://epigem.co.uk/
http://www.trentingrana.it
www.fbk.eu
http://www.qclscientific.com/
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 8
Key Technologies
SYMPHONY project addresses scientific and technical advancement in thefollowing technical fields:
• Biofunctional surfaces for sensors and sample treatment, based on aptamersand antibodies, for the optimisation of sensitivity and limit of detection.
• Development of microfluidic modules for fat removal, aflatoxin extraction,pre-concentration and elution.
• Photonic devices, by the development of new devices based on Micro RingResonators (MRR) and asymmetric Mach-Zender interferometers (aMZI).
• Hybrid integration of photonic sensors in a monolithic photonic system,including VCSEL and photodiodes.
• Integrated heterogeneous systems, by the merging of photonic devices andmicrofluidics for automated systems in milk quality control.
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 9
Milk Chain and Safety Testing at dairies
Compliments existing testing procedures
within dairy industry
Access to experienced technicians to operate and maintain system
Could immediately assist dairies with
meeting legislation
Test time needs to be within 5 minutes so tanker acceptance
times are not increased
Robust system required to survive typical milk
intake laboratory
Rapid uptake due to end user familiarisation with analytical systems
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 10
Sample preparation by continuous flow microfluidics
Fat separation
• pre filtration may be needed to remove non-milk debris and milk "clots"
• Centrifugation replaced by continuous flow fat removal
• Numbering up or manifolded multiple devices provides increased throughput
Concentration
• Immunoaffinity concentration of aflatoxin to:
• Increase the limit of detection of the system
• Reduce the interference of milk matrix
• Release by temperature or pH shock
detection
• Microseal interface to sensor is required
• Sensor based on mRR or MZI with integrated VCSEL, detectors
• A valve controlled sub-system could be used for regeneration of sensor surface using valve control of a sensor flow cell linked to reservoirs on-or-off chip
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 11
Integrated photonic system
The photonic system is based on:
• LioniX Si3N4/ SiO2 single stripe technology and FBK SiON technology
• Sensor architecture: MRR/MZI
• The integration strategy: butt end coupling
• Cross section and sensor overview (planar and vertical coupling)
Workshop: The Milk Day, Trento 4th February 2015 - Symphony Project, www.symphony-project.eu 12
Integration of a compact photonic sensor system
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The challenges
What are the major challenges for microsystems for milk analysis?
1. Milk is a complex matrix, including multiple phases and a number of chemical species.
Casein micelles, typical size is 62-154nm, fat globules, also including proteins,
phospholipids, glycoproteins, neutral lipids, enzymes and other minor components.
Typical size is about 0.2-8µm, water-based fraction includes whey proteins, salts, sugars,
vitamins. Aflatoxin and device itself interact with the matrix.
2. Simple and fast systems are more likely to meet the requirements (cost and time for
measurement)
3. Robust systems (including calibrations and measurement repeatability)
4. Matching user practices