the linking number (l) of dna - university at buffalokoudelka/lecture4-ppt.pdfthe linking number is...
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![Page 1: The Linking Number (L) of DNA - University at Buffalokoudelka/Lecture4-ppt.pdfthe linking number is 5400/10=540, where 10 is the basepair per turn for type B DNA. Twist is a measure](https://reader035.vdocuments.us/reader035/viewer/2022070722/5f01de4e7e708231d4016d92/html5/thumbnails/1.jpg)
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The Linking Number (L) of DNA
• The linking number of DNA, a topological property, determines the degree of supercoiling;
• The linking number defines the number of times astrand of DNA winds in the right-handed directionaround the helix axis when the axis is constrainedto lie in a plane;
• If both strands are covalently intact, thelinking number cannot change;
• For instance, in a circular DNA of 5400 basepairs,the linking number is 5400/10=540, where 10is the basepair per turn for type B DNA.
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Twist is a measure of the helical winding of theDNA strands around each other.
Given that DNA prefers to form B-type helix, thepreferred twist = number of basepair/10;10 is the number of necleotide in one twist!
The Twist (Tw) of DNA
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The Writhe (Wr) of DNA
•Writhe is a measure of the coiling of the axis ofthe double helix.
A right-handed coil is assigned anegative number (negative supercoiling) anda left-handed coil is assigned a positive number(positive supercoiling).
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Topology theory tells us that the sum of T and W equals to linking number:
L=T+WFor example, in the circular DNA of 5400 basepairs, the linking number is 5400/10=540If no supercoiling, then W=0, T=L=540;
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Zechiedrich et al., J Biol Chem, (2000) 275,8103-8113,