the immune system, anticancer mechanism , enzyme
TRANSCRIPT
ProfessorYuka Itokawa ， ProfessorYeunhwa GU
Prepared by: Japan Suzuka University of Medical Science
30P1-am238 Register # 30P1-am238 Register #
Research Background
In late years, death by cancer, metabolic syndrome, diabetes, heart disease, cardiovascular disease, liver, and kidney have increased.
There is an overall increase of people dying by a malignant neoplasm Overall imbalances of human conditions have increased and enzymes
ingestions has decreased.
IIMethods1 (immunological enhancement effects) Animal used for experiment: Animal used for experiment: ICR mouse (♂) four week ICR mouse (♂) four week An experimental group several:10 group (each eight groups)An experimental group several:10 group (each eight groups)•Dosage: 0.01ml/g Dosage: 0.01ml/g •Medication method: oral administration (every day):Medication method: oral administration (every day):It is distilled water of the same amount in control groupIt is distilled water of the same amount in control group•Administration level: 500mg/kgAdministration level: 500mg/kg•Irradiation condition: 2Gy radiation bath an administration start Irradiation condition: 2Gy radiation bath an administration start two weeks latertwo weeks later (200KV, 9mA, 0,341Gy / min)(200KV, 9mA, 0,341Gy / min)
Methods 2 Experimental groupExperimental group
① ① Control groupControl group ② ② Herbs treated groupHerbs treated group ③ ③ Enzyme treated groupEnzyme treated group ④ ④ Herbs + enzyme treated groupHerbs + enzyme treated group
⑤ ⑤ 2Gy independent irradiation group2Gy independent irradiation group ⑥ ⑥ Herb+ irradiation groupHerb+ irradiation group ⑦ ⑦ Enzyme + irradiation groupEnzyme + irradiation group ⑧ ⑧ Herb + enzyme + irradiation groupHerb + enzyme + irradiation group
Methods 3The SOD measurement We collect blood and measure blood using a measurement kit
from non-irradiation group mouse (after post radiation 15th, 30th) after having centrifuged it.
The peripheral blood cell measurement We collect blood of a caudal vein of a mouse (on three hours
post radiation administration on the irradiation day before, 12 hours, 24 hours, 3rd, 7th, 15th)
We do on 30th and measure white-cell count, lymphocyte counts in an automatic blood corpuscle measuring instrument.
Antioxidation assay NO ２－ TB
XA ２ O ２－・ XOD 37 warm ℃ SOD
H ２ O ２＋ O ２ ２ O ２－・＋２ H ＋ H ２ O ２＋ O ２
Pigment (λ max=530nm)) it is reduced to elements than xihormazan N-TB, and to ‥make self-possession in a dehydrase case
Methods 4 (Anti-cancer effect)
Animal used for experiment: C3H mouse (♂) four weeksIt is the right foot for a tumor (SCC -7) than starting administration two
weeks laterSubcutaneous to a femoral region let take it.Experimental group several:10 groupsDosage: 0.01ml/g Medication method; oral administration (every day): It is distilled water of the same amount in control groupAdministration level: 500mg/kg Irradiation condition: right foot femoral region an after tumor intake
10thWe performed local irradiation to a chisel. (200KV, 9mA, 0,341Gy/min)We irradiate it every 6Gy 1st of *3 times 2Gy in total.
Methods 5
EExperimental groupxperimental group ① ① Control groupControl group ② ② Herb treated groupHerb treated group ③ ③ Enzyme treated groupEnzyme treated group ④ ④ Herb + enzyme treated groupHerb + enzyme treated group
⑤ ⑤ 2Gy independent irradiation group2Gy independent irradiation group ⑥ ⑥ Herb + irradiation groupHerb + irradiation group ⑦ ⑦ Enzyme + irradiation groupEnzyme + irradiation group ⑧ ⑧ Herb + enzyme + irradiation groupHerb + enzyme + irradiation group
Methods 6
Tumor stereometry We measured it more for 30 days every 2nd cancer
inoculation one week later. Tumor volumeTumor weight We resect a tumor than a cancer inoculation after
30th and weigh a tumor, We found tumor inhibition rate.
Tumor inhibition rate (%)= Tumor weight (g) of control group CWTumor weight (g) of specimen group TW
SOD-like activity (after 15th)
This histograph is the image of SOD （ 15days later ） . Each histograph represents the mean value ±SE.
SOD-like activity (after 15th)
0
10
20
30
40
50
60
Groups
An
ti o
xid
en
t a
cti
vit
y (
%) Control
Aloe
Enzyme
Aloe+Enzyme
SOD-like activity (after 30th)SOD-like activity (after 30th)
This histograph is the image of SOD （ 30days later ） .Each histograph represents the mean value ±SE. Analyzed by Dunett-test
SOD-like activity (after 30th)SOD-like activity (after 30th)
0
10
20
30
40
50
60
70
Groups
An
ti o
xid
en
t a
cti
vit
y (
%)
Control
Aloe
Enzyme
Aloe+Enzyme
* *
leukocytes
0
20
40
60
80
100
120
140
160
180
200
220
0 10 20 30 40 50
Time(days)
Th
e n
um
ber
of
leu
kocy
tes
(10
２/μ
l)
Control
Aloe
Aloe+Enzyme
Enzyme
This change in number of leukocytes the blood took from the tail vein This change in number of leukocytes the blood took from the tail vein ICR mice. Each line gram represents the mean value ±SE. Analyzed ICR mice. Each line gram represents the mean value ±SE. Analyzed by Fisher-test. *p<0.05,**p<0.01 vs control.by Fisher-test. *p<0.05,**p<0.01 vs control. （↑（↑ 2Gy whole body 2Gy whole body irradiationirradiation ））
* *
****
****
****
* * * *
* *
* *
* *
* *
This change in number of ｌ ymphocyte the blood took from the tail vein ICR mice. Each line gram represents the mean value ±SE. Analyzed by Fisher-test. *p<0.05,**p<0.01 vs control （↑ 2Gy whole body irradiation ）
0
20
40
60
80
100
120
0 10 20 30 40 50
Time(days)
Th
e n
um
ber
of
lym
ph
ocy
te (
10
2 /μl)
Control
Aloe
Aloe+Enzyme
Enzyme
* *
* *
* *
* *
* * * *
ｌ ymphocyte
Effect on anti-tumor by the sample medication. Each line represents the mean value ±S.E Analyzed by Dunnett-test.
* * * *
* * * *
* *
* *
* *
* * * *
* * * * * * * *
* * * * * *
* *
* *
* *
* *
* * * *
* * * * * * * *
Tumor volume(non-irradiation group)
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
0 5 10 15 20 25 30 35
Time(day)
Tu
mo
r V
olu
me
(mm
3 )
controlAloeEnzymeAloe+Enzyme
* * * *
* * * *
* *
* * * *
* * * *
* * * * * * * * * * * *
* *
* *
0.0
2.0
4.0
6.0
8.0
10.0
Groups
Tu
mor
Wei
ght
(g)
Control
Aloe
Enzyme
Aloe+Enzyme
Weight of tumor. Each line represents the mean value ±S.E. Analyzed by Dunett-test (n=7)
Tumor weight(non-irradiation group)
Weight of tumor. Each line represents the mean value ±S.E. It was analyzed by Dunett-test (n=7)
0.0
2.0
4.0
6.0
8.0
10.0
Groups
Tum
or W
eigh
t (g)
Radiation
Aloe＋R
Enzyme＋R
Aloe+Enzyme＋R
Tumor weight(irradiation group)
The free radical removal by SOD
O2O2
O2 O2 --
An injury factorAn injury factor CCHH
OO
DAN polymeraseDAN polymerase
SHSHA replication of DNA errorA replication of DNA error
Chromosomal anomaliesChromosomal anomalies
Approval of autoimmune diseaseApproval of autoimmune disease
An auto-immunity An auto-immunity antigenantigen
A - antibody complexA - antibody complex
BacteriaBacteriaA virusA virus
XODXOD
blood cells, a macrophageWhite blood cells, a macrophageWhite
Stimulation
We remove to We remove to SODSOD
DeactivatiDeactivationon
Reinforcement of removal + biophylaxis ability of a cause tumor inhibition mechanism
Free radical elimination / antioxidation actionFree radical elimination / antioxidation actionThe conduction that detoxication / Radhi Cal The conduction that detoxication / Radhi Cal elimination is enzymaticelimination is enzymaticAnti-inflammatory actionAnti-inflammatory action
DNA disorder (mutation)DNA disorder (mutation)
DNA restorationDNA restoration
A mutation cellA mutation cell
Cytostasis / apotosis conductionCytostasis / apotosis conduction
A cancer cellA cancer cell
Structure of tumor immunity by antigen-antibody reactionStructure of tumor immunity by antigen-antibody reaction
Natural killer Natural killer cellcell
Antigen fault acquired Antigen fault acquired immunityimmunity
ActivationActivationNatural killer Natural killer
cellcell
A cancer cellA cancer cell
An antibodyAn antibodyAn antibody-producing cellAn antibody-producing cell
A killer cellA killer cell
T-helper cellsT-helper cells
B cellB cell
Antigen Antigen presentationpresentation
A macrophageA macrophage Antigen presenting Antigen presenting
cellscells
Antigen acquired Antigen acquired immunityimmunity
A carcinoma antigenA carcinoma antigen