the heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and...
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One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information,
please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
Illumina® is a registered trademark of Illumina, Inc.
© Copyright 2020, New England Biolabs, Inc.; all rights reserved.
The NEBNext® Ultra™ II workflow lies at the heart of NEB’s portfolio for next
gen sequencing library preparation. With specially formulated master mixes and
simplified workflows, high quality libraries can be generated with low inputs and
reduced hands-on time.
As sequencing technologies improve and applications expand,
the need for compatibility with ever-decreasing input amounts
and sub-optimal sample quality grows. Scientists must balance
reliability and performance with faster turnaround, higher
throughput and automation compatibility.
NEBNext Ultra II modules and kits for Illumina® are the
perfect combination of reagents, optimized formulations and
simplified workflows, enabling you to create DNA or RNA
libraries of highest quality and yield, even when starting from
extremely low input amounts.
The Ultra II workflow is central to many of our NEBNext
products, including:
• Ultra II DNA & FS DNA Library Prep
• Enzymatic Methyl-seq
• Ultra II RNA & Directional RNA Library Prep
• Single Cell/Low Input RNA Library Prep
• Module products for each step in the workflow
The Ultra II workflow is available in convenient kit formats or
as separate modules – it is easily scalable and automated on a
range of liquid handling instruments.
To learn more about why NEBNext is the choice
for you, visitNEBNext.com.
The heart of the matter.
The NEBNext Ultra II workflow has been cited in
thousands of publications, as well as a growing number
of preprints and protocols related to COVID-19. Citation
information and extensive performance data for each
product is available on neb.com.
NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645)
Module
NEB #E7546
Module
NEB #E7595
Component of
NEB #E7103
Module
NEB #M0544
Component of
NEB #E7103
NEBNext Ultra II Library Prep with Sample Purifcation Beads (NEB #E7103)
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5 FEBRUARY 2021 • VOL 371 ISSUE 6529 641 SCIENCE sciencemag.org/custom-publishing
Produced by the Science/AAAS Custom Publishing Office LIFE SCIENCE TECHNOLOGIES
new products: dna-rna analysis
NGS Cancer Panels
Oxford Gene Technology (OGT) has expanded
its SureSeq range of NGS panels to facilitate the
interrogation of genes involved in breast and
ovarian cancer and myeloid disorders. The two
new panels include a comprehensive 70-gene myeloid panel and
a 7-gene breast and ovarian cancer panel that incorporates copy
number variation (CNV) detection. Facilitated by OGTís expert bait
design and complimentary Interpret NGS analysis software, the
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detect structural variants such as FLT3-ITDs and KMT2A-PTDs. The
SureSeq Breast Cancer + CNV panel targets BRCA1, BRCA2, ATM,
TP53, CHEK2, PALB2, and PTEN, and can detect CNVs ranging from
single exons to full gene deletions and duplications in these genes
as well. For customers interested in a larger breast or ovarian
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to the Breast Cancer + CNV Panel.
Oxford Gene Technology
For info: +44-(0)-1865-856800
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implied. Additional information may be obtained from the manufacturer or supplier.
Electronically submit your new product description or product literature information! Go to www.sciencemag.org/about/new-products-section for more information.
HiFi Sequencing
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and on-instrument data processing for streamlined production of
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reads, Sequel IIe eliminates the need for postprocessing of sequence
data by users and delivers a 70% reduction in overall secondary
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transfer and data-storage needs by as much as 90%. The instrument
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application for generating reference-quality de novo assemblies from
HiFi reads.
PacBio
For info: 877-920-7222
www.pacb.com
High Molecular Weight DNA Extraction
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tunable fragment-length generation, followed by precipitation of the
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from 50 kb to 250 kb for the standard protocol and into the Mb
range for certain sample types when the lowest agitation speeds are
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including nearly complete removal of RNAóand is ready for use in
downstream applications, including long-read sequencing.
New England BioLabs
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www.neb.com
cDNA Library Preparation
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preparation time and improve consistency. Their novel, single-
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Beads. RNA-sequencing (RNA-seq) is becoming the gold standard
for transcriptomics research, which provides valuable insights into
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therapeutic intervention, developmental stage, or tumor state and
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messenger RNA capture.
Roche Sequencing
For info: 800-262-4911
sequencing.roche.com/en.html
Cas9 Proteins for CRISPR Genome Editing
System Biosciencesí products deliver highly controlled CRISPR/Cas9
activity through direct introductionóby transfection, electroporation,
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that have had Cas9 successfully introduced. Or choose our unlabeled
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acts immediately upon entering the nucleus and is rapidly cleared,
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into complete kits with a T7 guide RNA cloning and production vector
for your convenience.
System Biosciences
For info: 888-266-5066
systembio.com
SARS-CoV-2 Real-Time RT-PCR Assay
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vitro diagnostic) marked and intended for qualitative detection of
nucleic acid from the SARS-CoV-2 virus in human oropharyngeal
swab, nasopharyngeal swab, and saliva specimens from individuals
suspected of having COVID-19 or from asymptomatic carriers.
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nucleocapsid (N) gene and ORF1ab; so mutations in spike protein, as
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also for qualitative detection of nucleic acid from the SARS-CoV-2
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where each specimen is collected under observation or by a health
care provider using individual vials containing transport media.
Pooling is desirable because it allows labs to reduce costs and increase
throughput, but it requires a sensitive assay. Automation solutions are
available delivering plug-and-play pooling prior to viral RNA isolation.
PerkinElmer
For info: 800-762-4000
www.perkinelmer.com
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