the heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and...

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One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications. Illumina ® is a registered trademark of Illumina, Inc. © Copyright 2020, New England Biolabs, Inc.; all rights reserved. The NEBNext ® Ultra II workflow lies at the heart of NEB’s portfolio for next gen sequencing library preparation. With specially formulated master mixes and simplified workflows, high quality libraries can be generated with low inputs and reduced hands-on time. As sequencing technologies improve and applications expand, the need for compatibility with ever-decreasing input amounts and sub-optimal sample quality grows. Scientists must balance reliability and performance with faster turnaround, higher throughput and automation compatibility. NEBNext Ultra II modules and kits for Illumina ® are the perfect combination of reagents, optimized formulations and simplified workflows, enabling you to create DNA or RNA libraries of highest quality and yield, even when starting from extremely low input amounts. The Ultra II workflow is central to many of our NEBNext products, including: • Ultra II DNA & FS DNA Library Prep • Enzymatic Methyl-seq • Ultra II RNA & Directional RNA Library Prep • Single Cell/Low Input RNA Library Prep • Module products for each step in the workflow The Ultra II workflow is available in convenient kit formats or as separate modules – it is easily scalable and automated on a range of liquid handling instruments. To learn more about why NEBNext is the choice for you, visit NEBNext.com. The heart of the matter. The NEBNext Ultra II workflow has been cited in thousands of publications, as well as a growing number of preprints and protocols related to COVID-19. Citation information and extensive performance data for each product is available on neb.com. NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) Module NEB #E7546 Module NEB #E7595 Component of NEB #E7103 Module NEB #M0544 Component of NEB #E7103 NEBNext Ultra II Library Prep with Sample Purifcation Beads (NEB #E7103)

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Page 1: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information,

please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

Illumina® is a registered trademark of Illumina, Inc.

© Copyright 2020, New England Biolabs, Inc.; all rights reserved.

The NEBNext® Ultra™ II workflow lies at the heart of NEB’s portfolio for next

gen sequencing library preparation. With specially formulated master mixes and

simplified workflows, high quality libraries can be generated with low inputs and

reduced hands-on time.

As sequencing technologies improve and applications expand,

the need for compatibility with ever-decreasing input amounts

and sub-optimal sample quality grows. Scientists must balance

reliability and performance with faster turnaround, higher

throughput and automation compatibility.

NEBNext Ultra II modules and kits for Illumina® are the

perfect combination of reagents, optimized formulations and

simplified workflows, enabling you to create DNA or RNA

libraries of highest quality and yield, even when starting from

extremely low input amounts.

The Ultra II workflow is central to many of our NEBNext

products, including:

• Ultra II DNA & FS DNA Library Prep

• Enzymatic Methyl-seq

• Ultra II RNA & Directional RNA Library Prep

• Single Cell/Low Input RNA Library Prep

• Module products for each step in the workflow

The Ultra II workflow is available in convenient kit formats or

as separate modules – it is easily scalable and automated on a

range of liquid handling instruments.

To learn more about why NEBNext is the choice

for you, visitNEBNext.com.

The heart of the matter.

The NEBNext Ultra II workflow has been cited in

thousands of publications, as well as a growing number

of preprints and protocols related to COVID-19. Citation

information and extensive performance data for each

product is available on neb.com.

NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645)

Module

NEB #E7546

Module

NEB #E7595

Component of

NEB #E7103

Module

NEB #M0544

Component of

NEB #E7103

NEBNext Ultra II Library Prep with Sample Purifcation Beads (NEB #E7103)

Page 2: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from
Page 3: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from

AAAS.ORG/COMMUNITY

Where Science Gets Social.

Page 4: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from

PUT YOURRESEARCHOUT INFRONT

ScienceSignaling.org

Submit your research:

cts.ScienceMag.org

Twitter: @SciSignal

Facebook: @ScienceSignaling

Page 5: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from

5 FEBRUARY 2021 • VOL 371 ISSUE 6529 641 SCIENCE sciencemag.org/custom-publishing

Produced by the Science/AAAS Custom Publishing Office LIFE SCIENCE TECHNOLOGIES

new products: dna-rna analysis

NGS Cancer Panels

Oxford Gene Technology (OGT) has expanded

its SureSeq range of NGS panels to facilitate the

interrogation of genes involved in breast and

ovarian cancer and myeloid disorders. The two

new panels include a comprehensive 70-gene myeloid panel and

a 7-gene breast and ovarian cancer panel that incorporates copy

number variation (CNV) detection. Facilitated by OGTís expert bait

design and complimentary Interpret NGS analysis software, the

SureSeq Pan-Myeloid panel includes genes such as CEBPA, JAK2,

CALR, and MPL, as well as covering regions containing hard-to-

detect structural variants such as FLT3-ITDs and KMT2A-PTDs. The

SureSeq Breast Cancer + CNV panel targets BRCA1, BRCA2, ATM,

TP53, CHEK2, PALB2, and PTEN, and can detect CNVs ranging from

single exons to full gene deletions and duplications in these genes

as well. For customers interested in a larger breast or ovarian

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to the Breast Cancer + CNV Panel.

Oxford Gene Technology

For info: +44-(0)-1865-856800

www.ogt.com/ngs_products

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this

space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not

implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/about/new-products-section for more information.

HiFi Sequencing

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and on-instrument data processing for streamlined production of

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reads, Sequel IIe eliminates the need for postprocessing of sequence

data by users and delivers a 70% reduction in overall secondary

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transfer and data-storage needs by as much as 90%. The instrument

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application for generating reference-quality de novo assemblies from

HiFi reads.

PacBio

For info: 877-920-7222

www.pacb.com

High Molecular Weight DNA Extraction

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��� �� ���(%��&���(�� �#���$����������������%&���&��(���� (�(�

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tunable fragment-length generation, followed by precipitation of the

(!�&%(�������'�� (�����&%(�����&��(���&����(&�����������(��&'�(��

from 50 kb to 250 kb for the standard protocol and into the Mb

range for certain sample types when the lowest agitation speeds are

��(��������(����������(%��(�(���'� �� ���(����� �(!%(��('�������

including nearly complete removal of RNAóand is ready for use in

downstream applications, including long-read sequencing.

New England BioLabs

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www.neb.com

cDNA Library Preparation

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�'��������(� ���*�(�(��)������'�����(��'���)*���*�*�(��*) �(�'�

�)���'(��)����(')���)'��&�)'(��������(��&'(����)�&)�����'���

preparation time and improve consistency. Their novel, single-

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�'��&�'��������& '��)(��(���'���)���& �'������'��(��")����'�'$(�

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Beads. RNA-sequencing (RNA-seq) is becoming the gold standard

for transcriptomics research, which provides valuable insights into

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therapeutic intervention, developmental stage, or tumor state and

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messenger RNA capture.

Roche Sequencing

For info: 800-262-4911

sequencing.roche.com/en.html

Cas9 Proteins for CRISPR Genome Editing

System Biosciencesí products deliver highly controlled CRISPR/Cas9

activity through direct introductionóby transfection, electroporation,

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���.�-�#!%��),�*�"���$�����.,������,�� ,��-�,-���+�..-�-�����+.����

that have had Cas9 successfully introduced. Or choose our unlabeled

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���-+�.,����+,�� ,��-����-,���#!%�$�����.&+�.-�,��.�-�����.�

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acts immediately upon entering the nucleus and is rapidly cleared,

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!�������!%��),�*�"����,-��!%��),�*�!%��,�.�,�,��,��.��-��.��

into complete kits with a T7 guide RNA cloning and production vector

for your convenience.

System Biosciences

For info: 888-266-5066

systembio.com

SARS-CoV-2 Real-Time RT-PCR Assay

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vitro diagnostic) marked and intended for qualitative detection of

nucleic acid from the SARS-CoV-2 virus in human oropharyngeal

swab, nasopharyngeal swab, and saliva specimens from individuals

suspected of having COVID-19 or from asymptomatic carriers.

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nucleocapsid (N) gene and ORF1ab; so mutations in spike protein, as

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also for qualitative detection of nucleic acid from the SARS-CoV-2

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where each specimen is collected under observation or by a health

care provider using individual vials containing transport media.

Pooling is desirable because it allows labs to reduce costs and increase

throughput, but it requires a sensitive assay. Automation solutions are

available delivering plug-and-play pooling prior to viral RNA isolation.

PerkinElmer

For info: 800-762-4000

www.perkinelmer.com

Page 6: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from

GOLD OPEN ACCESS, DIGITAL , AND FREE TO ALL READERS

As AAAS’s frst multidisciplinary, open access journal, Science Advances publishes

research that refects the selectivity of high impact, innovative research you expect

from the Science family of journals, published in an open access format to serve

a vast and growing global audience. Check out the latest fndings or learn how to

submit your research: ScienceAdvances.org

Pushing the Boundaries of Knowledge

Page 7: The heart of the matter.€¦ · therapeutic intervention, developmental stage, or tumor state and ) ... and saliva specimens from individuals suspected of having COVID-19 or from

Immune Cells Research CRO

Discover more | www.promab.comDiscover more | www.promab.com

All products are for research only

Advancing All Things Immunology

Custom Research Solutions

Isolated Human Primary Cell Subsets

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Antibody Development

Protein Expression

Technology Licensing

ProMab Biotechnologies offers many different products and services to advance immuno-oncology

research. With a wide offering of high quality human primary cells or engineered CAR-T cells,

ProMab can help be your supplier or establish a proof-of-concept for your immune cell research.

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