the first benchtop next-generation sequencer - ion proton™ sequencer
DESCRIPTION
Powered by Ion Torrent™ semiconductor chip technology, the Ion Proton™ Sequencer is the first benchtop next-generation sequencer to offer fast, affordable human genome and human exome sequencing. Powered by the next generation of semiconductor sequencing technology and offering a similar single-day workflow to the Ion PGM™ Sequencer, the Ion Proton™ Sequencer promises to change the way scientists look at genomics. For more information visit: http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Sequencing/Semiconductor-Sequencing/proton.html?CID=Protonbrochure-SS-12312TRANSCRIPT
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Ion Proton™ SystemIon Proton™ SystemyyJanuary 2012
The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Highly Disruptive Technologies E EEmpower Everyone
Main Frame Mini Computer Personal Computer
Technology generations are defined by who can use themSanger Sequencing Next‐Gen Sequencing Ion Semiconductor Sequencing
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Technology generations are defined by who can use them
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Semiconductors Disrupt Industries
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Ion’s Semiconductor Chip Sees Chemistry
Leverages $1 Trillion investment and $50 Billion annual spend
Ion Semiconductor Chip Digital InformationBiological Information
Leverages $1 Trillion investment and $50 Billion annual spend
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Ion PGM™ Sequencer: The Fastest Selling Sequencer in the WorldThe Fastest Selling Sequencer in the World
• Speed:1 5 hour runsSpeed:1.5 hour runs
• Scalability:10 Mb to 1 Gb
• Simplicity: AutomatedSimplicity: Automated workflows, benchtop convenience
Aff d bl• Affordable
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The Promise of Semiconductor Sequencing First 100 Fold Scaling Delivered and More
A hi d i 2011
First 100-Fold Scaling Delivered and More
Achieved in 2011• 100-fold scaling and 200 bp kits,
525 base perfect reads achievedIon 318™ Chip*
• Breakthrough Ion AmpliSeq™ app, microbial and RNA-seq apps
• 5,000 member Ion CommunityIon 316™
Chip
2012 Roadmap• 2 x 200 paired end kit, 400 bp kits
Ion 314™ Chip
• Custom and fixed AmpliSeq™ panels
• FDA submission and CE-IVD certificationcertification
6 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Introducing Ion Proton™ ChipsThe Next 100 Fold ScalingThe Next 100-Fold Scaling
7 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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$500 Exome and $1,000 Genome Sequencing in a Few Hours on the BenchtopSequencing in a Few Hours on the Benchtop
Ion Proton™ I Chip Ion Proton™ II Chip
2 human exomes165 million wells
1 human genome660 million wells
$1,000 per run $1,000 per run
Highest ThroughputHighest Throughput8 The content provided herein may relate to products that have not
been officially released and is subject to change without notice.
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Introducing the Ion Proton™ SequencerThe Benchtop Genome CenterThe Benchtop Genome Center
9 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Introducing the Ion Proton™ SequencerThe Benchtop Genome CenterThe Benchtop Genome Center
10 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Introducing the Ion Proton™ SequencerThe Benchtop Genome CenterThe Benchtop Genome Center
11 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Introducing the Ion Proton™ Sequencer The Benchtop Genome CenterThe Benchtop Genome Center
• Supports Ion Proton™ I and Proton™ II chipsProton II chips
• $149K List Price (USD)– $99K for Ion PGM™ owners
• State-of-the-art electronics to support highest throughput
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…and Rack-Mountable!
Broad Baylor and Yale have already
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Broad, Baylor, and Yale have alreadysigned up for this configuration
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Harnessing a Decade of Moore’s Law in O LOne Leap…
Nature 475 348–352 (21 July 2011)
Ion Proton™ I Chip: 165 million wells (>100-fold more wells than Ion 314™ Chip)
Nature 475, 348 352 (21 July 2011)
( 100 fold more wells than Ion 314 Chip)
14 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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…While Seamlessly Scaling Ion’s PostLight™ S i Ch i tSequencing Chemistry
Ion 314™ Chip Signal Ion Proton™ I Chip SignalIon 314™ Chip Signal Ion Proton™ I Chip SignalNucleotide Incorporation Signal
Same Signal Same Speed
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Same Signal, Same SpeedInternally generated R&D data shown
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Maintaining 200 Base Read Lengths
200 Base Q20 Reads on Ion Proton™ I Chip
TCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATC||||||||||||||||||||||||||||||||||||||||||||||||||TCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATC1 10 20 30 40 50
AAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGACCT||||||||||||||||||||||||||||||||||||||||||||||+|||AAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATG-CCT51 60 70 80 90 100
TTGAAATCGAATCAGGTTGGTATCGCACAGATGCGACGGCACCACATTCT|||||||||||+||||||||||||||||||||||||||||||||||||||TTGAAATCGAA-CAGGTTGGTATCGCACAGATGCGACGGCACCACATTCTTTGAAATCGAA CAGGTTGGTATCGCACAGATGCGACGGCACCACATTCT101 110 120 130 140 150
GCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAG||||||||||||||||||||||||||||||||||||||||||||||||||GCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAG151 160 170 180 190 200
TCA|||TCA201
16Internally generated R&D data shown
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Single Day Workflow with Highest Throughput
Ion Proton™O T h S t
Ion Proton™ Sequencer Proton™ Torrent ServerIon Kits
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OneTouch System
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Streamlined Bioinformatics InfrastructureServer Room & Informaticists in a Box and in the CloudServer Room & Informaticists in a Box and in the Cloud
Ion Reporter™Solution
Proton™ Torrent ServerProton Torrent Server and Torrent Suite Software
18 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Overcoming Data Bottlenecks
Full Genomes in Ion Reporter™ High DataHours vs. Weeks
Single genome sequencing in hours
pSolution
Flexible cloud-based solution to manage
gQuality
More uniform coverage enables higher quality sequencing in hours
greatly eases analysis bottleneck (vs. batch-processing)
solution to manage, annotate, and archive variants of interest for future interpretation
variant calls with less raw data. Longer reads enable better mappability with less raw data
19 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Ion Proton™ System Availability
January 2012 Ion Proton™ System available for quotesJanuary 2012 Ion Proton System available for quotes
Ion Proton™ I Chip to commence shipment (Ion Proton™ II Chip to be available 6 months later)Early Access for 4-unit rack-mounted configuration
Mid-2012Early Access for 4 unit rack mounted configuration
Unrestricted launch of Ion Proton™ Sequencer and standalone Proton Torrent™ Server to commenceEnd of Q3:2012
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Ion Semiconductor SequencingRapid Benchtop Sequencing for AllRapid, Benchtop Sequencing for All
Genes GenomesIon 3 Series Chips Ion Proton™ Chips
1”1.25”
Ion 3-Series Chips Ion Proton™ Chips
Ion PGM™ Sequencer21
Ion Proton™ SequencerThe content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Unprecedented Scaling Every 6 Months
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Enabling All Applications
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5500 Wildfire
24 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Wildfire Offered to Existing 5500 Customers Mid-2012 DeliveryMid-2012 Delivery
Wildfire simplifies workflow and improves economics while retaining ultra high accuracy and pay-per-lane sequencingg g y p y p q g1.Simpler Workflow: 2 hour on flowchip template preparation2.Lower Price / Gb: $25 / Gb guaranteed3.Higher Throughput: > 20 Gb / day throughputg g p y g p
25 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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Purchasing Options for 5500 and SOLiD C tSOLiD Customers
• Ion Proton™ Sequencer + Wildfire upgrade q pg(discounted package for 5500 customers)
• Ion Proton™ Sequencer + 5500 Wildfire(discounted package for SOLiD customers)
• Ion Proton™ Sequencer(discounted, standalone for 5500/SOLiD)
• Wildfire upgrade(list price, standalone for 5500/SOLiD)
26 The content provided herein may relate to products that have notbeen officially released and is subject to change without notice.
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All products mentioned in this presentation are for Research Use Only, t i t d d f i l h th ti di ti
Confidential and Proprietary—DO NOT DUPLICATE27
not intended for any animal or human therapeutic or diagnostic use.