the european and mediterranean plant ... eppo/01_petter_eppo...european and mediterranean plant...
TRANSCRIPT
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THE EUROPEAN AND MEDITERRANEAN PLANT PROTECTION ORGANISATION
IIl settore fitosanitario e l settore fitosanitario e l'EPPO:l'EPPO:contributi e criticacontributi e criticaRoma, Roma, 20112011‐‐0202‐‐2323
Françoise Petter EPPO
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European and Mediterranean Plant Protection European and Mediterranean Plant Protection OrganizationOrganization
• Regional Plant Protection Organization (article IX of the IPPC)
• Creation 1951 by 15 countries
• International cooperation in plant protection (plant quarantine and plant protection products)
In 2011:50 member
countries
In 2011:50 member
countries
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RegionalPlantProtectionOrganizations
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EPPOEPPO
andand
the the
European UnionEuropean Union
27 EU members are all EPPO members
EU prepares regulationsEPPO makes recommendationse.g. regional standards
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• Plant protection productsPromotion of the use of modern, safe and effective
pest control methods.
• Plant quarantinePrevent entry or spread of dangerous pests (plant
quarantine).
EPPO: two main areas of activitiesEPPO: two main areas of activities
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EPPO bodiesEPPO bodies
Working Party on Phytosanitary Regulations
Working Party on Phytosanitary Regulations
Working Party on Plant Protection Products
Working Party on Plant Protection Products
Working Parties are composed of representative of NPPOs
Meet once a year
Give directions to Panels on the work to be carried out and standards to be developed/revised
Review the technical work done by Panels
Executive Committee (chairman, vice chairman + 7 elected country
representative)
Executive Committee (chairman, vice chairman + 7 elected country
representative)
Council (NPPO heads)Council (NPPO heads)
Administrative bodies
Technical bodies
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Working Party on Phytosanitary Regulations
Working Party on Plant Protection Products
EPPO technical bodiesEPPO technical bodies
Active PanelsPhytosanitary MeasuresCPM affairsQuarantine Pests for ForestryPhytosanitary TreatmentsBacterial DiseasesPRA developmentDiagnostics and Quality AssurancePhytosanitary ProceduresJoint EPPO/OIBC Panel on biological control agentsPhytosanitary measures for potatoesAd hoc Panel on NematodesAd hoc Panel on Invasive Alien SpeciesPanel on entomology Panel on virologyAd hoc Panel on Plant Protection Information
Dormant PanelsCertification of Fruit CropsCertification of Pathogen‐tested OrnamentalsCertification of Seed PotatoesAd hoc Panel on the Phytosanitary Risks of
Composted Organic Waste
Active PanelsEfficacy Evaluation of Fungicides and InsecticidesEfficacy Evaluation of Herbicides and Growth
Regulators
General Standards on Efficacy EvaluationAd hoc Panel on Harmonization of Data on Plant
Protection Products
Resistance Panel on Plant Protection Products
Dormant PanelsRodent ControlEnvironmental Risk Assessment of Plant Protection
Products
Good Plant Protection Practice (GPP)
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EPPO PanelsEPPO Panels
Panels are composed of experts nominated by EPPO member
countries
Meet once a year (or twice)
Prepare draft recommendations for the Working Party, most of
which in the form of standards
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EPPO SecretariatThe EPPO Secretariat prepares and runs all
meetings, and coordinates the activity which arises from them. It is responsible for all
publication and information services.
The EPPO Secretariat is based in Paris
EPPO Secretariat staff
Ringolds Arnitis Director GeneralFrançoise Petter Assistant Director Vlasta Zlof Scientific Officer Andrei Orlinski Scientific Officer Anne‐Sophie Roy Information Officer Muriel Suffert Scientific Officer Sarah Brunel Scientific OfficerRobert Sunley Scientific Officer Damien Griessinger Information Technology Officer Madeleine McMullen Managing Editor Eliane Madène
AdministratorMarie‐Christine Ozanon Secretary Jocelyne Karquel Secretary
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EPPO approval procedureEPPO approval procedure
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EPPO: two main areas of activitiesEPPO: two main areas of activities
Plant Protection ProductsPlant Protection Products•Development of Standards (e.g. efficacy evaluation more than
260 Standards, environmental risk assessment, good plant protection practices…)
EPPO database on efficacy evaluation standards: more information
direct access: http://pp1.eppo.org
•Organization of conferences and workshops on themes related to plant protection
““Hot topicsHot topics””
include include minor uses, resistance, comparative minor uses, resistance, comparative
assessment, zonal recognitionassessment, zonal recognition……
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Minor usesMinor uses• Problems in terms of crop protection product
availability for minor crops. • Current EU review of active substances and
the rationalization of uses • Few products available even for some major
usesMinor uses
– crop considered to be of low economic importance at national level (minor crop),
– pest of limited importance on a major crop (minor pest).
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Minor usesMinor usesStandard (PP 1/224)
Describes the principles for determining the requirements for efficacy evaluation for minor uses of plant protection products in a
registration procedure. Provides a definition of minor uses
Provides guidance on where to seek data to demonstrate effectiveness and crop safety, in order to simplify the registration process.
does not cover other minor use considerations such as residues and ecotoxicology
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Minor uses: EPPO standard PP1/257Minor uses: EPPO standard PP1/257Strategy to address efficacy data requirements =
extrapolate from existing registered uses
EPPO standard PP1/257
specific efficacy and crop safety extrapolations
32 extrapolation tables developed so far to accompany this standard
Available on the EPPO Website www.eppo.orgextrapolation table for effectiveness of fungicides pome fruit
Disease Crop: pome and stone fruit
1 Disease species
2Disease group
3 Indicator crops
4Extrapolation to other crops
5 Data from other crops (or
crop groups) that enables
reduced data on the
indicator crops (or no data
*)
6 Data on indicator crops
that permits extrapolation
to other crops (or crop
groups) with reduced data
(or no data *)
Podosphaera leucotricha
PODOLEPowdery mildew Apple Pome fruit Quince, Pear
Venturia inaequalis VENTIVenturia pyrina VENTPI
Scab Apple Pome fruit Loquat EIOJA, Pear
Diplocarpon mespili DIPCMA Leaf spot (or blight) Pear Quince QuincePhoma pomorum PHOMPO Leaf spot Apple Pear, Cherry Pear, Cherry
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Mutual recognition and zonal assessmentMutual recognition and zonal assessment
Core
principles
of
dossier
submission
for
the authorization
of
plant
protection
products
under
the
new EC Regulation 1107/2009 (replacing 91/414/EEC),
Pb
many
of
the
technical
details
for
conducting
zonal authorizations
still
need
to
be
addressed,
agreed
and
harmonized among EPPO member countries.
EPPO Workshop Berlin EPPO Workshop Berlin
5 & 65 & 6
April 2011 April 2011 Deadline for registration
28th
February 2011
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Comparative assessmentComparative assessment• In EU comparative assessment will be required for
authorization of Plant Protection Products, which contain an active substance identified as a candidate
for substitution (Regulation (EC) No1107/2009, Articles 24 and 50).
• Countries
decided
that
EPPO
should
develop
a standard to address this issue.
• Draft standard sent for Country Consultation at the end of 2010 expected to be adopted in 2011.
• Objective provide guidance to determine whether the substitution
of
a
plant
protection
product
is
appropriate in view of agronomic considerations.
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EPPO activities related to honeybeesEPPO activities related to honeybees
• EPPO/Council of Europe risk assessment scheme for honeybees (EPPO Series PP 3 Environmental Risk
Assessment Scheme for Plant Protection Products – Chapter 10: Honeybees)
• Standard on the conduct of trials for the evaluation of side‐effects of plant protection products on
honeybees (PP 1/170). ICPBR ‘Bee Protection Group’
provides the technical
input for both EPPO Standards.
Revised in 2010Revised in 2010
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• Conducted according to EPPO Standard PP1/213 (basis for risk assessment within authorization
process in EPPO Countries)• Objective is to determine whether the proposed
use pattern is acceptable• Two parts:
– resistance risk assessment (evaluation of probability of resistance development and its likely impact)
– resistance risk management (if necessary, strategies to avoid or delay resistance development are
proposed)
EPPO Workshop on Azole fungicides and Septoria leaf blotch control
Harpenden (GB), 2010‐12‐07/09In 2011 Tuta absoluta
Resistance Risk Analysis
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EPPO: two main areas of activitiesEPPO: two main areas of activities
Plant Plant Quarantine Quarantine
Plant quarantine: all activities Plant quarantine: all activities designed to prevent the designed to prevent the
introduction and/or spread introduction and/or spread of quarantine pests or to of quarantine pests or to
ensure their official control ensure their official control [IPPC definition].[IPPC definition].
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Plant Quarantine:Plant Quarantine:
EPPOEPPO’’s missionss missions
Prevent entry and spread of pests (crops, forests, natural environments)
Identify potential risks: Early warning systems to identify emerging risks and maintenance of a database
Evaluate potential risks: Pest Risk AnalysisRecommendations on pests which should be regulated as
quarantine pests (EPPO A1 and A2 Lists) Preparation of standards (e.g. official control standards,
diagnostic protocols, inspection procedures….)
Provide information to EPPO members
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EMERGINGEMERGING PEST PEST EARLY WARNINGEARLY WARNING
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What are emerging plant diseases?What are emerging plant diseases?
• Diseases whose incidence is increasing• Diseases whose geographical range is increasing• New pathogens described by science
Sudden oak death in California
Impact can be quite severe!
NPPO of Spain ‐
International Conference ‘Red palm weevil control
strategy for Europe’
(Valencia, 2010‐05‐05/06)
SPAIN: estimated costs of
official control from 2002 to
2009:
45 500 000 euros
SPAIN: estimated costs of
official control from 2002 to
2009:
45 500 000 euros
What is the
problem?
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Possible causes of emergencePossible causes of emergence
Intensification and diversification of commercial exchanges of plants and plant products
accidental introductions of
pathogens into new regions
accidental introductions of
pathogens into new regions
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Possible causes of emergencePossible causes of emergence
Trade of melon/watermelon
seeds: Acidovorax citrulli
Trade of Castanea wood and trees:Cryphonectria parasitica
Trade of cuttings and young
plants: Puccinia horiana
In practice: the detailed pathways and history of disease introductions are usually difficult to retrace
In practice: the detailed pathways and history of disease introdIn practice: the detailed pathways and history of disease introductions uctions are usually difficult to retraceare usually difficult to retrace
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Possible causes of emergences: Possible causes of emergences: Spread of insect vectorsSpread of insect vectors
Bean golden mosaic virusTomato yellow leaf curl virus
Tomato infectious chlorosis virusBemisia tabaci
The number of
viruses transmitted
by Bemisia tabaci
is increasing
(more than 150
species of
Begomovirus and
several species of
Crinivirus,
Ipomovirus and
Carlavirus). Many
of them have been
recently described
and are associated
with emerging
diseases
The number of
viruses transmitted
by Bemisia tabaci
is increasing
(more than 150
species of
Begomovirus and
several species of
Crinivirus,
Ipomovirus and
Carlavirus). Many
of them have been
recently described
and are associated
with emerging
diseases
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Possible causes of emergencePossible causes of emergence
Progress in science (detection and identification of new pathogens)
Kowalski T (2006) Chalara fraxinea
sp. nov. associated with dieback of ash
(Fraxinus
excelsior) in Poland. Forest Pathology
36(4), 264‐270.
Ash dieback
Caused by Chalara fraxinea
(newly described species)
currently spreading in Europe
But C. fraxinea
is the anamorph
of an already described species,
Hymenoscyphus albidus,
considered as non‐pathogenic,
native, and widespread in
Europe …
Ash dieback
Caused by Chalara fraxinea
(newly described species)
currently spreading in Europe
But C. fraxinea
is the anamorph
of an already described species,
Hymenoscyphus albidus,
considered as non‐pathogenic,
native, and widespread in
Europe …
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Chalara fraxineaChalara fraxinea
First described in 2006 in Poland. Then, symptoms or the pathogen were reported
from other countries:
‐
2007 : Denmark, Germany, Estonia, Latvia, Lituania, Sueden, Switzerland
‐
2008 : Austria, Finland, Hungary, Norway
‐
2009 : Slovenia …
First described in 2006 in Poland. Then, symptoms or the pathogen were reported
from other countries:
‐ 2007 : Denmark, Germany, Estonia, Latvia, Lituania, Sueden, Switzerland
‐ 2008 : Austria, Finland, Hungary, Norway
‐ 2009 : Slovenia …
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www.biosecurity.govt.nz/
Identification of a new
pathogen: ‘Candidatus
Liberibacter solanacearum’
‘Psyllid
yellows’
in
North
America
‘Zebra chip’
in USA, or
‘Papa manchada) in
Mexico and Guatemala
Unknown disease on
tomato (and pepper) in
New‐Zealand
Zebra chip: a new disease of potatoesZebra chip: a new disease of potatoes
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Early warning: the EPPO Alert ListEarly warning: the EPPO Alert List
• Initiated in 1999
• Provides early warning
• Suggests possible candidates for Pest
Risk Analysis
Critically reviewed every year (when alert has been given and noCritically reviewed every year (when alert has been given and no further action taken, pests are deleted after 3 years on the lisfurther action taken, pests are deleted after 3 years on the list)t)
Freely available on the EPPO website: Freely available on the EPPO website: www.eppo.org
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EPPO Alert List: current contentsEPPO Alert List: current contentsFungi Bacteria Viruses
Chalara fraxinea
Fusarium oxysporum f.sp.
lactucae
Melamspora euphorbiae
Phytophthora kernoviae
Phytophthora pinifolia
Phytophthora ramorum
Acidovorax citrulli
Liberibacter solanacearum
Pseudomonas syringae pv.
aesculi
Pseudomonas syringae pv.
actinidiae
Spiroplasma kunkelii
Iris yellow spot virus
Pepino mosaic virus
Tomato apical stunt viroid
Tomato torrado virus
Fusarium oxysporum f.sp. lactucae Iris yellow spot virus
Pepino
mosaic
viruswww.agf.gov.bc.ca
Acidovorax
citrulli
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EPPO Alert List: current contentsEPPO Alert List: current contentsInsects & Nematodes
Insects Insects
Agrilus anxius
Chrysophtharta bimaculata
Diaphania perspectalis
Diocalandra frumenti
Drosophila suzukii
Enaphalodes rufulus
Halyomorpha halys
Keiferia lycopersicella
Leptocybe invasa
Leucinodes orbonalis
Oemona hirta
Psacothea hilaris
Xylosandrus crassiusculus
NematodeNematode
Meloidogyne ethiopica
Diaphania perspectalis
Drosophila
suzukii
Leucinodes
orbonalis
Xylosandrus
crassiusculus
Meloidogyne ethiopica
Keiferia lycopersicella
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EPPO Alert List
www.eppo.or g
It provides
information on: •
distribution,
• host plants,•
biology,
•
damage,•
transmission,
•
pathway,•
possible
risks
www.eppo.or g
It provides information on:
• distribution, • host plants,• biology, • damage,• transmission,• pathway,• possible
risks
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INFORMATION INFORMATION GATHERINGGATHERING
DATA COLLECTION AND STORAGEDATA COLLECTION AND STORAGE
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Collect and store information on pestsCollect and store information on pests
Official pest reports
from NPPOs Literature,
Internet surveys
Stored data
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It contains:
New data on biology, host plants, diagnostic methods and geographical distribution of
quarantine pests and pests of potential quarantine significance
Interception reports
Additions to the EPPO Alert List, etc.
Available by eAvailable by e‐‐mailmail
EPPO Reporting Service: a free monthly newsletter
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EPPO Reporting Service:
example of a first record
Source: NPPO of the Netherlands (2010‐11).
Additional key words: new record Computer codes: ANOLGL, NL
2010/200 First record of Anoplophora glabripennis in the NetherlandsThe NPPO of the Netherlands recently informed the EPPO Secretariat of the first record of Anoplophora glabripennis (Coleoptera: Cerambycidae – EPPO A1 List) on its territory. On 2010-11-16, the identity of the pest was confirmed on the basis of morphological analysis of wing covers and DNA analysis of larval remains which had been found in 1 tree in a lane of Acer pseudoplatanus in the municipality of Almere. In total 16 exit holes and 3 larvae were detected in one tree. Observations indicated that some exit holes had been formed recently, whereas others were estimated to be at least three years old. The exact age of exit holes is still to be determined. Signs of the presence of A. glabripennis have also been detected on 6 adjacent trees in a lane of A. pseudoplatanus trees. These trees are located in an industrial area of Almere and were planted approximately ten years ago. In this area there are no tree nurseries and only a limited number of public or private gardens, but a nature conservation area is situated within the surveillance area of 1 kilometre radius around the infested site. It is considered that the most likely source of the outbreak is wood packaging material from Asia because there are several companies importing goods from Asia in the vicinity. Investigations are being carried out to identify the possible source of this infestation. The following phytosanitary measures aiming at a prompt eradication of A. glabripennis are being taken and include the following:1) Destruction of all symptomatic trees, as well as all deciduous trees and shrubs (with a diameter of 2.5 cm or more) located within a range of 100 m from the infested trees. Each individual plant will be dissected and analysed by the national reference laboratory for any signs of the pest. It is envisaged to finalize tree destruction by January 2011.2) Restrictions on the movement of host plant material (with a diameter of more than 2.5 cm) within a range of 500 m around the infested trees. This material should be officially reported and destroyed according to official procedures. 3) Specific surveillance (also involving specialized tree climbers) will be carried out on an annual basis within a radius of 1 000 m around the affected trees for at least the next four years.The pest status of Anoplophora glabripennis in the Netherlands is officially declared as: Transient – Isolated outbreak, actionable, under eradication.
EPPO Reporting Service – Pests & Diseases
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New EPPO database on pestsNew EPPO database on pestsDatabase which contains information about many plant pests
and invasive alien plants (EPPO A1/A2 pests, EU regulated pests and many other regulated pests for other parts of the world in
total more than 1500 distribution records, more than 2100 for categorization)
‐
Host plants
‐
Geographical distribution
‐
Plant commodities liable to carry quarantine pests
‐
Diagnostic protocols, inspection, eradication, PP1
At present, the earlier version of the database (formerly called PQR) can be downloaded from the EPPO website, but it is now under re‐construction (it
will be an online database)
At present, the earlier version of the database (formerly calleAt present, the earlier version of the database (formerly called PQR) can be d PQR) can be downloaded from the EPPO website, but it is now under redownloaded from the EPPO website, but it is now under re‐‐construction (it construction (it
will be an online database)will be an online database)
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New EPPO database on pestsNew EPPO database on pests
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Geographical distribution, host plants, short reports of EPPO Reporting Service, pictures etc. are stored in the Database (under construction to be
released soon)
Geographical distribution, host plants, short reports of EPPO ReGeographical distribution, host plants, short reports of EPPO Reporting porting Service, pictures etc. are stored in the Database (under constrService, pictures etc. are stored in the Database (under construction to be uction to be
released soon)released soon)
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PEST RISK ANALYSISPEST RISK ANALYSIS
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Potential invasive pests Potential invasive pests PRA & phytosanitary PRA & phytosanitary regulationsregulations
• When new pests are emerging, studies can be done to evaluate whether phytosanitary regulations are
appropriate to prevent introduction and spread
• Pest Risk Analysis can be performed
Risk perception Risk assessment
Risk management
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The objective of PRA is to answer these simple’
questions:
•
Can the pest studied be considered as a quarantine pest?
•
Can phytosanitary measures (prohibitions, restrictions on trade …)
be taken to reduce the risk to an acceptable level?
What is Pest Risk Analysis (PRA)?
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EPPO Standard PM5/3(3) Decision‐support scheme for quarantine pests
Pest Risk AssessmentPest Risk AssessmentProbability of entry (pathways, detection, survival in transit)
Probability of establishment and spread (host plants, vectors, climate)Economic impact assessment (direct/indirect damage)
Is it a quarantine pest?
Pest Risk ManagementPest Risk ManagementIs the risk acceptable?
For each possible pathway evaluate the efficacy of possible phytosanitary measures
Propose management options
InitiationInitiationWhich pest to analyse?
Definition of the PRA areaDoes an earlier PRA exist?
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CLIMEX - Compare Locations (1 species)Saperda candida final
Run on Dec 07 2009 14:45World
No Climate Change / Irrigation: Not Set
0 to <1020 to <3040 to <5060 to <7080 to <9090 to <100
Saperda candida (round‐headed apple tree borer)
North American pest of Rosaceae (including apple)
Climatic zones which are similar to the area of origin and where the pest could establish (CLIMEX studies)
Saperda candida (round‐headed apple tree borer)
North American pest of Rosaceae (including apple)
Climatic zones which are similar to the area of origin and where the pest could establish (CLIMEX studies)
PRA tools Climate matching studies to predict pest establishment
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Performing and reviewing PRA to recommend regulation of pests
EPPO recommendations may then serve as a basis for establishing the EU regulations on plant health.
Council Directive 2000/29/EC on protective measures against the introduction into the Community of organisms harmful to plants or plant products and against their spread within the Community
EPPO recommendations may then serve as a basis for EPPO recommendations may then serve as a basis for establishing the EU regulations on plant health.establishing the EU regulations on plant health.
Council Directive 2000/29/EC on protective measures against the introduction into the Community of organisms harmful to plants or plant products and against their spread within the Community
Any request for addition to the EPPO Lists should be supported by a PRA
PRAs prepared by EPPO member countries
PRAs performed by an EPPO Expert Working Group for PRA
PRAs are reviewed by EPPO Panels and pests are eventually added to the EPPO A1/A2 Lists with recommendations on
management options (phytosanitary measures)
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Other activities in the PRA frameworkOther activities in the PRA framework
•• Prepare a compact PRA scheme for Prepare a compact PRA scheme for quicker decision makingquicker decision making
• Prioritization process for invasive alien plants(new standard der development)
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Development of EPPO StandardsDevelopment of EPPO Standards
To help its member countries EPPO has developed Standards on:
Diagnostic protocols
Eradication /containment programmes
Inspection
Certification schemes
Biological Control Agents
In practice, for potentially invasive species which have newly been introduced, the difficulty is that field or lab experience is lacking
(and takes time to build up) – so how to react promptly?
X
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STANDARDSSTANDARDS DIAGNOSTIC PROTOCOLSDIAGNOSTIC PROTOCOLS
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Laboratory analysis are often performed Laboratory analysis are often performed to detect and identify pests to detect and identify pests
NPPOs perform inspections to NPPOs perform inspections to detect pests: detect pests:
Why a programme on Diagnostic protocols? Why a programme on Diagnostic protocols?
on imported consignments (imported or exported)
for the surveillance of their territory (in fields, nurseries glasshouses….)
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Need
for
a
harmonized
approach
to
diagnostic Need
for
a
harmonized
approach
to
diagnostic methods for regulated pests was recognizedmethods for regulated pests was recognized
In 1998, EPPO started a programme to prepare In 1998, EPPO started a programme to prepare diagnostic protocols.diagnostic protocols.
The
work
is
conducted
by
Panels The
work
is
conducted
by
Panels
composed
of composed
of laboratory
specialists
nominated
by
their laboratory
specialists
nominated
by
their
NPPOs. NPPOs.
EPPO Programme on Diagnostic ProtocolsEPPO Programme on Diagnostic Protocols
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Objectives of EPPO DPObjectives of EPPO DP
•
to ensure correct detection and identification of regulated pests
•
to ensure harmonized methodology in different diagnostic services/laboratories
•
to improve efficacy of inspection in order to ensure that effective measures can be taken
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Preparation of EPPO diagnostic protocolsPreparation of EPPO diagnostic protocols
• First
drafts
prepared
by
an
author
according
to
a common format.
• Drafts
are
reviewed
by
relevant
Panels
and
other EPPO bodies.
• 95
pest
specific
protocols
have
been
approved
and 6 general standards
• Approximately 20 protocols are in preparation.
• EPPO DPs are available on the EPPO Website
www.eppo.org
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EPPO Diagnostic Protocol include EPPO Diagnostic Protocol include Pictures to illustrate symptoms
PSTV
or morphological characteristics
Conidia of Colletotrichum acutatum.
Colony of Ciborinia camelliae on potato dextrose agar (PDA).
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EPPO Diagnostic Protocols include EPPO Diagnostic Protocols include
Pupal cases of Aleurocanthus woglumi (left) and A. spiniferus (right) (with the permission of J.H. Martin)
submarginal row of glandular structures/rangée submarginale
de structures glandulaires
submarginal spines épines
submarginales
vasiform orifice orifice vasiforme
marginal teeth dents marginales
legs pattes
doubled pair of submarginal spines paire double d'épines submarginales
Ditylenchus destructor.
Drawings
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EPPO DP include flow
EPPO DP include flow
diagrams
diagrams
e.g. Guignardia citricarpa
e.g. Guignardia citricarpa
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EPPO EPPO
& &
Quality assurance and Quality assurance and accreditationaccreditation
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NEED FOR HARMONIZATIONNEED FOR HARMONIZATIONDifferences concerning the interpretation of
the Standard ISO/IEC 17025 for plant pest diagnostic laboratories noted between national accreditation bodies in different
countries.
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Achievements so farAchievements so far
2009Standard on the interpretation of ISO 17025 for plant pest diagnosis (including guidance on validation of
tests) PM 7/98 Standard will be revisited in 2012 based on experience
2007Standard on basic requirements for quality
assurance PM 7/84
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““Specific requirements for laboratories preparing for Specific requirements for laboratories preparing for accreditation for plant pest diagnosis activitiesaccreditation for plant pest diagnosis activities””
Core element of the StandardCore element of the Standard
A laboratory preparing for accreditation preparing for accreditation should only use validated testsvalidated tests
Validated test = test with the following performance criteria
analytical sensitivityanalytical sensitivity
analytical specificity analytical specificity
reproducibility reproducibility
repeatability repeatability
Depending on the scope of the test selectivityselectivity may also need to be determined.
Validated tests providing performance criteria are considered asValidated tests providing performance criteria are considered as ““standard testsstandard tests”” (equivalent to "standard methods" in ISO 17025).(equivalent to "standard methods" in ISO 17025).
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““Specific requirements for laboratories preparing for Specific requirements for laboratories preparing for accreditation for plant pest diagnosis activitiesaccreditation for plant pest diagnosis activities””
LaboratoryLaboratory performingperforming a testa test
Test with validation data
Laboratory performs a verification Laboratory performs a verification (to confirm its competence in(to confirm its competence in
performing the test)performing the test)
Test with no orincomplete validation data
Laboratory should produce Laboratory should produce missing validation data missing validation data
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Validation tables mycologyValidation tables mycologyMolecular methods, e.g. hybridization, PCR (including DNA extraction)Remark. This step also includes methods for isolation of DNA from the sample material.
Analytical sensitivity Because the concentration of fungi is never known, determine the maximum dilution of DNA detected. Perform at least 3 experiments with 8 serial dilutions. If consistent results are not obtained after 3 series then additional series should be prepared and tested.
Analytical sensitivity refers to a specific set of test parameters which should be stringently defined and standardised, e.g. brand of PCR reagents (in particular DNA polymerase) and PCR cycle conditions.
Analytical specificity Screen once against a range of related target organisms and relevant non-related target organisms (e.g. phylogenetically close fungi) that might be present in the sample and sample extract. The test results can be supported by ‘in silico’ verification (genomic database comparison).
Selectivity Determine the relative insensitivity of the test to variations of the sample material, e.g. by using different cultivars of the host plant.
Repeatability Perform 3 experiments at above, below and at the limit of detection with a minimum of 8 replicates. This can give indications on the level of certainty of measurement
Reproducibility As for repeatability but with different operator(s) if possible , on different days and with different equipments when relevant
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What about tests included in EPPO diagnostic protocols?What about tests included in EPPO diagnostic protocols?
Not all tests include validation data
Survey conducted in 2008 on the use of tests included in 58 EPPO Diagnostic protocols showed that many tests are
widely used.
these tests are considered as giving appropriate confidence regarding repeatability, reproducibility and analytical
selectivity. +
validation data regarding analytical sensitivity to be produced only
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European cooperation for Accreditation European cooperation for Accreditation (EA) & EPPO (EA) & EPPO
Collaboration initiated in 2007 and Collaboration initiated in 2007 and continued since. continued since.
Joint communication EA EPPO Joint communication EA EPPO 20092009‐‐0202‐‐2323
EPPO has been granted EPPO has been granted ““recognized stakeholderrecognized stakeholder””
status by EA status by EA
20112011‐‐0101
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Future plans in QA areaFuture plans in QA area
• Create a database where validation data can be made available.
• Guidance on how to perform ring testing and proficiency testing
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Other initiatives in the framework of Other initiatives in the framework of diagnosticsdiagnostics
Database on diagnostic expertiseDatabase on diagnostic expertise(regulated pests)
Initiated in 2006 open access since 2007-11
• Encourage collaboration and communication between laboratories
• 70 laboratories from 25 countries in the database (~500 experts)
• Annual confirmation of declared expertise requested.
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OTHEROTHER STANDARDSSTANDARDS
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EPPO Standards: eradication and containmentEPPO Standards: eradication and containment
‘Generic elements for contingency planning’: a rapid and effective response of NPPOs to pest outbreaks (containment/eradication):
o a general frameworko pest specific contingency plans remain to be drafted…
Decision support scheme for eradication (in framework of PRATIQUE)
National regulatory control systems: recommendations on delimiting surveys, sampling, trapping, measures in infested
areas:o
Bursaphelenchus xylophilus
o
Diabrotica virgifera
o
Heterodera glycines
………….
o
Draft Agrilus planipennis (Emerald Ash Borer)
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Inspection procedures Inspection procedures
• Procedures for consignment inspection and place of production
inspection
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Biological control agentsBiological control agents
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Phytosanitary Treatments (PM10):Phytosanitary Treatments (PM10):standards describing the methods to be
followed for treatments of commodities and treatments of crops for containment or
eradication of regulated pests: e.g. Number Title of Standard
PM 10/1(1) Disinfection procedures in potato production
PM 10/2(1) Hot water treatment of Dracaena and
Yucca
cuttings against Opogona sacchari
PM 10/4(1) Sulfuryl fluoride fumigation of dried fruits and nuts to control
various stored product
insects
PM 10/6(1) Heat treatment of wood to control insects and wood‐borne nematodes
PM 10/7(1) Methyl bromide fumigation of wood to control insects
PM 10/8(1) Disinfestation of wood with ionizing radiation
PM 10/9(1) Low energy electron treatment of cereal seed against fungi
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Before I thank you for your Before I thank you for your attention attention
I would like to thank the experts.I would like to thank the experts.
EPPOEPPO’’s achievements are based s achievements are based on collaboration between on collaboration between
experts from our region.experts from our region.
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Thank you!