the effect of electrical stimulation on adipose stem cells cultured in conductive stereolithographic...
TRANSCRIPT
The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in
Conductive Stereolithographic Scaffold Structures
Suvi Haimi, PhDAdult Stem Cell Group, BioMediTech,
University of Tampere, Finland &
Department of Biomaterials Science and Technology, University of Twente, The Netherlands
BioMediTech
• Tissue engineering and stem cell
technology
• Biomaterials
• Sensor and actuator technologies
• Measurement and imaging technologies
• Biotechnology
• Immunology
• Cancer and mitochondrial research
• Systems biology
• Bioinformatics
• Computational methods in biomedicine
BioMediTech is a joint institute of
University of Tampere & Tampere University of Technology
Over 250 scientists in world-class basic and translational research
Tampere, Finland
Adult Stem Cell Group: world leader in stem cell based reconstructions of cranial bones
Example of Research Excellence
Combining stem cells with
biomaterial
Isolation of stem cells
GMP culture for 2-6 weeksTransplantation
back into same patient
Combination of biomaterials and stem cells differentiated from subcutaneous fat
Harvesting of adipose
tissue
Department of Biomaterials Science and Technology
University of Twente
Enschede, The Netherlands
Stereolithography
• Polymer synthesis
• Polymer engineering
• State-of-the-art scaffold processing techniques
Mechanical stress
CompressionStretch
Fluidflow
+-
Electrical stimulation (ES) in skeletal tissue engineering
Wound healing
Embryonic development
Action potential
• Used in numerous biomedical applications
• Bioactive – can be easily incorporated with negatively charged biomolecules
Electrically conductive polypyrrole (PPy)
J Biomed Mater Res A, 2009
Langmuir, 2013
Tissue Eng Part A, 2013
Ann Biomed Eng, 2014
Biocompatibility of PPy in vitro
Our team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Non-Charged PPy (0)
100 µm
100 µm
Biocompatibility of PPy in vitro
My team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Uncharged PPy (0)
100 µm
100 µm
• Charging increased cell surface area of ASCs at 3h
PPy promotes smooth muscle differentiation
Björninen et al. submitted
Calponin/DAPI MHC/DAPI SMA/DAPI
Aim
Differentiate hASCs towards skeletal tissues applying ES via designed poly (trimethylene
carbonate) (PTMC) scaffolds coated with PPy
Study design
Electroactive polypyrrole coating
3D electrical stimulation
Designed 3D scaffolds
Adipose stem cells
Control ES 7d - rest 7d
ES 4h/d ES 8h/d0
1
2
3
4
5
6
7
α-SMA
Calponin
MHC
Re
lati
ve
ex
pre
ss
ion
at
14
d
/Electrical stimulation in 2D- Effects on smooth muscle differentiation
*
*
control ES 4h/d ES 8h/d0.00
0.50
1.00
1.50
2.00
2.50
α-SMASM22αMHC
Re
lati
ve
ex
pre
ss
ion
at
14
d
Electrical stimulation in 3D-Effects on smooth muscle differentiation
Electrical stimulation in 3D enhanced ASC osteogenic differentiation
*
*
14
d
ControlES
14 d
Summary
• PPy-coated scaffolds are potential for skeletal tissue engineering
• ES is potential method to engineer muscle and bone tissue
• Screening of more effective ES parameters in 3D environment needed
11.12.2008
Acknowledgements
TAMPERE UNIVERSITY OF TECHNOLOGY
Funding• Academy of Finland
• TEKES, Finnish Funding Agency for Technology and Innovation
• Pirkanmaa Hospital District Competitive research funding, EVO
• The City of Tampere
Adult Stem Cell GroupDepartment of Biomaterials Science and Technology
Key collaborators•
• Tampere University of Technology, Finland• VTT Technical Research Center,
Finland
Kiitos / Thank You!