the daly lab
DESCRIPTION
The Daly Lab. Personnel: Postdoctoral: Erich Staudacher Graduate students: Oakland Peters Esther Mwilaria (MS Sp 07) Undergraduate students: Faizan Kalwar Mandy Hatfield Morgan Bridi Tyler Pittman Aarzu Moghal Sawan Phrabhu. Current proposals: - PowerPoint PPT PresentationTRANSCRIPT
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The Daly Lab
Personnel:Postdoctoral:
•Erich Staudacher Graduate students:
•Oakland Peters•Esther Mwilaria (MS Sp 07)
Undergraduate students: •Faizan Kalwar •Mandy Hatfield •Morgan Bridi •Tyler Pittman•Aarzu Moghal •Sawan Phrabhu
Current proposals:• National Institutes of Health (R01) – NIDCD “The role of
periodic input in odor coding” PI: Kevin C. Daly 5 years; (initial score 228; in review).
• Human Frontiers in Science. “The virtual 4D antennal lobe: Representation of odor processing in space at spike-time resolution. Score: 30th (in revision for NSF ETA spring 09).
Prior funding:• National Institutes of Health (R03) – NIDCD: “Analysis of
combinatorial olfactory coding” Grant number: DC05535; 3 years, $221,000.
• McDonnell Foundation collaborative grant: “Hydrodynamic and neural filtering of spatial information in a turbulent odor plume” 3 years $105,000.
• National Institutes of Health (R01) – NIDCD “Aminergic modulation underlying olfactory plasticity” PI: Thomas A Christensen 5 years; OSU subcontract: $200,000 Grant number: DC05652.
• National Institutes of Health (P20) – NCRR “COBRE in Sensory Neuroscience” PI George Spirou; CoPI: Kevin C. Daly (RR015574-06). 5 year $10,724,104.00: Daly sub award: $1,354,457.00
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Wing beating causes oscillating airflow over the antennae
Sane and Jacobson JEB 2006
Indu
ced
airs
peed
(m
/s)
Rel
ativ
e am
plitu
de
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EA
G D
efle
ctio
n (m
V)
Peri-stimulus Time (sec)
2
1
0
-1
-2
2
0
-2
4
-4
i2
1
0
-1
-2
2
1
0
-1
-2
2
0
-2
4
-4
2
0
-2
4
-4
i
-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5
2
1
0
-1
-2
4
0
-4
8
-8
ii2
1
0
-1
-2
2
1
0
-1
-2
4
0
-4
8
-8
4
0
-4
8
-8
ii
20 Hz
Continuous
Frequency (Hz)
Raw
Po
we
r (m
V2 /
Hz*
10-
3 )
10 20 30 40 5010 20 30 40 50
10
5
0
10
5
0
i
16 18 20 2216 18 20 22
ii
10 15 18 20 22 25 30 34 4010 15 18 20 22 25 30 34 40
4.5
3.5
2.5
1.5
.5
-.5
4.5
3.5
2.5
1.5
.5
-.5
Stimulation frequency (Hz)A
vera
ge
po
wer
(z-
scor
e) *̂*̂
*̂*̂*̂*̂ *̂*̂ *̂*̂ *̂*̂
^̂
Oscillating odor stimulus results in oscillating electroantennogram (EAG) up to ~30Hz
RAW and 5Hz high pass filtered EAG
Power spectral densityIntegrated power
Mean integrated powerpulsed odorpulsed blank3s cont. odor
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Oscillating stimulus results in oscillating local field potentials in the antennal lobe up to 72 Hz
Frequency (Hz)
Raw
Po
wer
(m
V2 /
Hz*
10-3)
10 20 30 40 5010 20 30 40 50
2
1
0
2
1
016 18 20 22
i ii
10 14 18 20 22 25 30 34 4010 14 18 20 22 25 30 34 40
1.5
.5
-.5
1.5
.5
-.5
Stimulation frequency (Hz)M
ean
pow
er (
z-sc
ore)
**
*̂*̂
^̂
^̂ *̂*̂^̂ *̂*̂ *̂*̂ *̂*̂
Ext
race
llula
r Pot
entia
l (m
V)
Peri-stimulus Time (sec)
2
1
0
-1
-2
2
1
0
-1
-2
i
2
1
0
-1
-2
2
1
0
-1
-2-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5
ii
2
0
3
1
-1
-2
-3
2
0
3
1
-1
-2
-3
2
0
3
1
-1
-2
-3
2
0
3
1
-1
-2
-3
i 20 Hz
Continuous
Mean integrated powerpulsed odorpulsed blank3s cont. odor
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Peri-stimulusTime (sec)
Pro
babi
lity
0 0.2 0.4 0.6 0.8 1 1.2Time (sec)
00.10.20.3
Pro
babili
ty
0 .2 .4 .6 .8 1 1.2
0.1.2.3
Odo
r
A6A7A8A9
A10K6K7K8K9
K10B 10 20 30 40 50 10 20 30 40 50
Mea
n P
ower
(z-
scor
e) 1
.5
0
-.5
-1
Frequency (Hz)
i ii
Uni
ts R
espo
ndin
g (%
)10 14 18 20 22 25 30 34 40
Stimulation frequency (Hz)
50
40
30
20
10
0
stim
Oscillating stimulus results in oscillating unitary spiking from antennal lobe neurons up to ~40 Hz (extracellular)
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Oscillating stimulus results in oscillating unitary spiking from antennal lobe neurons up to only ~15 Hz (intracellular)
1
0
-1
1
0
-1
10 20 30 40 5010 20 30 40 50Mea
n P
ow
er (
z-sc
ore)
Frequency (Hz)
10 20 30 40 5010 20 30 40 50Mea
n P
ow
er (
z-sc
ore)
1
0
-1
1
0
-1
Frequency (Hz)
Local interneuron
Projection neuron
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GABAA disruption results in loss of oscillating responses from antennal lobe LFP and unitary spiking
10 20 30 40 465
50
40
30
20
10
0
50
40
30
20
10
0Uni
ts R
espo
ndin
g (%
)
Stimulation Frequency (Hz)
Loss of unitary oscillatory responses
10 20 30 40 46 57 7210 20 30 40 46 57 72
.8
.6
.4
.2
0
-.2
-.4
-.6
.8
.6
.4
.2
0
-.2
-.4
-.6Mea
n po
wer
(z-
scor
e)
Stimulation Frequency (Hz)
* * * * * * *
-.5 0 .5 1 1.5
100
80
60
40
20
0
100
80
60
40
20
0
Peri-stimulus Time (sec)M
ean
pow
er (
z-sc
ore)
Fre
quen
cy (
Hz)
i
ii
10
0
8
6
4
2
10
0
8
6
4
2
pre bicucullinepre
bicuculline
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Reconstructing whole antennal lobe output responses by serial reconstruction
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Virtual population responses from confirmed PN in response to an odor panel
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