the autoimmune paradigm
DESCRIPTION
Probiotic Yogurt and Experimental Arthritis Ehud Baharav Abraham Weinberger Department of Medicine B, Rabin Medical Center, Campus Beilinson. The Laboratory of Physiopathology of Joints and Inflammation, Felsenstein Medical Research Center. The Autoimmune Paradigm. Genetic. Genetic. - PowerPoint PPT PresentationTRANSCRIPT
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Probiotic Yogurt and Experimental Arthritis
Ehud Baharav Abraham Weinberger
Department of Medicine B, Rabin Medical Center, Campus Beilinson.
The Laboratory of Physiopathology of Joints and Inflammation, Felsenstein Medical Research Center
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The Autoimmune Paradigm
โขPhysical Sun
โขFood
โขPregnancy
โขEmotional distress
Genetic
Genetic
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Rheumatoid Arthritis
Early RA
Termography
Advanced RA
X-Ray Advanced RA
End Stage RA
Early RA
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Adjuvant Arthritis on Day 24
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Chronic Arthritis at 6 Month
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ืืกืืจืชืืช ืจืคืืื ืกืื ืืช
ืืื ืืจืคืืื ืกืื ืืช ืืืืื ืืขืืืจ ืืืืงืช ืคืจืงืื?
ืืืื ืื ืชืืืื ืืจืคืืื ืืืฉืืืื ืืจืคืืื ืืกืื ืืช ืืื ืืขืชืืงื,ืื ืคืืฆื ืืื ืจืื ืื ืืืขืืื ืืืืชืจ ืืฉืจ ืืืคืืช ืืืจ ืืืคื ืฉื ืื ืืืืืืื ื ืื ืฉืื ืืจืืื ืืขืืื.
ืืืืฆืืช ืืืงืื ืขื ืืกืืืคืืืืื ืฉื ืืืงืืช ืืคืจืงืื
- ืืืืื ืข ืืฉืืื ืืืืฉืืช ืืกืืืื ืืื. - ืชืจืืืื ืืชืขืืืืช,ืขืืืฃ ืืืื ืื ืื ืืฉ ืคืืืช ืขืืืก ืขื ืืฉืจืืจืื.
- ืืืืืืืช ืืืืช. - ืืจืืื ืืืฉืงื
ืืืืฆืจื ืืื ืืืืืจืื ืืช ืืืฆืืชืืื ื ืืชืืืื,ืืืื ืืืืื ืฉืืื ื -
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ืจืคืืื ืืืขืืช
ืืืืฆืืช ืืชืืื ื ืืจืืื ืื ืืฉื ืืืงืช ืืคืจืงืื ืืชืื "ืืื ืฆืืงืืืคืืื ืืืืฉื ืืจืืคืื ืืืขื"
)N.D( ืื"ืจ ืืืจืง ืกืื ืืืจ )M.Dืืืช ื"ืจ ื'ืืืืก ืืืื )
:ืืืื ืืช ืฉืืฉ ืืืืื ืข ืืืืืืืช ืืืืื ืืื ืฉื ืืืืฆื ืืืืฃ ืขืืืื ืืืจืื ืืืืงืช ืืืืืื. ืืืื ืขื ืืืืืืช ืืืื ืืช ืืืขืืื ืืช ืจืืช
,ืืืืืฆืืืช ืืืืฃ ืืื ืืฉืจ ืืืื, ืืืฆืื, ืฉืืื ืื ืจืืืืื, ืฉืื ืื ืืืฆืงืื, ืืืื ืืืืื ...ืืื ืืืืฆืจื ืืื
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"ืกืคืจืืช ืืืงืฆืืขืืช"1964-2002
Br Med J (Clin Res Ed). 1981 J un 20;282(6281):2027-9
Rheumatoid arthritis and food: a case study.
Parke AL, Hughes GR.
Clinical and laboratory studies in a patient whose rheumatoid arthritis
appeared to be exacerbated by dairy produceโฆ
Acta Med Scand. 1972 Sep;192(3):231-9.
The primary cause of rheumatoid arthritis is an infection--the infectious agent exists in milk.
Svartz N.
Arthritis Rheum. 1986 Feb;29(2):220-6.
Food-induced (allergic) arthritis. Inflammatory arthritis exacerbated by milk.
Panush RS, Stroud RM, Webster EM.
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Ann Rheum Dis. 1988 J an;47(1):69-72.
Effect of dietary restrictions on disease activity in rheumatoid arthritis.
Beri D, Malaviya AN, Shandilya R, Singh RR. Department of Medicine, All India Institute of Medical Sciences, New Delhi.
โฆ patients with rheumatoid arthritis (RA) โฆ diet free from pulses, cereals,
milk, and non-vegetarian protein foods. Fourteen patients finally took part in the trial, 10 (71%) of whom showed significant clinical improvement. The study indicates that dietary factors may influence inflammatory response in RA.
Ann Rheum Dis. 1992 Mar;51(3):298-302.
Food intolerance in rheumatoid arthritis. I. A double blind, controlled trial of the clinical effects of elimination of milk allergens and azo dyes.
van de Laar MA, van der Korst J K. Department of Rheumatology, J an van Breemen Instituut and Academisch, Medisch Centrum, University of Amsterdam, The Netherlands.
One diet was allergen free, the other allergen restricted, containing only
lactoproteins and yellow dyes. Ninety four patientsโฆ Comparison between
baseline and subsequent periods showed only subjective improvements. No
differences were seen between the clinical effects of the two tested
diets
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2001ืื ืืืจืืช ืืฉื ืช ืืฆืขืืช ืืืืงืจ
ื ืืฉื ืืืืงืจ ืชืืื ืืชืืืืช ืืืงืจ ืจืืฉื
ื"ืจ ืืืื ืืืจื
ืจืืคื ืืืืจ, ืืืืงื ืคื ืืืืช ื', ืืคืจืืค' ืืืจืื ืืื ืืจืืจ, ืื ืื ืืืืงื ืคื ืืืืช ื' ,
ืื"ื ืืืืื ืกืื
ืจืคืืื ืคื ืืืืช
ืืฉืคืขื ืฉื ืืืืืช ืืืืืืช ืืืืืืงืื ืคืจืืืืืืืื
ืขื ืืชืคืชืืืช ืืื ืืืืช ืืืฆ'ื
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J Food Prot. 2002 J an;65(1):153-60.
Oral intake of Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 prevents collagen-induced arthritis in mice.
Kano H, Kaneko T, Kaminogawa S. Central Research Institute, Meiji Milk Products Co, Higashimurayama, Tokyo, J apan.
.. Study of effects of Lactobacillus sp. on the development of autoimmune
disease in mice with collagen-induced arthritis (CIA).
Oral intake of skimmed milk fermented with Lactobacillus delbrueckii
subsp. bulgaricus OLL1073R-1 markedly inhibit the development of CI A
in these mice.
The inhibitory effect of SM fermented OLL1102 or fresh SM was weaker than
that of SM/ OLL1073R-1.
The inhibitory effect of SM/ OLL1073R-1 is attributable not only to SM
components but also to OLL1073R-1 cells, their metabolites, or both.
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Postgrad Med J . 2002 J un;78(920):366-7.
Yoghurt biotherapy: contraindicated in immunosuppressed patients?
MacGregor G, Smith AJ , Thakker B, Kinsella J . Department of Respiratory Medicine, 2nd Floor OPD, Western General Hospital, Crewe Road South, Edinburgh EH4 2XU, Scotland, UK.
A fatal case of Lactobacillus rhamnosus septicaemia
after prolonged oral vancomycin for recalcitrant Clostridium difficile infection is
reported. The patient was immunosuppressed with cyclophosphamide and
steroids for Sjรถgren's syndrome. The administration of Lactobacillus spp as
"biotherapy" may be hazardous in such circumstances.
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The Preventive effect of Yogurt, LGG Yogurt, and LGG Bacteria on AA. Day -7
0
2
4
6
8
10
12
14
16
11 13 15 18 20 22 25 27 29 31 33 48
Day Following Induction
Art
hri
tis S
co
re
Water
Heat Killed LGG
Live LGG
Milk
Yogurt
Yogurt LGG
a
b
b
b
c
a
-7
Tx
0
Immunization
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ืืกืงื ืืช
ืืืืืจื "ืจืืื" ืืืืืืจื ืคืจืืืืืื โขืืขืืืื LGGืืืืื ืืืืงื
ืืืงืช ืืืคืจืงืื ืืื ืืขืช ืืืืืืื ืืืื ืืื ืืืื ืืกืืืืช
ืืืื LGGืืื ืืขืืงืจ ืืืืืงื โขืื ืืืืชืื - ืืชืืืกื ืืืืืช,
ืืืงืช ืืื ืืขืชืืขืืืื ืืืคืจืงืื ื ืืกืืืืช, ืืื ืืืื
ืคืืืช ืืขืืืื ืืื ืืื, ืื ืืืืืืืจืืื ืฉื ืืืงื
0
2
4
6
8
1 0
1 2
1 4
1 6
1 1 1 3 1 5 1 8 2 0 2 2 2 5 2 7 2 9 3 1 3 3 4 8
D a y F o l l o w i n g I n d u c t i o n
Art
hri
tis
Sco
re
W a t e r
H e a t K i l l e d L G G
L i v e L G G
M i l k
Y o g u r t
Y o g u r t L G G
a
bb
b
c
a
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The Therapeutic effect of Yogurt, LGG Yogurt, and LGG Bacteria on AA. Day +14
0
2
4
6
8
10
12
14
14 17 19 22 24 26 28 30 32 35 37 40 42 44
Day
Art
hri
tis S
co
re
W ater
LGG Killed
LGG Live
Milk
Emi LGG
Yogurt
ab
b
b
aa
a
Tx
0
Immunization
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ืืกืงื ืืช
LGGืืืืืจื "ืจืืื", ืืืืืจื ืืืืื โข ืืืื ืืชืจืืืฃ ืืืื, LGGืืืืืงืื ืืืืงืช ืืืคืจืงืื ืืืืคืืืืขืืืื
ื ืืกืืืืช, ืืืื ืืืื ืืื ืื.
ืืืืชืื ืืชืจืืืฃ ืืืื LGGืืืืงืื โขืืื ื ื ืืืืื ืืืฉืคืขืชื ืืืืืืจื
ืืืืืงืื LGG"ืจืืื", ืืืืืจื ืืืืื LGG ืืืื ืื ืื ืื ืืืืืืืืช
ืืืืืืช.
ืืื ืืขืืงืจ ืืื ื ืืขืื ืืืืคืื โขืืืืงืช ืืืคืจืงืื ื ืืกืืืืช.
0
2
4
6
8
1 0
1 2
1 4
1 4 1 7 1 9 2 2 2 4 2 6 2 8 3 0 3 2 3 5 3 7 4 0 4 2 4 4
D a y
Art
hri
tis
Sco
re
W a t e r
L G G K i l le d
L G G L iv e
M i lk
E m i L G G
Y o g u r t
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ืื ืขืืฉืื?
ืืืงืจ ืงืืื ื?โข
ืืืืืโข
ืืชืืจืื? โข
ืคืจืืืืืืืงื ืืืจืช โข
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The Preventive Effect of Probiotic Yogurt* on AA
0
2
4
6
8
10
12
12 15 18 21 24 27Days
Art
hri
tis
Sc
ore
WaterMilkProbioticYogurt
*Probiotic Yogurt:
1. Bacillus bifidum
2. Lactobacilus thermophylus
3. Acidophilus
a
a
b
b
7
Tx
0
Immunization
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ืืื ืขืืฉืื?
ืืืงืจ ืงืืื ื?โข
ืืืืื โข
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ืืกืงื ืืช
ืืืงืช ืืื ืืขืช ืืขืืืื ืืื LGGืืืืืจื "ืจืืื" ืืืืืืจื โขืืืื ืืืื ืืื ืืืืืืคืจืงืื ื ืืกืืืืช
ืืืื ืื ืืืืชืื - ืืชืืืกื ืืืืืช, LGGืืื ืืขืืงืจ ืืืืืงื โข ืืืงืช ืืืคืจืงืื ื ืืกืืืืช, ืืื ืืืื ืืื ืืืืืื ืืขืชืืขืืืื
ืืืื ืืชืจืืืฃ ืืืื, LGG ืืืืืงืื LGGืืืืืจื "ืจืืื", ืืืืืจื โข ืืืืงืช ืืืคืจืงืื ื ืืกืืืืช, ืืืื ืืืื ืืื ืื.ืืืืคืืืืขืืืื
ืืืืชืื ืืชืจืืืฃ ืืืื ืืื ื ื ืืืืื LGGืืืืงืื โข ืืืืืงืื LGGืืืฉืคืขืชื ืืืืืืจื "ืจืืื", ืืืืืจื ืืืืื
LGG.ืืืื ืื ืื ืื ืืืืืืืืช ืืืืืืช
ืืืืงืช ืืืคืจืงืื ื ืืกืืืืช.ืืืืคืืืืื ืืขืืงืจ ืืื ื ืืขืื โข
ืืืงืช ืืื ืืขืช ืืืืืจื ืคืจืืืืืื ืืืืื ืืืืงืื ืฉืื ืื ืืขืื โขืืืคืจืงืื ื ืืกืืืืช.
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ืกืื ืื ืืืืืืจื
Supernatant
Filtrate
0.22 microM
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The Preventive Effect of LGG Yogurt Filtered Fractions on AA
0
1
2
3
4
5
6
7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39
Days After Immunization
AA
Sco
re
AA
AA Filter
AA Supernatant
a
a
b
0
Immunization
Tx
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Filtered
Precipitate
Supernatant
Saturated Amonium sulfate
ืืฉืงืขืช ืืืืื
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Dialysis x 3, 1:500 Vol/Vol, 24 hr, Pores 8 Kdalton
Precipitate Supernatante
pH=5
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The Preventive Effect of LGG Yogurt Protein Precipitate on AA
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Immunization
Tx
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2008ืืืืฆืืช ืงืืื ืืืช
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I ssue: 1 February 2008 Supplement of: Volume 46, Number 3, 1 February 2008, pp. 327-e30
Clinical I ndications for Probiotics: An Overview
B. R. Goldin and S. L. Gorbach
Tufts University School of Medicine, Boston, Massachusetts
Probiotic bacteria are used to treat or prevent a broad range of human diseases, conditions, and syndromes. In addition, there are areas
of medical use that have been proposed for future probiotic applications. Randomized double blind studies have provided evidence of
probiotic effectiveness for the treatment and prevention of acute diarrhea and antibiotic induced diarrhea, as well as for the prevention
of cow milkโ induced food allergy in infants and young children. Research studies have also provided evidence of effectiveness for the
prevention of travelerโs diarrhea, relapsing Clostridium difficileโ induced colitis, and urinary tract infections. There are also studies
indicating that probiotics may be useful for prevention of respiratory infections in children, dental caries, irritable bowel syndrome, and
inflammatory bowel disease. Areas of future interest for the application of
probiotics include colon and bladder cancers, diabetes, and rheumatoid arthritis. The probiotics with
the greatest number of proven benefits are Lactobacillus rhamnosus strain GG and Saccharomyces boulardii.
Reprints or correspondence: Dr. Barry R. Goldin, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111 ([email protected]).
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ืชืืื