V. English Speaking Session

El SINCXE QLL ANALYSIS OF LWADYKlNlN-INDULWI CALCIlJll m%

IN HUMAN KERATINM?ITEZ

Y.Sato’ H.Takagi’ W.Seishia’ S.Mori* Y.Nozaw”

Uepartments of Deratolagy’ and Eiachemistry**.Cifu Unlrerrity

School af lMicine.Gifu

Recent studies have rerealed thai protein kinase G (PKC) is

associated with the aitogen-anduced responses in various trps

of cells.such as siss 373 cells and leukemia cells. Horerer. it

remains unclear about the effect of PKC on the calcium resp~*Pe

in human keratinocytes

In this study, we ermind the effect of pborbol 12-wrist& 13 acetate (PlfA,PKC activator) on bradykinin @K)-induced

calcium responses in cultured human keratinocytes by using fura-

2 and fluorescent sideomicroscapy. More than 90% of the cells

respansed transiently ta BK. Pretreatment of the cells with PWA resulted in shortening of the tesponse time to BK. These

results suggetest that PKC aghi be involved in intra-cellular

calcium resrxvnse to BK.

E3 A Study of Prevention Assay for Microcomedone Formation

by Red Ginseng Water Extract and Saponin.

Dept. of Dermatology Chungnam National Univ. Sch. of Med.

Beomjin Seong, Haeyung Kim, Jeunghoon Lee, Jangkyu Park

We performed microcomedone prevention assay using 50% sapo-

nin and 50% water Extract of Red Ginseng(=WERG) as Lest mate-

rials and Oleic acid, comedogenic agent, as control.

We found that Saponin and WERG prevented the microcomedone

in 76% and 318, respectively, comparing with oleic acid only

application group as concral. And the mean number and size

af prominent comedone, defined comedone above 0.0351~1 in

diameter, were decreased and small in Saponin applicarion

group statistically signficantly comparing with control group

E5 THE APPLICATION OF THE ELECTRON MICROSCOPIC TECHNIQUE IN THE INVESTIGATION OF DNA

MASAKO UUONO. MAKOTO HORL. RYOZI HIROSE. MASAO YAYAOA. TAKASI KOIUE. HIKOTARO YOSHIOA. Department of Dermatology. Nagasaki Univ. School of Medicine. Nagasaki, Japan.

Morphological studies on DNA are reported infrequently. Some findings which could be elucidated only by the electron micro- scopic observation will be presented as follows: @ morpho- logical changes of DNA by UV irradiation, @ the location of photoproducts on DNA strands. @ confirmation of ligated and digested DNA with enzymes and @ distinction between DNA and other substance. when they could not be distinguished by gel electrophoresis. 0 morphological observation of DNA extracted from skin tumors.

UV irradiated human DNA was bizzarly shaped and its density tended to decrease ONA from skcn cancers also showed frizzeld lines These peculrar DNA shapes might be caused by a distortion in three-dimensional structure. Though the DNA treated with an enzyme showed expected results gel electrophoreticallu. undetermined substance and DNA of unexpected lengths were sometimes observed electron microscopically. Thus, rnxroscopic studies may provide much unique information.

E4 THE APPLICATION OF PCR IN DIAGNOSING THE

SKIN DISEASE

KOICHI YOKOTA. HITOSHI KOBAYASHI, AKIRA OHKAWARA. MITSUO NARITA. TAKEHIRO TOGASHI De~rtment of Dermatology and Pediatrics, Hokkaido University School of Medicine, Sapporo

The polymerase chain reaction (PCR) was recently developed

for in vitro amplification of the DNA and RNA, and has

become a useful tool in the detectron of a certain target

sequences. We have utilized this technique for HTLV- I and

mycobacterium tuberculosis. The DNAs were extracted from

formalin-fixed and paraffin-embedded tissues of lymphoma

patients and suspicious Tbc patients. The specific sequences

of both organisms were successfully amplified by the PCR l suggesting the usefulness of this technique in diagnosing swne

skin diseases.

E6 FLOW CYTOMETRIC ANALYSIS OF THY-l LOW POSITIVE CELLS

ON INDUCTION PHASE OF CONTACT HYPERSENSITIVITY

HIDEKI MORITA, TAKAO MIYAZAKI, MIKA HOI% NVRIKO YAMASHITA, MASARU NATSUAKI, YOKIO KITANO.

AND SEICHIRO SAGAMI Department of Dermatology, Hyogo College of Medicine, Nishinomiya.

Hyogo 663

Phenotypic analysis of lymph node cells using flow cytometry was

performed on the Induction phase of contact hypersensitivity reaction

(CHR). Thy-1 antigen weakly positive cells (Thy-l low) appeared in the regional lymph node cells at 3 days and disappeared at 14 days

after sensltlzation. Two color analysis of the regional lymph node cells using anti-thy-1 antlbody and anti-L3T4 antibody suggested that

the Thy-l low was one of the groups belonging to the L3T4 negative

cell group. We considered that Thy-l low may play an important role

in contact sensitization during the induction phase.