th1_gene pyramiding to improve green super rice by molecular marker-assisted selection
DESCRIPTION
3rd Africa Rice Congress Theme 1: climate resilient rice Mini symposium: Enhancing rice yield potential Author: Yuqing HeTRANSCRIPT
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Gene Pyramiding to Improve Green Super Rice by Molecular Marker-assisted Selection
Yuqing He
National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural UniversityWuhan, ChinaEmail: [email protected]
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The Problems of hybrid rice in China
• Lose of disease resistance (blast, bacterial blight)
• Susceptible to insect pest (yellow stem borer and brown planthopper)
• Cooking and eating quality• Ever increasing demands for increase yield
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Strategies for developing Green Super Rice
Green Super Rice should possess resistances to
multiple insects and diseases, high nutrient
efficiency, and drought resistance, promising
to greatly reduce the consumption of
pesticides, chemical fertilizers, and water.
Zhang, PNAS, 2007, 16, 104 (42): 16402–16409
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Bt gene to improve hybrid rice with stem borer and leaffolder resistance
Tu, J.et al. Nature Biotech, 2000,18:1101-1104
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Bacterial Blight resistance to improve hybrid rice
G14652.0
RG11091.5
C50
2.8
C1331.0C10030.8C1890.8RG103212483.0
AB9
3.0
CDO534
Xa21
Chr. 11
Xa7
M5
4.0
STSP3
0.9
AFLP1415
Marker from IRRI
Chr. 6
Chen et al. Crop Science, 2000, 40: 239-244
Zhang et al, Plant Breed, 2006, 125, 600-605
Xa23
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Genotype Genes No. GX325 Mean KS-1-21 Mean
xa21xa21xa7xa7 0 A 19.58 A 22.071
xa21xa21Xa7xa7 1 B 12.38 B 4.20
Xa21xa21xa7xa7 1 C 6.47 C 7.96
Xa21Xa21xa7xa7 2 D 4.84 D 5.05
xa21xa21Xa7Xa7 2 D 4.31 C 7.16
Xa21Xa21Xa7xa7 3 E 2.67 D E 3.64
Xa21xa21Xa7xa7 2 E 2.31 E 3.33
Xa21xa21Xa7Xa7 3 E 2.10 E 3.09
Xa21Xa21Xa7Xa7 4 E 1.61 E 2.23
Comparison of bacterial resistance to GX325 and KS-1-21 as shown by lesion length
among nine genotypes of an F2 population using Tukey-Kramer HSD test
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MH63(Xa21) and MH63(CK)
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The locations of Pi-1 and Pi-2
Y2668LAY6854L
R251
RM224
R543a
RZ536
4.5
5.1
2.2
6.8
9.7
5.1
BC1F2 of
Zhenshan 97/BL5
Chr 11
Pi-1
C235
RG64
AP22
RG445
RZ632(R2123)
1.2
0.3
1.5
0.4
Pi-20.9
Chr 6
F5 (CO39/C101A51) RILs
Pi9, Pigm, R6
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NILs gene Infection strains
Resistance frequency (%)
C101LAC Pi1 13 82.7C101A51 Pi2 11 85.3C104PKT Pi3 57 24.0
BL1 Pi1+Pi3 8 89.3BL2 Pi1+Pi3 7 90.7BL5 Pi1+Pi3 5 93.3BL3 Pi2+Pi3 4 94.7BL4 Pi2+Pi3 5 93.3BL6 Pi1+Pi2+Pi3 2 97.3
CO39 ( ck) no 75 0
Zhenshan97 unknown 46 38.7
Reaction of Pyricularia grisea isolates on the differential near isogenic lines (NILs) (Liu et al, 2003)
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Blast natural identify area
Yuan’an, Hubei (Altitude:550m)
Enshi, Hubei
( Altitude:600m )
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Fig. 2 The distribution of blast
resistance scores of the improved
versions of Jin 23B and their hybrids
compared with control varieties. a the
leaf blast resistance scores of the
improved Jin 23B, hybrids, and control
at tillering stage. b the leaf blast
resistance scores of the improved Jin
23B, hybrids and control at heading
stage. c the neck blast resistance scores
of the improved Jin 23B, hybrids and
control at maturity stage.
Jiang et al, Mol Breed: 2012
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Bph14
QBph3
Bph11(t)RM514
SG1
15.0
SG6
9.0
RM570
8.0
IN762
10.0
J14-87.0
J14-127.0
Chr 3RM8213
RM164342.2
RM261
19.1
HJ163.6
IN1560.7
J23/MS52.2
HJ28
10.1
RM5953
11.5
Bph12
Bph17
Bph15
QBph4.1
QBph4.2
Bph20
Chr 4aRM307
HJ22
20.6
RM167172.9
RM16820
29.3
RM168536.1
J6-4
21.2
RM119
1.0
J6-100.9
RM252
26.9
bph16
Bph27
Bph6
Bph24
Chr 4b
RM313
RM463
12.5
J18-15
5.2
J18-4
0.2
IN187
2.1
HJ12
2
RM3331
4.2
HJ9
4
RM28625
6.6
RM2704.7
bph2
Bph10
Bph18
Bph9
Bph21
Bph1
Chr 12
BPH resistance genes
Hu et al, Mol Breed (2012) 29:61–69Hu et al, PMS, Pest Manag Sci (2013) 69: 802–808
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0
1
2
3
4
5
6
7
8
9
6303
S/93
11(1
4/15
/18)
6303
S/93
11(1
4/15
)
6303
S/93
11(1
4/18
)
6303
S/93
11(1
5/18
)
6303
S/93
11(1
4)
6303
S/93
11(1
5)
6303
S/93
11(1
8)
6303
S/93
11
GZ
63S/
9311
(14/
15/1
8)
GZ
63S/
9311
(14/
15)
GZ
63S/
9311
(14/
18)
GZ
63S/
9311
(15/
18)
GZ
63S/
9311
(14)
GZ
63S/
9311
(15)
GZ
63S/
9311
(18)
GZ
63S/
9311
Y58
S/93
11(1
4/15
/18)
Y58
S/93
11(1
4/15
)
Y58
S/93
11(1
4/18
)
Y58
S/93
11(1
5/18
)
Y58
S/93
11(1
4)
Y58
S/93
11(1
5)
Y58
S/93
11(1
8)
Y58
S/93
11
B5(
14/1
5)
Hu7
(18)
TN
1
Res
ista
nce
Scor
e
Hybrids
The resistance score of pyramided two-line hybrids
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21 days
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BPH resistance in field experiment (4 hectares)(2010, Tongcheng, Hubei)
GZ63S/9311(ck) GZ63S/9311(Bph14/Bph15)
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GZ63S/9311(ck) GZ63S/9311(Bph14/Bph15)
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Whole genome selection breeding
R6: blast resistance gene60K SNPs Background selection
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Kongyu 131(Pi2)
Pi2
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Ph D and postgraduate: Hu JieYuan LiGao Guanjun Tu JumingChen Sheng Jiang Gonghao Zhang Jinfeng Yang Zixian Li Xin Liu Shiping Wu Changjun
Acknowledgement
StaffRice group
Collaboration:
IRRI
SAGC
CAAS
Wuhan University
Cornell University
Supported: 863 plan; National Natural Science Foundation
in China and Bill and Melinda Gates Foundation
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Thank You!