tf_template_word_mac_2011 - s3-eu-west web viewfigure s3. (a) cumulant diameter and pdi of template...
TRANSCRIPT
Supporting Information
Multilayered Polyion Complexes with Dissolvable Silica Layer
Covered by Controlling Densities of cRGD-conjugated PEG Chains for
Cancer-targeted siRNA Delivery
Mitsuru Naitoa, Ryota Azumab, Hiroyasu Takemotoc, Mao Horid, Naoto
Yoshinagad, Shigehito Osawae, Rimpei Kamegawab, Hyun Jin Kima,
Takehiko Ishiid, Nobuhiro Nishiyamac, Kanjiro Miyataa,b*, Kazunori
Kataokae,f**
aCenter for Disease Biology and Integrative Medicine, Graduate School of Medicine,
The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
bDepartment of Materials Engineering, Graduate School of Engineering, The University
of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
cLaboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo
Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan
dDepartment of Bioengineering, Graduate School of Engineering, The University of
Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
eInnovation Center of NanoMedicine, Institute of Industry Promotion-KAWASAKI, 3-
25-14 Tonomachi, Kawasaki-ku, Kawasaki 210-0821, Japan
fPolicy Alternatives Research Institute, The University of Tokyo, 7-3-1 Hongo, Bunkyo-
ku, Tokyo 113-0033, Japan
Corresponding author.
*Email: [email protected]
**Email: [email protected]
1
Scheme S1. Synthesis scheme of cRGD-PEG-PAsp(TEP).
2
Figure S1. 1H NMR spectrum of PAsp(TEP) (polymer concentration: 10 mg/mL,
solvent: D2O, temperature: 80 °C).
3
Figure S2. 1H NMR spectrum of cRGD-PEG-PAsp(TEP) (polymer concentration: 10
mg/mL, solvent: D2O, temperature: 80 °C).
4
Figure S3. (a) Cumulant diameter and PDI of template PICs prepared at varying N/P
ratios. (b) Cumulant diameter and PDI of sPICs prepared at varying concentrations of
sodium silicate. (c) Cumulant diameter of mPICs prepared at varying concentrations of
cRGD-PEG-PAsp(TEP).
Figure S4. TEM images of (a) template PIC, (b) sPIC-7.5, (c) sPIC-10, and (d) sPIC-15
(Scale bar: 200 nm).
5
Figure S5. STEM-EDS image of sPIC-15. Cross marks indicate the points where atomic
mass ratios were analyzed (Scale bar: 100 nm).
Figure S6. Intensity-weighted size distribution histograms of a series of mPICs
measured by DLS.
6
Figure S7. (a) Agarose gel retardation analysis of a series of PICs. (b) Encapsulation
efficiency of siRNA in each PIC sample, determined by densitometry of the gel.
Figure S8. Viability of cultured HeLa-Luc cells after 48 h incubation with (+)mPICs
with siCon (siRNA concentration: 100 nM). The cell viabilities were determined using
CCK-8. Results are expressed as mean ± SD (n = 5).
7