Título da Prática.Pedro Souza, Roseli Maria, Ademir SilvaDepartamento de Química da Universidade xxxxxxxxxxxxxxxx

Palavras chave – Complexos, Química, xxxxxxxx, xxxxxxx. Resumo: Descrever em poucas palavras o que foi feito e qual resultado obtido.

INTRODUÇÃO

Binuclear Metallohidrolases are responsible for the catalysis of numerous reactions in biological systems. Several metabolic steps have the hydro-lysis of peptides or phosphate esters are involved in these mechanistic pathways. This class of en-zymes has been studied, and present a diversity of structures, active sites and metal ions present in their structures.

Due to their functional diversity in these biolo-gical systems, these enzymes are commonly used as targets for developing drugs against various types of human disorders. Following this same idea, many coordination compounds have been developed to mimic these enzymes seeking to obtain compounds with potential medicinal ap-plication.

Vários complexos heterobinucleares de FeZn, GaZn e Homobinucleares de CuCu e ZnZn tem sido estudados como fosfodiesterases e nucleases

In this study, we report the cytotoxic and photocytotoxic activity in cancer cells of two het-erobinuclear complexes. Their antibacterial effect in four bacterial strains was also evaluated.

PARTE EXPERIMENTALMateriais e Métodos. All starting materials were purchased from Aldrich, Acros, or Merck. Measures to determine the percentages of car-bon, hydrogen and nitrogen were performed on an elemental analyzer CHNS - Carlo Erba Model E-1110. The spectra in the infrared region were obtained on a Varian spectrophotometer in the region 4000-500 cm-1. Electrospray ionization mass spectrometry (ESI-MS) of 1 and 2 dissolved in an ultrapure acetonitrile solution (500 nM) was performed using an amaZon X Ion Trap MS instru-ment (Bruker Daltonics) with an ion spray source

using electrospray ionization in positive-ion mode. The ion source condition was an ion spray voltage of 4500 V. Nitrogen was used as the nebulizing gas (20 psi) and curtain gas (10 psi). The samples were directly infused into the mass spectrometer at a flow rate of 180 μL/h. The scan range was m/z 200–2000. The simulated Spectrum was cal-culated using the Mmass software [REF] and [REF]. Potentiometric studies of 1 and 2 were carried out in an acetonitrile/water mixture (1:1, v/v) with a Metrom research pH meter fitted with blue-glass and Ag/AgCl reference electrodes. Equilibrium measurements were performed in a thermostated cell, purged with argon, containing 50.00 mL of the acetonitrile/water (1:1) solution and 0.03 mmol of the complex. Computations of the triplicate results were carried out with the BEST7 program, and species diagrams were ob-tained with the SPE and SPEPLOT programs [ref].

Síntese. The unsymmetrical ligand H2bpbpmp was prepared and characterized according to methods described in the literature [ref]. Complex 1 and 2 was prepared by the same procedure, only substituting the corresponding zinc salt by copper salt. To a stirring solution of MeOH (50 mL) containing 0.54 g (1.0 mmol) of H2L at 25 oC, were added in the following sequence: 0.37 g (0.5 mmol) of Zn(ClO4)2.6H2O, 0.37 g (0.5 mmol) of Al(NO3)3.9H2O in 15 mL of MeOH and 1.5 mmol of NaOH in 6.5 mL of water. After standing the solution for 48 h, suitable crystals for X-ray ana-lysis were obtained. Yield: 90%. Anal. Calcd for C34H42Cl2FeN5O15Zn, fw = 952.85: C, 42.85; H, 4.44; N, 7.34. Found: C, 42.86; H, 4.48; N, 7.34.

RESULTADOS E DISCUSSÃO

Resultados. Both complexes are belonging to the monoclinic crystal system with space group P21 / c. The complexes shows a non-symmetrical

arrangement consisting of a complex cation [AlIIIZnII(bpbpmp)(µ-OH)(H2O)], two perchlorate anions as counter-ion and three molecules of wa-ter of crystallization. A visual representation of the complex cation generated by the program ORTEP 50 is shown in Figure 01 with their crystal-lographic data and key lengths and bond angles are shown in Supplementary Material.

Figure 1. Estrutura do complex 1.

ABREVIAÇÕESCCR2, CC chemokine receptor 2; CCL2, CC chemokine ligand 2; CCR5, CC chemokine receptor 5; TLC, thin layer chromatography.

REFERÊNCIAS(Word Style "TF_References_Section"). References are

placed at the end of the manuscript. Authors are re-sponsible for the accuracy and completeness of all references. Examples of the recommended formats for the various reference types can be found at http://pub-s.acs.org/page/4authors/index.html. Detailed informa-tion on reference style can be found in The ACS Style Guide, available from Oxford Press.

Guia

Introdução – devem conter conteúdo referente ao experiment (fundamentos).

Parte Experimental – Deve conter apenas a de-scrição do metrial utilizado (por extenso) e os procedimentos realizados (sem explicações).

Resultados e discussão – apresentar todas as jus-tificativas para os resultados obtidos.

Lista de abreviação

Referências – as refências listadas no fim do doc-umento devem estar indicadas por ordem numérica na sequência em que foram citadas ao longo de todo trabalho.

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