Technology of Engineering Food

The common between...

floundertomato

Tomato has an engineered gene from the flounder

Antifreezing gene

Survey

- What is biotechnology?

- What is DNA?

- What kind of mechanisms do exist?

- What is the best method?

Biotechnology- What is Biotechnology?

- What is DNA?

- What kind of mechanisms do exist?

- What is the best method?

DNA

Shortening of: deoxiribonucleic acid

-Segments of DNA are called genes-Four bases(Guanin, Cytosin, Adenin, Thymin)-Bases are connect with sugar and phosphat Double-helix-structure (like a spiral staircase)

- What is Biotechnology?

- What is DNA?

- What kind of mechanisms do exist?

- What is the best method?

Mechanisms- What is Biotechnology?

- What is DNA?

- What kind of mechanisms do

exist?

- What is the best method?

Biologically Mechanically

Vector: Bacterium

Vector: Virus

Injection

Biolistic

Protoplast Transformation

Agrobacterium Tumefaciens

Virus

Protoplast Transformation

- Taking a cell from an organism

- Dissolving of the cellwall by enzymes

Protoplast is left over

- Adding the new DNA

- Cellwall has to recover in a growth media

Injection

Injecting new DNA with a needle

into the nucleus of a cell.

Biolistic

1. Small beads coated with DNA are load in a gene gun.

2. Shooting the ball into the cell nucleus

3. It cuts the DNA section of the cell and „glues“ the desired DNA into it.

Marker Genes

To find out if the transformation has been succesfull

scientists intodruce a marker gene to the host.

Cells which have been transformed will grow in a media

while the other cells will die.

Best Method- What is Biotechnology?

- What is DNA?

- What kind of mechanisms do exist?

-What is the best method?

Method Pros ConsVector:

Bacteria, Virus

Natural process Parts from the bacteria or virus DNA are insert

too

Protoplast

transformation

Only the desiered DNA is transferred

Cellwalls have been damaged

Injection Only the desiered DNA is transferred

Maybe damaging the cellwall with the needle;

High rate of failure

Biolistic Nothing special Foreign things like titanium or gold are also

insert

Isn´t it fantastic what the scientists can do with their

technology?

But what will the future bring?

Do scientists know their limits?