TARGETED MAPPING OF LOCI CONFERRING RESISTANCE TO YELLOW RUST IN PAVON-76/ BORLAUG RIL POPULATION

IWSR Symposium ICARDA

Abdulqader Jighly;Yaljarouka A; Nazari K; Tahir I; Abdalla O; Ogbonnaya FC

ICARDA

OUTLINES

Introduction Serve epidemic 2009-10YR genes summaryYR genes – 2010

Aim of the study Study Plan Plant Material Progress so far Summary

INTRODUCTION Stripe rust, caused by Puccinia striiformis,

is one of the most globally important fungal diseases of wheat that cause significant annual yield losses.

In 2009-10, the breakdown of Yr27 has caused significant yield losses in Syria, Turkey, Iran, Uzbekistan, Morocco, Ethiopia, and Kenya, threatening the food security and livelihood of resource poor farmers (Hodson and Nazari 2010).

http://www.globalrust.org/traction?type=single&proj=Pathogen&sort=2&stickyparams=sort&rec=206

2009-10 STRIPE RUST SERVE EPIDEMIC IN SYRIA

Farmars field Idleb – Joubas

OPTIONS AVAILABLE FOR THE FARMERS

1. Fungicide

2. Disease Management

3.Using resistance varieties

Chromosome

GenesChromo.

CountGenome Count

1A YrDa1 1

1518.98%

2A Yr1, Yr8, Yr17, Yr32 43A YrTr2 14A YrHVII, YrMin, YrND 35A Yr34, Yr48 26A Yr42, YrD,YrDru2,YrH46 47A - 0

1BYr9, Yr10, Yr15, Yr21, Yr24, Yr26, Yr29, YrC143, YrAlp, YrH52, YrExp1, YrR212, YrCN17

13

4759.5%

2BYr5, Yr7, Yr27, Yr31, Yr41, Yr43, Yr44, YrCn19, YrZak, YrV23, YrSte, YrP81, YrS2199, YrSP

14

3B Yr4, Yr30, YrS, YrSte2, Yrns-B1 54B YrCle, YrMor, YrYam 35B Yr3, Yr19, Yr47, YrDru, YrExp2 56B Yr35, Yr36 27B Yr2,Yr6, Yr39, YrC591, YrZH84 51D Yr25 1

1721.52%

2D Yr16, Yr37, YrCK 33D Yr45 14D Yr22,Yr28,Yr46,YrAS2388 45D Yr40,YrDa2 26D Yr20, Yr23, YrTye, YrTr1 47D Yr18,Yr33 2Total   79 100%

SUMMARY OF YR GENES

DESIGNATED GENES FOR RESISTANCE TO STRIPE RUST 2010

Gene Chromo. Sources Linked

Markers Ref.

Yr4 3BS Avalon, Bolac, Emu S, Rubric AUS33333 (Bread wheat) Xcfb3530 Bansal et al.

2010

Yr43 2BL PI 591045, Lolo, many IDO377s derivatives (Spring Wheat)

Xwgp110, Xwgp113

Cheng and Chen 2009

Yr44 2BL PI 607839 (Spring Wheat) Xwgp100 Sui et al. 2009

Yr45 3DL PI 181434 (Spring Wheat) Xwp115, Xwp118

Li et al. 2010

Yr46 4D PI 250413 (common wheat) Xcfd71, Xbarc98

Herrera-Foessel et al. 2009

Yr47 5BS AUS28183 (common wheat)5 ± 2 cM

proximal to Lr52

Bansal U et al. 2011

Yr48 5AL PI 610750 (Bread wheat) Xwms291 Dubcovsky 2010

PREVIOUS WORK

The Pavon-76 variety (spring wheat) has been mapped before by (Williams et al. 2006) in the cross Pavon-76/Avocet with AFLP and SSR markers.

Based on the previous study, three locations (1BL, 3BS and 6BS) showed significant link to stripe rust resistance in Pavon-76 with some AFLP markers and only 1 SSR marker on 1BL.

The source of resistance (Pavon-76) showed a very good resistance level for the new stripe rust race.

AIM OF THE STUDY

Developing molecular markers which are more closely linked to those genomic locations and more useful for breeding programs and marker assisted selection than AFLP markers.

Discovering more genomic locations that linked to stripe rust resistance in this important gene pool.

THE STUDY PLAN

Field Scoring for two years in Tel-hadya. Bulk segregant analysis using molecular

markers with known link to different YR genes

Applying different kinds of molecular markers to construct a high resolution linkage map which are: SSR CAPs SNPs (based on NSF wheat SNP project) RGA EST-SSR

THE STUDY PLAN

The following link contains 5425 designed EST-SSR primers for wheat.

We search for RGA motifs with more than 70% identity in this file. The ESTs that contain high density of RGA motifs have been chosen to be requested.

CA707339http://wheat.pw.usda.gov/ITMI/EST-SSR/LaRota/table3_est-ssr%20designed%20primers.xls

PLANT MATERIALSStripe rust resistance has been

studied on 301 recombinant inbred lines (F7) of the cross Pavon-76/Borloug in 2009-10.

The source of resistance (Pavon-76) showed a very good resistance level

RESULTS SO FAR

PHENOTYPIC VARIATION 2009-10

0-4

5-9

10

-14

15

-19

20

-24

25

-29

30

-34

35

-39

40

-44

45

-49

50

-54

55

-59

60

-64

65

-70

71

-75

76

-80

0

5

10

15

20

25

R M S

33.67% 31.33% 35%

%

The score is according to CIMMYT coefficient

0-4 5-9 10-24 25-44 45-800

5

10

15

20

25

30

35

%

PHENOTYPIC VARIATION 2009-10

R M S

33.67% 31.33% 35%

PLANTING THE POPULATION AT ICARDA FIELD 2010-2011

Spreader

BULK DESCRIPTION The DNA of at least five lines that showed

similar resistant classification were used for bulk segregant analysis as follows: 10 MR 15 MR 20 MR 20 M * S

76 SSR (simple sequence repeat) and RGA (resistance gene analogue) markers should be applied on those bulked lines, 30 of them have been applied and the others have been requested.

BULK RESULTS Two markers on chromosome 1BL were

polymorphic between the bulks . Each marker seems to be linked to a

different gene because the first one –Xwmc631– showed coupling phase with the source of resistance (Pavon-76) and the other one –Xbarc181– showed a repulsion phase with the same parent.

The 1BL linkage group indicate that.

UP TO DATE

cfd48

barc181

barc137

gwm273wmc631

32.9

17.3

10.9

3.9

1BL

barc55

gwm148

16.8

2BS

0 2 4 6 8

cfd48

barc181

barc137

gwm273wmc631

LOD

0 5 10

Exp

Gro

up

1B

LU

nm

ap

pe

d

Le

ge

nd

« L

OD

» %

Exp

l.

Borl

Pavon

-76 *

S20

MR

20

M15

M

R10

M

R

Borl

Pavon

-76 *

S20

MR

20

M15

M

R10

M

R

UP TO DATE We also get a single markers on

chromosome 6BS –Xwmc487– which linked to stripe rust resistance but we weren’t able to construct a linkage group because of the lack of polymorphic markers.

The R2 value for this region was about 5%, this may increase if we add more markers.

380 SSR markers has been screened on the parental lines:92 markers of the whole set showed

polymorphic alleles.15 markers of those 92 polymorphic

ones have been applied on the whole population.

7 markers of those 15 have been mapped on two locations 1BL and 2BS

UP TO DATE

SUMMARY Pavon-76 showed a good level of

resistance to the new race of stripe rust.

Pavon-76/Borloug RIL population has been planted again in 2010-11.

Two QTLs on chromosome 1BL and one on 6BS has been detected so far based on some SSR markers.

Different molecular markers (EST-SSR, RGA, STS) are being applied on the population to identify markers for MAS.

THANK YOU