taking a deep look: modern microscopy technologies to optimize the design and functionality of...

9
Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine by M. Vielreicher, S. Schürmann, R. Detsch, M. A. Schmidt, A. Buttgereit, A. Boccaccini, and O. Friedrich Interface Volume 10(86):20130263 September 6, 2013 ©2013 by The Royal Society

Upload: maria-farmer

Post on 13-Jan-2016

215 views

Category:

Documents


1 download

TRANSCRIPT

Page 1: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible

scaffolds for tissue engineering in regenerative medicine

by M. Vielreicher, S. Schürmann, R. Detsch, M. A. Schmidt, A. Buttgereit, A. Boccaccini, and O. Friedrich

InterfaceVolume 10(86):20130263

September 6, 2013

©2013 by The Royal Society

Page 2: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

MRI, µCT and OCT imaging.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 3: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

White light, fluorescence and electron microscopy applications in TE. (a) White light images of tissue slices after histological staining.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 4: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

Multiphoton microscopy: principle, micrometre-scale excitation and tissue penetration.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 5: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

Sources of intra- and extracellular autofluorescence.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 6: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

Two-photon AF images of cells in different scaffold designs.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 7: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

Two-photon excitation and imaging of selected fluorescent dyes.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 8: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

SHG imaging of native tissue and various biomaterials.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society

Page 9: Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative

Timeline and SHG imaging of collagen I production.

M. Vielreicher et al. J. R. Soc. Interface 2013;10:20130263

©2013 by The Royal Society