table of contents el… · handbook naktuinbouw elite ornamental crops back to table of contents...

Handbook Naktuinbouw Elite Ornamental Crops Version: 19, 01-01-2021 Page 1 of 195

Table of contents Table of contents ............................................................................................................................. 1 Regulations on the use and control of the Collective Naktuinbouw Quality Brand ‘Elite’ ............. 2 Participation agreement for Naktuinbouw Elite collective trademark ........................................... 7 Certification regulations Naktuinbouw Elite Ornamental Crops ..................................................... 8 Appendix I Specific requirements for identity, trueness to variety and trueness to type for

propagation material of ornamental crops to be accepted for certification ................................ 17 Appendix II Requirements for the testing for pathogens of propagation material of ornamental

crops .............................................................................................................................................. 19 Appendix II.A Control by participant ............................................................................................. 19 Appendix II.A Control participants protocols ................................................................................ 23 Appendix II.B. Control by Naktuinbouw ........................................................................................ 83

Appendix II.B Control by Naktuinbouw protocol .......................................................................... 88 Appendix II.C Reduction of control sampling .............................................................................. 126 Appendix II.D List of crops (genus) .............................................................................................. 127 Appendix III Additional health regulations and requirements for the certification of propagation

material for ornamental crops .................................................................................................... 149 Appendix IV Summary pathogens ............................................................................................... 150 Appendix V Certificate ................................................................................................................. 155 Appendix VI Naktuinbouw Module Quality Management System requirements and sampling

requirements ............................................................................................................................... 156 Participation protocol in Naktuinbouw Elite Ornamental Crops for “breeders” ........................ 167 Participation protocol in Naktuinbouw Elite Ornamental Crops for “traders” ........................... 169 Non Conformity Sheet ................................................................................................................. 171 Participants Naktuinbouw Elite Ornamental Crops .................................................................... 172 ASLN authorized laboratories ...................................................................................................... 173 Contacts Naktuinbouw Elite Ornamental Crops ......................................................................... 174 Organizations declared equivalent by Naktuinbouw .................................................................. 175 Instruction use of the logo by third parties ................................................................................. 176 Instruction for the use of Naktuinbouw Elite indication on the participant’s own delivery sheet

and/or label ................................................................................................................................. 177 Instruction sampling plant material and water ........................................................................... 178 Instruction combining samples plant material and water .......................................................... 181 Instruction disinfecting water ..................................................................................................... 186 Procedure approach ‘new’ pathogen in the framework of Naktuinbouw Elite Ornamental

Crops ............................................................................................................................................ 187 Statement plant breeders right ................................................................................................... 189 Guideline external communication within the framework of Naktuinbouw Elite Ornamental

Crops ............................................................................................................................................ 190 Definitions / references ............................................................................................................... 191 Revision history (requirements 2021 compared to requirements 2020) ................................... 193

Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Regulations for the use of the collective Naktuinbouw Quality Brand version 3 01-12-2020 Page 2 of 195

Regulations on the use and control of the Collective

Naktuinbouw Quality Brand ‘Elite’ (Article 2.37 of the Benelux Convention on Intellectual Property)

2.1 Article 1

Definitions:

a. Holder of the registered collective trademark is Stichting Nederlandse Algemene

Kwaliteitsdienst Tuinbouw [Netherlands Inspection Service for Horticulture]

in Roelofarendsveen, hereinafter to be referred to as the “Holder”;

b. User of the Collective Mark is each Registered Party which has been explicitly

authorised by the Holder rand to which the assurance of the Collective Mark

applies;

c. Registered Party is the natural person who, or legal person which, is registered with

Naktuinbouw as referred to in article 42 of the Dutch Seeds and Planting

Materials Act 2005 [Zaaizaad- en Plantgoedwet 2005] and who/which, by

virtue of their profession, performs at least one of the following activities

with regard to propagating material: propagating, producing, processing –

other than for use in the Registered Party’s own business – and/or

marketing;

d. Collective Mark is the word/pictorial mark and/or logo shown below, all the rights, including

copyrights, in which are vested in the Holder pursuant to the provisions of

article 2.34 ff. of the Benelux Convention on Intellectual Property. The

Collective Mark pertains to agricultural, horticultural, and forestry products,

insofar as they are not included in other categories; seeds for sowing, live

plants and flowers, plant parts, fresh fruits and vegetables, vegetable seeds;

propagating material and nursery stock tissue culture material, as well as

laboratory services, in particular for the above-mentioned goods / products;

inspection, checking and certification services in the field of the quality

improvement and control of the above-mentioned goods; services in the field

of testing standards, certification criteria and assessment guidelines; testing

quality improvement, control and assurance systems on the basis of the

applicable requirements; testing services, products and processes on the

basis of standards, certification criteria and assessment guidelines;

supervising the quality of services, products and processes of the third

parties; providing certificates to such third parties; drawing up and issuing

objective quality statements.

Image of logo


e. Material is the propagating material produced by the Registered Party, such as

graftwood, budwood, dormant buds, rootstocks, cuttings, and certified

planting material from which certified plants have been obtained, the current

condition of which complies with the requirements based on the Dutch Seeds

and Plating Materials Act, the Dutch Plant Diseases Act [Plantenziektenwet],

and any rules and regulations based thereon, and based on the Naktuinbouw

Certification Regulations [Certificeringsreglementen Naktuinbouw] for the

crop concerned as indicated below in article 2, to, on, in, or for which

material, and, if so desired, its packaging, advertising, price lists, brochures,

invoices and/or on stationery, the Registered Party can apply the Collective

Mark as a quality assurance, however any pursuant to the explicit conditions

of article 8 of these Regulations;

f. Removal of the Collective Mark is the rendering completely illegible and/or removing

of the Collective Mark from packaging materials for the Material and/or from

the Material itself, as well as from advertising, price lists, brochures, invoices

and/or from stationery, insofar as the material, to, on or for which the

Collective Mark has been applied does not, or no longer, comply with the

applicable quality requirements.

2.2 Article 2

The Holder allows the non-exclusive use of its Collective Mark to all Registered Parties registered

with Naktuinbouw, which trade in Materials that at least meets the requirements defined in:

a. The Dutch Seeds and Planting Materials Act 2005 and the Dutch Plant Diseases Act and any rules

and regulations based thereon;

b. The Naktuinbouw Inspection Regulations;

c. The Naktuinbouw Certification Regulations of the crops concerned;

d. The regulations of the Naktuinbouw Management Team that are based on points a., b. and c.

above;

e. The Conditions on the use of the Naktuinbouw Elite logo,

and;

f. Any other new and/or modified legislation, rules and/or regulations that have come into effect

after the date on which these Regulations were drawn up.

2.3 Article 3

In order to qualify for the non-exclusive use of the Collective Mark, each Registrated Party shall in

advance, by a written agreement concluded with Naktuinbouw, undertake to comply unconditionally

with anything which has been, or will be, stipulated in, or pursuant to, the Articles of Association and

Regulations of Naktuinbouw, i.e. the Holder.

2.4 Article 4

For the non-exclusive use of the Collective Mark being granted, each User shall pay an annual

contribution in advance each year, which will be determined by the Naktuinbouw Board. This

amount shall also be determined by how the costs required for maintaining the Naktuinbouw


organisation, which is responsible for the management and control of the use of the Collective Mark,

are spread among Users.

2.5 Article 5

1. The Holder supervises compliance with these Regulations itself or can, through a license,

delegate the management of such supervision to persons and/or institutions appointed by the

Holder. Such supervision shall comprise:

a. administrative check;

b. Sampling and research;

c. Visual checks of Material and Material packaging;

d. Other inspections and checking methods as deemed necessary by the Holder to maintain the

quality standards.

2. Users are not authorised to transfer the right to use the Collective Mark to any third party or to

grant a licence to any third party for its use.

2.6 Article 6

1. User shall allow supervisors, inspectors and auditors appointed by Naktuinbouw, who shall

identify themselves for such purpose beforehand, to enter – during regular office hours –

industrial spaces and/or other storage facilities, and/or cultivation areas where the User

performs, or has third parties perform, activities that are related to the trading of

propagating materials, the quality standards of which are assured by the Collective Mark.

2. All Users of the Collective Mark shall immediately notify the Naktuinbouw Board of any

infringement on the Collective mark that comes to their knowledge.

2.7 Article 7

A report shall be drawn up of any check or inspection referred to in article 6 within five days of such

check or inspection, and a copy of such report will subsequently be sent to the User if the quality

standards have not been met or not been fully met.

2.8 Article 8

Users are entitled to apply the Collective Mark to their materials and/or product packaging materials

and place it in advertising, price lists, brochures, invoices and/or on stationery and suchlike as quality

assurance, provided that such right has not been withdrawn from them for another reason. The

above use of the Collective Mark by Users shall only be allowed in relation to the certified product in

question. There shall be a clear relationship between the Collective Mark and the relevant product

that has been produced under the certification at all times, as to avoid third parties being misled.

This means that using the Collective Mark in general statements that have no direct bearing to the

certified product in question, for example on cars, stationery, in e-mail signatures, etc., is not

allowed.

If the requirements for the use of the Collective Mark pursuant to these Regulations are not met, or

are no longer met, the User or former User shall remove the Collective Mark in accordance with

article 1.f.

2.9 Article 9

1. Any infringement by a User of their/its obligations arising from these Regulations, including

the provisions of article 2, and/or of the agreement concluded with the Holder, shall

constitute a violation. The Holder can impose the following sanctions on the User who or


which uses the Collective Mark inappropriately for purposes other than those provided for by

these Regulations and the agreement:

a. a warning, accompanied by an instruction as to which internal measures will have to be

taken by the User in order to be able to comply with the requirements of these

Regulations in the future;

b. a one-off, immediately payable penalty of a maximum amount of € 225.000,

c. a temporary prohibition against using the Collective Mark for a period of a maximum of

two years and/or a prohibition against using the Collective Mark for one or more type(s)

of materials or products;

d. complete disqualification from using the Collective Mark;

e. a prohibition pursuant to c. above, and/or disqualification pursuant to c. above may be

accompanied by coercive measures being imposed by the Holder, such as a penalty in the

amount of a fine as referred to in b. above, which the User in question shall forfeit for

any and each violation of the prohibition imposed;

f. publication of a sanction or sanctions imposed on the User, stating the reasons of such

prohibition, in one or more newspapers and/or trade journals.

2. When imposing a sanction as described in the previous paragraph under b. to f., the Holder

shall always state the legal remedies that are available.

3. The User of the Collective Mark shall no longer be bound by these Regulations, if and as soon

as the Collective Mark is cancelled, declared void or stricken from the register. In derogation

of the above, the User shall continue to be bound to the measures and sanctions as referred

to in paragraph 1 of the article with regard to violations that took place during the time when

the Collective Mark was still registered.

2.10 Article 10

If the User uses the Collective Mark without all due care, thereby causing loss of the Holder or other

Users, the Holder shall be entitled to take legal action, either individually or together with other

Users, to claim such loss from the User first mentioned. The costs of such claims shall be fully at the

expense of such User.

2.11 Article 11

The Holder has the exclusive right to take legal action to protect the Collective Mark. When

exercising such right, the Holder may also take into account the general interest of any other Users of

the Collective Mark, including any loss incurred by them in its claim for damages. However, if desired,

any User of the Collective Mark can institute a claim together with the Holder, and/or participate in

such proceedings in any other way.

2.12 Article 12

1. Without prejudice of the provisions of the Benelux Convention on Intellectual Property and

the provisions of article 9.3 of these Regulations, the right to use the Collective Mark shall

end:

a. by withdrawal of the right of use pursuant to the stipulations of the opening sentences

and point d. of article 9;

b. by termination of the Registered Party’s registration with Naktuinbouw and


c. if a User, also after a reminder, remains in default to pay Naktuinbouw the contribution

referred to in article 4 within 30 days.

2. If a User’s right ends pursuant to the stipulations of paragraph 1, such User shall not be

entitled to compensation of any loss incurred due to such User no longer being allowed to

use the materials referred to in article 1.e. to which the Collective Mark is applied.

2.13 Article 13

1. Any additions to, and/or modifications of, these Regulations shall come into effect

immediately following their announcement by registration with the Benelux Office for

Intellectual Property. The Holder shall ensure that such additions and/or modifications are

registered with this Office.

2. Resolutions to modify these Regulations can be adopted at a meeting of the Naktuinbouw

Board, with due observance of the requirements on adopting legally valid resolutions as

provided by the foundation’s Articles of Association.

2.14 Article 14

The Holder shall provide each User of its Collective Mark as well as any interested third parties a free

copy of these Regulations.

Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Agreement version 8 01-01-2020 Page 7 of 195

Participation agreement for Naktuinbouw Elite collective

trademark

The undersigned:

A. (name company) in (place), registered with Naktuinbouw under number ………….., hereafter

to be called the ‘Participant’;

and

B. Stichting Nederlandse Algemene Kwaliteitsdienst Tuinbouw (Naktuinbouw), PO Box 40,

Sotaweg 22, 2370 AA Roelofarendsveen, The Netherlands, legally represented for these

purposes by Mr R.A.Th. Bleijswijk, Head of Inspections Department;

Hereby agreed as follows:

1. Naktuinbouw shall, financially compensated by the participant, observe provisions and

conditions mentioned in the Regulations on the use of the collective trademark Naktuinbouw

Elite and the Certification Regulations, added as an Appendix to this Agreement. The

Participant accepts these provisions and conditions unabridged.

2. The under 1. mentioned financial compensation is charged on the basis of tariffs fixed by the

Board of Naktuinbouw.

3. This agreement shall endure for a period of 3 years, starting on (date) and expiring on (date

3 years later). At the moment of expiry, this agreement is automatically prolonged for a

period of one year, unless, at least 3 months before the expiry date, one party has informed

the other party that the agreement is not going to be prolonged and therefore will be ended.

4. In case of changing the Regulations on the use of the collective trademark (Naktuinbouw

Elite or Certification Regulations), the participant has the right to reject these changes, by

letter, within two months after notice, fully and unconditionally. Naktuinbouw and/or the

participant then have the right to end this agreement.

5. The General Terms and Conditions of Naktuinbouw are applicable to this agreement, as well

as the Regulations on the use of collective trademark Naktuinbouw Elite. In the event of a

dispute regarding the interpretation of this Agreement, the English text will be decisive.

6. If the participant fails to comply with the conditions of this Agreement, Naktuinbouw may, at

its discretion, after a warning in the form of a registered letter or bailiff’s writ, declare this

Agreement dissolved, without any judicial intervention being required, in which case

Naktuinbouw reserves the right to claim the loss actually incurred.

Drawn up in duplicate originals in Roelofarendsveen, The Netherlands on (date)

Naktuinbouw Participant:

R.A.Th. Bleijswijk, (name company)

Head of Inspections Department (name person)

Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Certification Regulations Naktuinbouw Elite Ornamental Crops version 9 01-01-2021 Page 8 of 195

Certification regulations Naktuinbouw Elite Ornamental Crops Determined by the Naktuinbouw Board dated 18 December 2020

4.1 Article 1 – Definitions

These Regulations follow the definitions from the "Keuringsreglement Naktuinbouw" (Naktuinbouw

Inspection Regulations), unless expressly stated otherwise. The meaning of the definitions is

described in chapter 32 Definitions / references.

4.2 Article 2 - Applicability

1. These Regulations apply to propagation material, commercially produced and presented to

Naktuinbouw for certification, of species and varieties of ornamental crops as mentioned in

Appendix I to this Regulations.

2. Any propagation material and plant material presented by a supplier for certification which does

not satisfy the requirements as layed down in the EU directive 98/56, will not be eligible for

certification.

3. This Regulation does not apply to propagation material that is not inspected by Naktuinbouw,

except when it is produced in a propagation- and certification scheme that is declared

equivalent by Naktuinbouw.

When the requirements mentioned above are fulfilled, on request of the participant

propagation material can be brought into the propagation and certification schemes inspected

by Naktuinbouw and certification inspections can be carried out. The participant has to hand

over to Naktuinbouw all the relevant documents, including the document or certificate, that at

as a minimum contains the following information: country of origin, class of certification and

name of the official organization that has formally carried out the certification.

4.3 Article 3 - Notification

1. Crops must be presented to Naktuinbouw for certification, at the commencement of

cultivation, by means of a form provided by Naktuinbouw, or in any other manner accepted

by Naktuinbouw.


4.4 Article 4 - Requirements imposed on the company management and obligations of

the supplier

1. The participant must have implemented a quality management system compliant to

Appendix VI ‘Naktuinbouw Module Quality Management System Requirements / Sampling

Requirements’ and the participant must:

a. provide a map of the company to Naktuinbouw. The map must show in which parts

of the company the Naktuinbouw Elite propagation material is produced;

b. implement procedures and work instructions for activities that can have impact on

the quality and health of the propagating material;

- there must be an overall plan concerning the production and processing of

the propagation material;

- the production must be carried out under controlled conditions;

- ensure complete traceability of propagation material. All the lots of mother

plants, seed plants and/or propagating material should be clearly

identified;

- ensure that the propagation material has been stored, handled,

transported and delivered under proper conditions (including final

inspection and delivery documents).

2. A participant can become eligible for certification inspection when an audit of the quality

management system, carried out by Naktuinbouw, has shown that requirements mentioned

under paragraph 1. are fulfilled.

3. Sampling as described in Appendix IIA must be carried out compliant to Appendix VI

‘Naktuinbouw Module Quality Management System Requirements / Sampling Requirements’

and testing of these samples can be carried out by the participants’ own laboratory or external

laboratory.

If testing is carried out by the laboratory of Naktuinbouw or by an ASLN authorized

laboratory, Naktuinbouw control sampling as stated in Appendix IIB, will be reduced

according to Appendix IIC.

4. a. The participant must in conformity with regulations in the country of production, report to the official authorities all relevant quarantine organisms found in the crops or in propagation material.

b. The participant reports immediately to Naktuinbouw when a quarantine organism

relevant in Regulation (EU) 2016/2031 and or mentioned under 4a, is found.

5. The participant has the obligation to report immediately to Naktuinbouw the presence of

pathogens that prevent the classification of material into any certification class.

6. The participant has to declare to Naktuinbouw that he/she, by producing or supplying

propagation material, operates in full compliance with regulations concerning breeders

rights and (intellectual) ownership/properties. This written and signed declaration shall be

included in the company’s file kept in Naktuinbouw’s archives.


4.5 Article 5 - Cultivation Requirements

1. All necessary care must be demonstrably taken of the crop, in particular in regard to crop

protection and weed prevention. Sufficient monitoring for possible diseases and crop

protection has to be carried out. Naktuinbouw can prescribe further specific requirements.

2. The company must observe rules of good hygiene practice. Particular attention must be paid

to:

a. the presence and use of disinfectants and disinfectant footh baths (footh mats and/or other sufficient disinfection methods);

b. the presence and use of sticky traps; c. regular disinfecting of tools; d. the wearing of clean clothing by all employees involved; e. a smoking ban in all compartments or rooms where mother plants, seed plants or

propagation material is produced, rooted, or handled; f. a disease-free growing medium; g. disinfection of materials before use; h. regular disinfecting of all compartments or rooms where motherplants, seed plants

or propagation material is produced, rooted, or handled.

3. Water must be demonstrably free from relevant pathogens, see chapter 26 Instruction

sampling plant material and water and chapter 28 Instruction disinfecting water. Surface

water or recycled water can only be used after effective disinfection has taken place (this at

the discretion of Naktuinbouw).

4. Mother plants, seed plants, cuttings, seedlings and grown plants must be cared for and

grown in such a way and under such hygienic conditions, that infection by pathogens is

actively prevented.

4.6 Article 6 - The Certification Inspection

1. The certification inspection is effected by means of:

a. auditing of production and handling of certified propagation material;

b. checking the origin of the material used for propagation;

c. sampling and testing of mother plants, seed plants and/or propagation material;

d. random control of mother plants, seed plants and propagation material;

e. control of propagation material after delivery.

2. Naktuinbouw will plan and carry out inspections, controls and tests as deemed necessary.

Naktuinbouw determines the frequency, the effective data and the way inspections, controls

and tests are executed.

3. If it is found that batches of mother plants, seed plants and/or propagation material during

cultivation show symptoms of the diseases mentioned in Appendix III to these Regulations,

the participant has to take appropriate measures as referred to in the said Appendix.

4. By conducting certification inspections gaps will be left out of consideration, provided that it

is satisfactorily proven that such gaps were caused by removal or dying off rather than as a

result of disease.


5. If, at any point, it is found that propagation material fails to satisfy the requirements of these

Regulations or specifications based thereon, no certification can take place. If the participant

finds that propagation material is not satisfying these Regulations he will report these finding

to Naktuinbouw immediately. If measures have subsequently been taken to the satisfaction

of Naktuinbouw, application may be made for a re-assessment, so that the propagation

material may again be certified if at the time of such a re-assessment it satisfies all the

requirements.

4.7 Article 7 - Classification

1. A crop and/or propagation material originating therefrom will be certified if official

inspection(s) has (have) shown it to be true-to-type, true to variety and sufficiently healthy,

as set out for the class and/or varieties concerned in Appendixes I, II and III to these

Regulations, and if in addition it meets all other quality requirements for certified material as

mentioned in the Appendixes.

2. On certification, the propagation material is classified Class SEE, Class EE, or Class E.

3. The classification as referred to in article 7.2 will on delivery be combined with the designation:

« Naktuinbouw Elite ».

4.8 Article 8 - Candidate Plants

1. Candidate plants are plants selected by the participant for introduction into the propagation

system used for production of certified material that have to undergo all the prescribed

tests.

2. Candidate plants must be grown in an aphid-proof greenhouse or compartment provided

with a double-door entrance, in which no plants and/or propagation material of any

certification class are kept, not in contact with the floor, in separate pots or containers,

under strict hygiene conditions. The candidate plants must be tested in a way approved by

Naktuinbouw for all pathogens, as set out in Appendix IIA to these Regulations, and must

further be inspected in particular for varietal purity.

3. Candidate plants must be free from the diseases mentioned in Appendix III to these

Regulations.

4. Candidate plants or their offspring that have been found free from pathogens as mentioned

in Appendix II will be considered by Naktuinbouw as pathogen free material and may be

introduced in the SEE1-class of the propagation of the participant. To this end, the participant

shall submit the findings of tests conducted on candidate plants.

4.9 Article 9 - Classification under Class SEE

1. (Pre-basic) mother plants and/or in-vitro propagated plants will be classified by Naktuinbouw

under Class SEE if:

a. they have been propagated directly from material as referred to in paragraph 4 of

Article 8, or from Class SEE propagation material, or from Class EE certified material,

or obtained from other material which is regarded by Naktuinbouw as being

equivalent;

b. they are grown free of contact with the ground in an aphid-proof greenhouse or

compartment provided with a double-door entrance in which no other plants are


grown than SEE plants or in a tissue culture laboratory. Pre-basic mother plants must

be grown in separate pots or containers;

c. during the growing season, prior to the production of cuttings, all mother plants have

been tested for pathogens as set out in Appendix IlA and IIB, and have been found to

be free of infections;

d. an identity control as described in Appendix I is carried out; and it is proven the

plants are true to type;

e. the results of certification inspections carried out by Naktuinbouw show all

requirements have been fulfilled. Naktuinbouw executes during these inspections

the tests prescribed in Appendix IIB. If testing of the samples ‘control participants’ is

carried out by Naktuinbouw laboratory or by an ASLN authorized laboratory,

Naktuinbouw control sampling as stated in Appendix IIB, will be reduced according

to Appendix IIC.

2. Propagation material will be classified under Class SEE:

a. if it originates directly from Class SEE mother plants, or from tissue culture material

that has the SEE status;

b. if rooting or cultivation takes place in tissue culture and /or in an aphid-proof

compartment free of contact with the floor, under conditions as laid down for the

growing of SEE mother plants. Cuttings derived directly from SEE mother plants may

be rooted in the compartment where SEE mother plants are present.

3. To test trueness to variety and health of SEE-stock that is maintained in tissue culture, on a

periodic basis a representative sample has to be grown to flowering. The prescribed identity

control and their frequencies (Appendix I) and health controls (Appendix II) are carried out on

this material.

4. Classification under class SEE may only take place if the tolerance in respect of diseases and

pests, described for this class in Appendix III to this regulation have not been exceeded. On

the establishing of the presence of a disease or pest the infected plant must be removed

immediately, including any plants which are in contact with the infected plant.

5. a. At the entrance of a SEE greenhouse or compartment a trough drenched mat with

sufficient disinfectants and/or other sufficient disinfection methods have to be in

place.

b. Only employees who have to carry out designated tasks, employees on duty from

official organisations who have to execute inspections or employees of Naktuinbouw

who carry out audits or controls are permitted into the greenhouse or compartment

or tissue culture laboratory for execution of their tasks.

All persons who enter the greenhouse or compartment or tissue culture laboratory

have to carry out all measures to prevent infection, such as disinfecting of hands and

shoes, and wearing of prescribed clothing, conscientiously. Hygiene and access

regulations must be clearly visible at the entry of these facilities.

4.10 Article 10 - Classification under Class EE

1. Basic mother plants and/or seed plants or in-vitro propagated plants will be classified by

Naktuinbouw under Class EE if:

a. the crop consists of plants grown directly or indirectly (in no more than one


propagation step) from propagation material classified under Class SEE or from Class

E certified material. If more than one propagation step is allowed it is mentioned in

Appendix II;

b. they are grown free of contact with the floor in an aphid-proof compartment

provided with a double-door entrance in which no other than Class EE plants are

grown or in a tissue culture laboratory;

c. during the growing season, prior to production of cuttings the mother plants have

been tested for pathogens as indicated in Appendix IIA and IIB; the findings shall be

submitted to Naktuinbouw;

d. an identity control as described in Appendix I is carried out and trueness to variety is

proven;






to Appendix IIC.

2. Propagation material (seeds, seedlings, cuttings, plants) will be classified under Class EE:

a. if it originates directly from class EE mother plants, seed plants or from tissue culture

material, that has the EE status;

b. if rooting or cultivation takes place free of contact with the ground, in an aphid-proof

compartment under conditions as laid down for the growing of EE mother plants.

Cuttings derived directly from EE mother plants may be rooted in the compartment

where EE mother plants are present.

3. Classification under class EE may only take place if the tolerance in respect of diseases and

pests as specified for this class in Appendix III to these Regulations have not been exceeded.

On the establishing of the presence of a disease or pest the affected plant must be removed

immediately, including any plants which are in contact with the affected plant.

4. a. At the entrance of an EE greenhouse or compartment a trough drenched mat with

sufficient disinfectants and/or other sufficient disinfection methods have to be in

place.

b. Only employees who have to carry out designated tasks, employees on duty from

official organizations who have to execute inspections or employees of Naktuinbouw

who carry out audits or controls are permitted into the greenhouse or compartment

or tissue culture laboratory for execution of their tasks.

All persons who enter the greenhouse or compartment or tissue culture laboratory

have to carry out all measures to prevent infection, such as disinfecting of hands and

shoes, and wearing of prescribed clothing, conscientiously. Hygiene and access

regulations must be clearly visible at the entry of these facilities.

4.11 Article 11 - Classification under Class E

1. Certified mother plants and/or seeds plants will be classified by the Naktuinbouw under

Class E if:

a. the crop consists of plants grown directly from propagation material classified under


Class EE (or SEE). If more propagation steps are allowed its mentioned in Appendix II;

b. they are preferably grown free of contact with the ground in a compartment in which

no plants other than certified mother plants or material that is from a disease free

heritage are grown or in a tissue culture laboratory. Cuttings directly originating from

these basic mother plants may be rooted in this compartment;

c. during the growing season prior to production of cuttings the mother plants have

been tested for pathogens as indicated in Appendix IIA and IIB; the findings shall be

submitted to Naktuinbouw;

d. an identity control as described in Appendix I is carried out and trueness to variety is

proven;






to Appendix IIC.

2. Propagation material (seeds, seedlings, cuttings, plants) will be classified under Class E:

a. if it originates directly from at least Class EE mother plants; seed plants or from tissue

culture in vitro material that has the E status;

b. if rooting or cultivation takes place preferably free of contact with the ground,

preferably in an aphid-proof compartment under conditions as laid down for the

growing of E mother plants. Cuttings derived directly from E mother plants may be

rooted in the compartment where E mother plants are present.

3. Classification under Class E may only take place if the tolerance in respect of diseases and

pests as specified for this Class in Appendix III to this regulation has not been exceeded. On

the establishing of the presence of a disease or pest the affected plant must be removed

immediately, including any plants which are in contact with the affected plant.

4. a. At the entrance of an E compartment a through drenched mat with sufficient

disinfectants and/or other sufficient disinfection methods have to be in place.

b. All persons who enter the greenhouse, compartment or tissue culture laboratory

have to carry out all measures to prevent infection. Hygiene and access regulations

must be clearly visible at the entry of these facilities.

4.12 Article 12 - Quality

Marketed propagating material/plants must be sufficiently developed, well rooted and substantially

free from damage. It has to fulfill common trade requirements.

4.13 Article 13 - Identification and Documents

1. Propagation material classified under Class SEE or Class EE, must during release for

circulation and sale, be accompanied by a certificate conforming to the specimen shown in

Appendix V to these Regulations, while in addition the specified contents must be clearly

shown on the packaging.


2. A certificate will be issued per batch. Two or more batches may be described on one

certificate, if the same period of validity applies to the batches concerned, and if these

batches are of the same class of certification.

3. The certificate will only be valid if initialed by the authorized officer of the company. If

delivery does not take place within ten days of the date of issue of the certificate, the

certificate will cease to be valid.

4. On each packaging unit of SEE and EE material it must be clearly indicated, by means of a

label, what material is contained in the packaging and of which class it is.

5. Conditions may additionally be attached to a certificate. These conditions will be stated on

the certificate.

6 a. Propagation material classified under Class E must, during release for circulation and

sale, be accompanied by a delivery sheet (supplier’s document) written out by the

participant. On this delivery sheet each batch of propagation material that is Class E

certified must be indicated.

If the participant wishes to use a Naktuinbouw Elite indication on his/her delivery

sheets and/or labels he/she needs, prior to any use, a written consent from

Naktuinbouw.

b. On his/her request Naktuinbouw can supply the participant with a certificate for his E

Class propagation material. In that case paragraph 2, 3, and 5 of this article become

valid.

4.14 Article 14 - Withdrawal of Certificates

1. Naktuinbouw decides upon applications for the issue of a certificate.

Naktuinbouw has the right to withdraw an issued certificate which has been issued earlier, if

it is established that the material in question or the supplier in question no longer satisfies

the requirements in force, and furthermore, the issuing of certificates shall be suspended in

those cases where the director of Naktuinbouw has established that there has been an

infringement.

2. The supplier may lodge an objection with Naktuinbouw within five days against the decision

of Naktuinbouw not to issue a certificate, including the failure on the part of Naktuinbouw to

make such decision in good time.

3. In the event of the supplier failing to comply with and being in default of fulfilling his financial

obligations towards Naktuinbouw, including those arising otherwise than under certification,

Naktuinbouw may suspend its certification activities until the date of payment in full by the

supplier.

4.15 Article 15 - Final Provisions

1. These Regulations may be referred to as the « Certification Regulations Naktuinbouw Elite

Ornamental Crops »

2. In respect of any cases not provided for in these Regulations, the Board of Naktuinbouw shall

decide.


3. These Regulations will come into force on 1 January 2021.

The by the board of Naktuinbouw approved English version of these Regulations prevails.

Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Appendix I Specific requirements for identity, trueness to variety and trueness to type.., version 14 01-01-2020 Page 17 of 195

Appendix I Specific requirements for identity, trueness to variety

and trueness to type for propagation material of ornamental

crops to be accepted for certification

Propagation material of species and varieties of ornamental crops is eligible for certification if the

variety:

- is listed in the Dutch Register of Varieties ("Nederlandse Rassenregister") or the

European Register of Varieties ("Europees Rassenregister") and/or

- is included in the registration and documentation system of the Floricode (Roelofarendsveen,

The Netherlands) and/or

- is included in the registration and documentation system of the KAVB (Hillegom, The

Netherlands) and/or

- is included in the registration and documentation system of Naktuinbouw and /or

- is described and identified in such a manner that in the opinion of Naktuinbouw they satisfy

the variety description requirements.

A variety description has to be presented as part of the first application for certification. The

participant should periodically submit a list of certifiable varieties to Naktuinbouw.

5.1 Identity

5.1.1 General

For varieties that are sensitive to sporting (mutation), also called unstable varieties, the following

regulation is prescribed.

To determine whether propagation material is true to variety and true to type the participant has to

check before certification each plant or clone (100%) for proper flower bearing properties or other

identity features, based on the variety description.

Deviant plants must be removed.

Naktuinbouw can prescribe that varieties are placed on a list of varieties that are sensitive to

sporting (mutation). For all varieties placed on this list this specific requirement is obligatory.

Further requirements for the inspection for identity of propagation material of the listed species and

varieties in Class SEE, Class EE and Class E.

5.1.2 Class SEE



identity features, based on the variety description. In case propagation material is only used for

internal use, certification of SEE material is permitted under strict conditions. Identity control needs

to be finished before certification of next Class.

When maintaining SEE-stock in tissue culture, a yearly identity control is prescribed.


Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Appendix I Specific requirements for identity, trueness to variety and trueness to type.., version 14 01-01-2020 Page 18 of 195

5.1.3 Class EE



identity features, based on the variety description.


5.1.4 Class E

No control prescribed.

Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Appendix II Requirements for the testing for pathogens of propagation material of ornamental crops, version 16 01-01-2021 Page 19 of 195

Appendix II Requirements for the testing for pathogens of

propagation material of ornamental crops

Appendix II.A Control by participant For the purposes of certification the participant must be able to produce a written record of the

results of the tests for the pathogens mentioned in this Appendix II. Crops (in the case of candidate

plants) originating from outside the EU, must be documented as proven free of relevant quarantine

organisms.

Crops originating from in vitro propagation (tissue culture) or grown in vivo must be grown in a

growing medium in vivo-conditions for at least 4 weeks* before sampling and testing takes place. *[Rosa L.; this will take at least one growing season]

When more than one test is prescribed, the waiting period between tests is at least 4 weeks*. *[ Rosa L.; this will take at least 8 weeks.]

Tests have to be carried out in a way prescribed by Naktuinbouw or accepted by Naktuinbouw.

Companies must maintain a clear and understandable procedure and records for their test program.

If testing of the samples ‘control participant’ is carried out by Naktuinbouw laboratory or by an ASLN

laboratory, Naktuinbouw control sampling as stated in Appendix IIB, will be reduced according to

Appendix IIC.

Naktuinbouw can, in addition to these Regulations, prescribe that testing for specific (other)

pathogens is carried out.

7.1 Candidate Plants

All plants have to be tested at least twice* for viruses (C1 and C2) of all standard pathogens significant

for the family or genus (standard pathogens). *[Rosa L.; testing will have to be conducted at least once in Spring]

7.1.1 C1 (indicator plants)

For at least one of the two virus tests, indicator plants must be used (except when the candidate

plant originates from pathogen free breeding conditions, accepted by Naktuinbouw.* *[Compartment with plants grown used for breeding purposes. Plants have to be tested for relevant pathogens. This to be determined by

Naktuinbouw]

7.1.2 C2 (pathogens)

Furthermore, all plants must be tested once for all other pathogens that are significant for the family

or genus (standard pathogens/additional pathogens).

All candidate plants are to be found free of pathogens and may not induce symptoms on indicator

plants, before it is possible to move on to the SEE phase. The participant must submit a testing report

along with the first request for certification for class SEE. Candidate plants also must be free of

symptoms caused by pathogens which are not mentioned. Participants must be able to produce

written records of the results of the tests to Naktuinbouw.

When candidate plants originate from countries outside the EU, tests for other pathogens can be

prescribed.


7.2 Pre-basic mother plants SEE (nuclear stock)

7.2.1 SEE1 (plants and clones produced directly from Candidate plants)

7.2.1.1 In vivo propagation

Before production of propagating material takes place, all individual plants have to be tested once

for pathogens that are significant for the family or genus (standard pathogens).

Furthermore, all plants must be tested once for all other pathogens that are relevant for the family

or genus (additional pathogens) during the growing season.

Any plant showing symptoms that might indicate viral diseases or bacterial diseases must be tested

immediately. Each plant that is found to be infected must be removed by the participant.

7.2.1.2 In vitro propagation (tissue culture)

Before production of propagating material takes place, all clones have to be tested once for

pathogens that are significant for the family or genus (standard pathogens).

Furthermore, all clones must be tested once for all other pathogens that are relevant for the family

or genus (additional pathogens) during the growing season.

When maintaining and producing a variety in a participant’s tissue culture laboratory, no testing is

prescribed thereafter (regardless of the duration) except in specific situations (these situations to be

determined by Naktuinbouw).

7.2.2 SEE2 (plants or clones produced directly from SEE1 or SEE2)


Before production of propagating material takes place, the percentage of plants mentioned in the

table have to be tested once for pathogens that are significant for the family or genus (standard

pathogens).

Furthermore, the percentage of plants mentioned in the table must be tested once for all other

pathogens that are relevant for the family or genus (additional pathogens) during the growing

season.



7.2.2.2 In vitro propagation (tissue culture) derived from vivo propagation

Before production of propagating material takes place, all clones have to be tested once for

pathogens that are significant for the family or genus (standard pathogens).

Furthermore, the percentage of clones mentioned in the table must be tested once for all other


season.

7.2.3 SEE (plants or clones derived from certified vivo material)


If certified vivo material is upgraded to class SEE¹ or EE, the percentage of plants mentioned in the

table for its class have to be tested once for pathogens that are significant for the family or genus

(standard pathogens). Furthermore, the percentage of plants mentioned in the table must be

tested once for all other pathogens that are relevant for the upgraded class and family or genus


(additional pathogens) during the growing season. The tests must be carried out before the

production of propagating material takes place.

Upgrading of vivo material may only be done in case of problems with crops and after approval of

Naktuinbouw.

7.2.3.2 In vitro propagation (tissue culture)

If certified vivo material is upgraded to in vitro, the percentage of plants mentioned in the table for

class SEE¹ have to be tested once for pathogens that are significant for the family or genus

(standard pathogens). Furthermore, the percentage of plants mentioned in the table class SEE¹

must be tested once for all other pathogens that are relevant for the upgraded class and family or

genus (additional pathogens) during the growing season. The tests must be carried out before the

production of propagating material takes place.

Upgrading of vivo material may only be done in case of problems with crops and after approval of

Naktuinbouw.

Once a variety, certified as Class SEE, has been included in another participant’s tissue culture

laboratory for purposes limited to production, no testing is prescribed except in specific situations

(to be determined by Naktuinbouw).

No testing is prescribed thereafter (regardless of the duration) except in specific situations (to be

determined by Naktuinbouw).

Crops may not be infected by Tobamo-viruses (ToMV-D-O3, TMV-WU1), Ralstonia solanacearum,

Xanthomonas hortorum pv. pelargonii, Xanthomonas axonopodis pv. dieffenbachiae,

Agrobacterium tumefaciens or Fusarium foetens. When infection takes place, retesting of all

possible infected plants is prescribed.

7.3 Basic mother plants EE (Propagation Stock)

Before production of propagating material takes place, the percentage of plants or clones (number of

samples), referred to in this Appendix, per compartment or company location have to be tested at

least once for the pathogens that are significant for the family or genus (standard pathogens).

Furthermore, the percentage of plants mentioned in the table must be tested once for all other


season.







7.4 Certified mother plants E (Certified Stock)

Before production of propagating material takes place, the percentage of plants or clones (number of

samples), referred to in this Appendix, per compartment or company location have to be tested at

least once for the pathogens that are significant for the family or genus (standard pathogens).

Furthermore, the percentage of plants mentioned in the table must be test at once for all other



season.







Handbook Naktuinbouw Elite Ornamental Crops Back to table of contents Appendix II.A Control participants protocols, version 19 01-01-2021 Page 23 of 195

Appendix II.A Control participants protocols

Family: Acanthaceae

Genus: see appendix II.D

Standard pathogens C1 Indicator

plants C2 SEE1 SEE2 EE E

Test method

Remarks

Percentage plants 100% 100% 100% 10% 10% 1%

number of samples minimum/maximum

100/

1.000 100/

1.000

AMV Cq and No

X X ELISA See chapter 26 and chapter 27

CMV X X X X X ELISA or PCR

See chapter 26 and chapter 27

Potyvirus (PVY, PVA, LMV)

X X X X X ELISA See chapter 26 and chapter 27

ToMV-D-03 X X X X X ELISA See chapter 26 and chapter 27

TMV-WU1 X X X X X ELISA See chapter 26 and chapter 27

TSWV X ELISA See chapter 26 and chapter 27

INSV X ELISA See chapter 26 and chapter 27

ArMV X ELISA See chapter 26 and chapter 27

TRV X ELISA or PCR


BBWV1 X ELISA See chapter 26 and chapter 27


AltMV X ELISA See chapter 26 and chapter 27

Family: Acoraceae

Genus: Acorus



Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No









TRV X ELISA or PCR



Family: Agapanthaceae

Genus: Agapanthus



Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No




EMDV X ELISA See chapter 26 and chapter 27



ToMV-D-03 X X ELISA See chapter 26 and chapter 27

TMV-WU1 X X ELISA See chapter 26 and chapter 27




TRV X PCR See chapter 26 and chapter 27

NVX X X X X X ELISA/ PCR


Family: Aizoaceae




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR



Family: Alstroemeriaceae

Genus: Alstroemeria



Test method

Remarks



* * 100/

1.000 100/

1.000

* Only tissue culture propagated material permitted

CMV

Cq and No and

Nb X X

ELISA or PCR


AIMV X X X X X ELISA See chapter 26 and chapter 27

ALCV (LSV) X X ELISA See chapter 26 and chapter 27



IYSV X ELISA See chapter 26 and chapter 27


Family: Amaranthaceae




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR



Additional pathogens

Pospiviroids (IrVd-1)

X

PCR Pospi1/Vid primers or Taqman RT-PCR

Celosia Iresine See chapter 26 and chapter 27

TSV Cq and

No X ELISA

Gomphrena



Family: Apiaceae

Genus: Astrantia



Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No












ToRSV X ELISA See chapter 26 and chapter 27


Family: Apocynaceae




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR



Phytoplasma

X PCR Catharanthus See chapter 26 and chapter 27

Pospiviroids

X

PCR Pospi1/Vid primers or Taqman RT

Vinca See chapter 26 and chapter 27

F.o.spp

X Plating

Dipladenia Mandevilla See chapter 26 and chapter 27


Family: Araceae

Genus: Anthurium spp.



Test method

Remarks



* * 100/

1.000 100/

1.000

* Only tissue culture propagated material permitted

CMV Cq and

No X ELISA or PCR

See chapter 26 and chapter 27l



Potyvirus (DsMV) X ELISA See chapter 26 and chapter 27

X.a.dief

X X X X X Plating/ IF/PCR


Ralstonia solanacearum

X X X X X Plating/ IF/PCR


Additional pathogens irrigation water

Quantity ± 0,5 ltr

± 0,5 ltr

± 0,5 ltr

± 0,5 ltr

± 0,5 ltr


X X X X X Plating/ PCR



Family: Asclepiadaceae




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR


Family: Asteliaceae

Genus: Cordyline


plants 3 SEE1 SEE2 EE E

Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR



Family: Asteraceae (Compositae), with the exception of Dahlia, Chrysanthemum spp. (Dendranthema spp.)




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR


CVB X X ELISA See chapter 26 and chapter 27

TAV X X ELISA See chapter 26 and chapter 27



Pectolytic Erwinia (chrysanthemi and caratovorum)

X Plating/ IF/PCR

Helichrysum (Bracteantha), Osteospermum, Xerochrysum


CVB

Cq and No

X X X ELISA

Helichrysum (Bracteantha), Xerochrysum, Osteospermum See chapter 26 and chapter 27

TAV X X X ELISA

Helichrysum (Bracteantha), Xerochrysum See chapter 26 and chapter 27

TRSV X ELISA

Aster, Tagetes Echinacea See chapter 26 and chapter 27

BWYV X ELISA

Argyranthemum Achillea Senecio/Pericallis/Cineraria, Gorteria See chapter 26 and chapter 27

CSVd

X X PCR

Argyranthemum, Ageratum, Senecio/Pericallis/Cineraria Sanvitalia See chapter 26 and chapter 27


Family: Asteraceae (Compositae), with the exception of Dahlia, Chrysanthemum spp. (Dendranthema spp.) (SEQUEL)




Test method

Remarks



100/

1.000 100/

1.000

TBRV/BRSV Cq and No X ELISA

Ajania See chapter 26 and chapter 27

A.tum

X PCR Argyranthemum See chapter 26 and chapter 27

ToRSV

Cq and No X ELISA

Echinacea, Helenium See chapter 26 and chapter 27

TSV X ELISA Calendula See chapter 26 and chapter 27

Rhodococcus fascians

X X X

TaqMan PCR/ LAMP PCR

Leucanthemum



Family: Asteraceae (Compositae)

Genus: Dahlia



Test method

Remarks



100/ 1.000

100/ 1.000

AMV Cq and No

X ELISA See chapter 26 and chapter 27




X ELISA See chapter 26 and chapter 27

Pospiviroids (CSVd, PSTVd)

X

Pospi1/Vid primers or TaqMan RT PCR


TSWV Cq and No


INSV X X ELISA See chapter 26 and chapter 27

TSV X X X X X ELISA See chapter 26 and chapter 27


20/200 20/200

DMV complet: DCMV (= DMV-Holland) & DMV (=DMV-Portland)

X* X X X X PCR

** C2 Testing of candidate plants at least 2x (with the exception of seed originated material; this should be tested at least 1x) See chapter 26 and chapter 27

PCR: Nicolaisen, 2003, Plant Disease 87: 945-948 M. Nicolaisen, 2003, Partial molecular characterization of Cahlia mosaic virus and its detection by PCR. Plant Disease 87: 945-948. Publications DCMV: Nicolaisen. M. (2003). Partial molecular characterization of Dahlia mosaic virus and its detection by PCR. Plant Disease 87: 945-948. DMV: Pahalawatta, V., Miglino, R., Druffel, K. B., Jodlowska, A., van Schadewijk, A.R., Pappu, H.R. (2007). Incidence and relative prevalence of distinct caulimoviruses (genus Caulimovirus, family Caulimoviridae) associated with Dahlia mosaic in Dahlia variabilis. Plant Disease 91:1194-1197.

Pectolytic Erwinia (chrysanthemi)

X Plating/ IF/PCR



Family: Asteraceae (Compositae)

Genus: Chrysanthemum spp. (Dendranthema spp.)



Test method

Remarks

Percentage plants 100% 100% 100% 10% 10% 0,5%


100/

1.000 *

100/ 500

*

* Testing every 4 months all propagating material (in vivo)

CVB Cq and No


TAV X X ELISA See chapter 26 and chapter 27



CSVd

X X X X X PCR See chapter 26 and chapter 27

CSNV Cq and

No X ELISA See chapter 26 and chapter 27

CChMVd

X PCR See chapter 26 and chapter 27

Family: Balsaminaceae

Genus: Impatiens



Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No


BaCV X ELISA or PCR











TRV X ELISA or PCR


TSV X ELISA See chapter 26 and chapter 27



Family: Begoniaceae (with the exception of Begonia elatior)




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR




X.a.beg

X X Plating/ PCR



Family: Begoniaceae

Genus: Begonia elatior



Test method

Remarks



TSWV

Cq and No X* ELISA

* Not applicable for plants derived from own company See chapter 26 and chapter 27

INSV X* ELISA

* Not applicable for plants derived from own company See chapter 26 and chapter 27

X.a.beg

X X Plating/ PCR


F.foetens

X 25* 25* 25* 25* Plating/ PCR

See chapter 26 and chapter 27 * Plants per compartment (if possible) deviant or suspicious plants


X Uitplaten/ IF/PCR


Additional pathogens irrigation water

Quantity ± 0,5 ltr

± 0,5 ltr

± 0,5 ltr

± 0,5 ltr

± 0,5 ltr

F.foetens







Family: Berberidaceae

Genus: Epimedium



Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No






ToMV-D-03 X X ELISA See chapter 26 and chapter 27

TMV-WU1 X X ELISA See chapter 26 and chapter 27





Family: Boraginaceae




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR



Family: Brassicaceae (Cruciferae)




Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No











TRV X ELISA or PCR



TBRV/BRSV Cq and

No X ELISA Aubrieta See chapter 26 and chapter 27

TuYMV – related virus

X ELISA Erysimum See chapter 26 and chapter 27


Family: Buxaceae

Genus: Pachysandra



Test method

Remarks



100/

1.000 100/

1.000

AMV Cq and No





X