synthesis, electrochemistry, imaging & analytical systems ... · synthesis of libraries of...
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Labex IPGG - FPGG 29 rue d’Ulm 75005 Paris 01 40 79 51 56 / [email protected] http://www.ipgg.fr
SYNTHESIS, ELECTROCHEMISTRY, IMAGING & ANALYTICAL SYSTEMS FOR DIAGNOSIS
Dr Fethi Bedioui
SYNTHESIS: Development and use in automated synthesis of recyclable supported catalysts for the Huisgen reaction and synthesis of libraries of molecules with or without solvent , Study of reactivity and bis-triazole regioselective synthesis , Identification of ligands for proteins by screening on small molecules chip ELECTROCHEMISTRY: Electrochemical sensors for biological system: Multi sensor arrayed platform for screening biological markers, Molecular materials for electroanalysis and electrocatalysis, Microelectrochemical patterning of surfaces using scanning electrochemical microscopy, Label-free electrochemical detection of microRNAs IMAGING: Preparation of targetedoptical and MR imaging agents, Development of molecular magnetic resonance imaging MRI methods & functional imaging methods for the characterization and therapeutic follow-up of cancer in preclinics ANALYTICAL SYSTEMS: Development of miniaturized total analysis systems for applications ranging from environmental control to in-vitro medical diagnostics, Characterization of new nano-supports and selective objects : peptide nanotubes, aptamers, nanobodies, Design of original biocompatible nano-objects and quantitative characterization of their non-covalent interactions
Abdelilah AMAR Chemical Engineer CNRS Analy&cal Electrochemistry
Fethi BEDIOUI Senior Researcher CNRS Analy&cal ElectroChemistry Miniaturized Devices
Bich-‐Thuy DOAN CNRS researcher Preclinical MRI and MRS
Fanny d’ORLYÉ Associate professor Chimie ParisTech Analy&cal Chemistry -‐ Miniaturized Systems
Hervé GALONS Professor Paris Descartes Medicinal Chemistry
ChrisGan GIRARD Associate Professor Chimie ParisTech Medicinal Chemistry, Molecular Screening Miniaturized Systems
Sophie GRIVEAU Associate professor Chimie ParisTech Electrochemical microsensors Micro electrochemistry
Mathieu LAZERGES Associate Professor Paris Descartes Electrochemical DNA-‐Biosensors Topical Anesthesia and Skin Regenera&on
Cyrine SLIM Associate Professor Chimie ParisTech Electrochemical microsensors Micro electrochemistry
Anne VARENNE Professor Chimie ParisTech Analy&cal Chemistry Miniaturized devices – Diagnos&cs
FOCUS on our projects
1. Design of a lab-on-a-chip integrating an aptamer-based molecular capture as innovative sample pretreatment strategy for the online identification and quantitation of trace amounts of emerging contaminants in water
Y. LADNER, F. d’ORLYE, C. PERRARD, B. Da SILVA, C. GUYON, M. TATOULIAN, S. GRIVEAU, F. BEDIOUI & A. VARENNE. Surface functionalization by plasma treatment and click chemistry of a new family of fluorinated polymeric materials for microfluidic chips. Plasma Process Polym, DOI : 10.1002/ppap.201300120. Y. LADNER, F. d’ORLYE, C. PERRARD, B. Da SILVA, C. GUYON, M. TATOULIAN, S. GRIVEAU, F. BEDIOUI & A. VARENNE. . Surface functionalization of COC microfluidic materials by plasma and click chemistry processes. Plasma Process Polym. 10 (2013) 959-969. S. GRIVEAU & F. BEDIOUI. Overview of significant examples of electrochemical sensor arrays designed for the detection of nitric oxide and relevant species in biological environement. Anal. Bioanal. Chem. 405 (2013) 3475-3488. M. GIRARDOT, F. d’ORLYE, S. DESCROIX, A. VARENNE . Aptamer-conjugated nanoparticles : preservation of targeting functionality demonstrated by microchip electrophoresis in frontal mode . Anal. Biochem, 435 (2013) 150-152. M. GIRARDOT, HY LI, S. DESCROIX, A. VARENNE. Binding parameters between an aptamer and its target by frontal analysis continuous microchip electrophoresis (FACMCE): a comprehensive study of buffer composition and thermal treatment in the case of lysozyme. Chromatographia, Volume 76 (2013) 305-312 D. QUINTON, A. GIRARD, L. TO THI KIM, V. RAIMBAULT, L. GRISCOM, F. RAZAN, S. GRIVEAU & F. BEDIOUI. On-chip multi electrochemical sensor array platform for simultaneous screening on nitric oxide and peroxynitrite. Lab On Chip 11 (2011) 1342-1350. M. GIRARDOT, H.-YI LI, S. DESCROIX, A. VARENNE. Determination of binding parameters between lysozyme and its aptamer by frontal analysis continuous microchip electrophoresis (FACMCE). J. Chromatogr. A, 1218 (2011) 4052-4058. B. TESTE, F. KANOUFI, S. DESCROIX,P. PONCET, T. GEROGELIN, JM. SIAUGUE, J. PETR, A. VARENNE, MC. HENNION. Kinetic analyses and performance of a colloidal magnetic nanoparticle based immunoassay dedicated to allergy diagnosis . Anal. Bioanal. Chem, 400 (2011)3395-3497 B. TESTE, F. MALLOGI, JM. SIAUGUE, A. VARENNE, F. KANOUFI, S. DESCROIX. Microchip Integrating Magnetic Nanoparticles for Allergy Diagnosis. Lab on Chip 11 (2011) 4207-4213
[email protected] [email protected]
[email protected] [email protected]
[email protected] [email protected] [email protected] [email protected]
[email protected] [email protected]
LABORATORY PRESENTATION
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FOCUS ON MICROFLUIDICS PROJECTS
CONTACT DETAILS HIGHLIGHTED PUBLICATIONS
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Injec&on(Concentra&on(Separa&on(
Detec&on(
Sample'reservoir'
Electrolyte'reservoir'
Working'electrode'
Auxiliary'electrode'
Reference'electrode'
Fluorescence* Electrochemical*
Mass**spectrometer*
Ligand*confinement*zone*
Simple*lab:on:a:chip*New*materials* Global*/*local*func@onaliza@on*
Sensi@ve*detec@on*
An*original*molecular*capture*by*APTAMERS*
Cu (I)solvent
Si
Spacer
N3
O O Si
Spacer
N
O O
NN
2. Design of microchips for the selective electrochemical detection of nitrosothiols (RSNO) in biological fluids 3. Synthesis of small molecule librairies in continous flow micro reactors
Selective Electrochemical detection of NO by a selective microelectrode positionned at the end
of the microchannel
Microchip for electrokinetic separation and electrochemical detection of low and high molecular weight RSNOs Principle of RSNO detection
Cu2+ and reducing agent RSNO NO + RS-‐Cu2+