Supplementary Materials

Figure S1 – (a) ISDD Model simulations for TNP in 6-well and 96-well plates. (b) Corresponding time-weighted average (TWA) factor [insert: lower incubation times]. (c, d) Time-weighted average dosage (TWAD) for 2 and 24 hours against the absolute exposure for (c) 96-well plate format and (d) 6-well plate format (different units are used for TWAD to fit into plot area).

0 5 10 15 20 250

0.2

0.4

0.6

0.8

1

1.2

6-well plate

96-well plate

Incubation Time

Fra

ctio

n D

epos

ited

A

0 5 10 15 20 250

5

10

15

20

25

f(x) = 0.9811875 x − 5.0185R² = 0.999654562363468

f(x) = 0.998717142857143 x − 1.80924R² = 0.999999917869009

6-well plateLinear (6-well plate)

Incubation Time (hours)

TW

A F

acto

r

B

0 1 2 3 40

0.5

1

1.5

2

2.5

f(x) = 0.0463845 x² + 0.00108664 xR² = 0.999999994323281

f(x) = 0.134356 x² + 0.00854603 x

Incubation Time (hours)

TWA

Fact

or

0 100 200 300 4000

50

100

150

200

250

96-well plate

2 hrs (µg/cm²)

24 hrs (x10² µg/cm²)

Absolute Exposure (µgml-1)

TW

AD

C

0 50 1001502002503003504000

5

10

15

20

256-well plate

2 hrs (µg/cm²)

24 hrs (x 10² µg/cm²)

Absolute Exposure (µgml-1)

TW

AD

D

Table S1: Some examples of the TWA factor and TWAD values used.

Format 6-well plate 96-well plate

Time TWA FactorTWAD

(AE: 50 ugcm-2) TWA FactorTWAD

(AE: 100 ugcm-2)0.25 0.0094 0.049474 0.0032 10.5 0.036 0.189474 0.0121 3.781251 0.1409 0.741579 0.0475 14.843752 0.5571 2.932105 0.1877 58.656254 2.1834 11.49158 0.7465 233.28138 6.1806 32.52947 2.9769 930.281312 10.1771 53.56368 6.6086 2065.18816 14.1724 74.59158 10.6082 3315.06320 18.1663 95.61211 14.6077 4564.90624 22.157 116.6158 18.6011 5812.844