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Supplementary files Rotaru et al., EES 2013,
Supplementary figures
Figure S1. Work flow of metatranscriptomics analysis
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Figure S2. (a) Scatter plot comparison of gene expression levels in replicate co-
cultures and (b) digester aggregates. An example of how the median log2 RPKM value
was calculated for c) G. metallireducens and d) M. harundinacea in co-culture. Each data
point on the scatter plot represents an individual gene and is plotted based on its
expression level.
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Table S1. Assembly statistics of reads from digester and G. metallireducens – M.
harundinacea co-cultures. Digester and coculture sequences were assembled with mers
of 15 and 21 nucleotide length, respectively.
Table S2. List of genes belonging to Methanosaeta concilii and Geobacter
metallireducens and their normalized expression levels. Data is representative of
duplicate reactor samples.
Experiments Template genomes
Total number of reads with mer hits
Unique reads assigned
Methanosaeta concilii
8.18 ×105 Digester
Geobacter metallireducens
6.82×106
2.36 ×105
Co-culture Methanosaeta harundinacea
1.04×106
Geobacter metallireducens
4.47×106
1.65×106
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Table S3. List of genes belonging to Methanosaeta harundinacea and Geobacter
metallireducens and their normalized expression levels. Data are representative of
triplicate co-cultures.
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