supplementary figure 1. a b b12 (nm) 500 500 500 500 0.15 0.15 0.15 0.15 - - - folate (µm) 6 15 30...
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![Page 1: Supplementary Figure 1. A B B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - - Folate (µM) 6 15 30 60 6 15 30 60 - 30 60 Control * * ** * * * * * B12 (nM)](https://reader036.vdocuments.us/reader036/viewer/2022082505/56649d9d5503460f94a863f7/html5/thumbnails/1.jpg)
Supplementary Figure 1.
A
B
B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - -
Folate (µM) 6 15 30 60 6 15 30 60 - 30 60
Control
Se-ries1
50
100
150
To
tal
Ch
ole
ster
ol
(%)
**
***
*
****
Se-ries1
0
1
2
3
Ho
mo
cyst
ein
e (u
M)
*
**
**
**
**
**
B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - -
Folate (µM) 6 15 30 60 6 15 30 60 - 30 60
Control
*
![Page 2: Supplementary Figure 1. A B B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - - Folate (µM) 6 15 30 60 6 15 30 60 - 30 60 Control * * ** * * * * * B12 (nM)](https://reader036.vdocuments.us/reader036/viewer/2022082505/56649d9d5503460f94a863f7/html5/thumbnails/2.jpg)
Supplementary Figure 2: Bisulphite-Pyrosequencing Amplicon
B LDLR
A SREBF1
TGATTTTGCTTTGAGGGAGAGGAAACCG1CCCTTCTGTTTGTTCAACTCCTTCTCCTAAGGGGAGAAATCAATATT
TACG2TCCAGACTCCAGGTATCCG3TACAATTGATTTTTCAGATGTTTATACTCAGCCAAAGGCG4GGATCCCACAA
AACAAAAAATATTTTTTTGGCTGTACTTTTGTGAAGATTTTAT
CCCTGCCTGGGCTCTGATGCAAATGCACTGGCTTCAAGCCAGGCCTCCAGTGCG4AGGTTGCG3CCTA
CCCCG2GCAACAAGCACACCAGGACTGCTAGTAACAGGGCATCTCAAGCAGCCG1AGTGGAGCCCCAG
TTTCTAAAGCCTGCACAGACCTCT
Flanking primer sequences for BS-Pyrosequencing amplicon
CpG sites
Sequencing Primer
![Page 3: Supplementary Figure 1. A B B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - - Folate (µM) 6 15 30 60 6 15 30 60 - 30 60 Control * * ** * * * * * B12 (nM)](https://reader036.vdocuments.us/reader036/viewer/2022082505/56649d9d5503460f94a863f7/html5/thumbnails/3.jpg)
Location of Illumina 450k probe (cg27407935) and bisulphite
pyrosequencing assay
Location of Illumina 450k probe (cg22971501) and
bisulphite pyrosequencing assay
A
SREBF1
BLDLR
Supplementary Figure 3
![Page 4: Supplementary Figure 1. A B B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - - Folate (µM) 6 15 30 60 6 15 30 60 - 30 60 Control * * ** * * * * * B12 (nM)](https://reader036.vdocuments.us/reader036/viewer/2022082505/56649d9d5503460f94a863f7/html5/thumbnails/4.jpg)
A
B
Supplementary Figure 4
PPARγ binding site
TSS
C/EBPα binding site
LDLR
PPARγ binding sites
TSS TSS
C/EBPα binding sites
SREBF1 TSSTSS
TSS TSS
![Page 5: Supplementary Figure 1. A B B12 (nM) 500 500 500 500 0.15 0.15 0.15 0.15 - - - Folate (µM) 6 15 30 60 6 15 30 60 - 30 60 Control * * ** * * * * * B12 (nM)](https://reader036.vdocuments.us/reader036/viewer/2022082505/56649d9d5503460f94a863f7/html5/thumbnails/5.jpg)
Supplementary Figure 5: Effect of B12 in primary human adipocytes
Control LB NoB50
75
100
125
150
175
To
tal
cho
lest
ero
l (%
)
**
******
Control LB NoB0
1
2
3
Ho
mo
cyst
ein
e (u
M)
****
Control LB NoB0
1
2
3
4
SREBF1
Rel
ativ
e m
RN
A e
xpre
ssio
n
*****
Control LB NoB0
1
2
3
4
SREBF2
Rel
ativ
e m
RN
A e
xpre
ssio
n
******
Control LB NoB0
1
2
3
4
LDLRR
elat
ive
mR
NA
exp
ress
ion
*****
A B C
D E