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upplemental Table 1 Source and authentication of cell lines Cellline Source Authentication LNCaP Dr.Leland W .K.Chung (M DACC,Houston,TX,1992) shorttandem repeat(STR)profile analysis,GRCF 1 ,Jan 2013 M R49F Established in the lab from LNCaP cells shorttandem repeat(STR)profile analysis,GRCF 1 ,Jan 2013 PC-3M -luc (C6) Purchased from CaliperLife Sciences (Hopkinton,MA),July 2011 1 Genetics Resources Core Facility atJohns Hopkins

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Page 1: Supplemental Table 1 Source and authentication of cell lines

Supplemental Table 1

Source and authentication of cell lines

Cell line Source AuthenticationLNCaP Dr. Leland W.K. Chung (MDACC, Houston, TX, 1992) short tandem repeat (STR) profile analysis, GRCF1, J an 2013MR49F Established in the lab from LNCaP cells short tandem repeat (STR) profile analysis, GRCF1, J an 2013PC- 3M- luc (C6) Purchased from Caliper Life Sciences (Hopkinton, MA), J uly 2011

1 Genetics Resources Core Facility at J ohns Hopkins

Page 2: Supplemental Table 1 Source and authentication of cell lines

Supplemental Table 2

Primers used for quantitative real-time PCR

Sequence Sequence 5’ to 3’ Sequence 5’ to 3’Name Forward ReverseLUC CCGCGTACGTGATGTTCACC GAGGATGGAACCGCTGGAGACLU GAGCAGCTGAACGAGCAG CTTCGCCTTGCGTGAGGT

Page 3: Supplemental Table 1 Source and authentication of cell lines

Supplemental Figure S1

PC-3M-luc

4x104 2x104 1x104 5x103 2.5x103 1.13x103

Mea

n Ph

oton

s /

Seco

nd

4.00E+04

2.00E+04

1.00E+04

5.00E+03

2.50E+03

1.13E+03

0.00E+005.00E+061.00E+071.50E+072.00E+072.50E+073.00E+073.50E+074.00E+074.50E+07

PC-3M-luc-C6

Cell number

Cell number

Page 4: Supplemental Table 1 Source and authentication of cell lines

Supplemental Figure S2

-D8 D0 D8

-D8 D0 D80.00E+00

2.00E+09

4.00E+09

6.00E+09

8.00E+09

1.00E+10

1.20E+10

Mea

n Ph

oton

s /

Seco

nd

A

B

-D8 D0 D80

50100150200250300350400450500

Mea

n tu

mor

vol

ume

(mm

3 )

Mea

n Ph

oton

s /

Seco

nd

Mean tumor volume (mm3)

0 100 200 300 400 5000

2000000000

4000000000

6000000000

8000000000

10000000000

12000000000

f(x) = 27759945.10365 x − 2101839865.051R² = 0.916827966962127

x108

Phot

ons/

Seco

nd/c

m2 /

sr

2.5

1.5

0.5

Page 5: Supplemental Table 1 Source and authentication of cell lines

C

DLNP GFP-siRNA

5mg/kgLNP LUC-siRNA

1 mg/Kg

LUC

B-Actin

LNP LUC-siRNA2.5 mg/Kg

LNP LUC-siRNA5 mg/Kg

E

LUC

mRN

A ex

pres

sion

(%

of c

ontr

ol)

LNP GFP-siRNA

5mg/kg

LNP LUC-siRNA

1 mg/kg

LNP LUC-siRNA 2.5 mg/kg

LNP LUC-siRNA

5 mg/kg

0

20

40

60

80

100

120

Rela

tive

Incr

ease

(%)

D0 D3 D5 D80

50

100

150

200

250

300

350

400

450

500 LNP GFP-siRNA 5 mg/kgLNP LUC-siRNA 1 mg/kgLNP LUC-siRNA 2.5 mg/kg

***

D0 D82425262728293031

LNP GFP-siRNA 5 mg/kgLNP LUC-siRNA 1 mg/kgLNP LUC-siRNA 2.5 mg/kgLNP LUC-siRNA 5 mg/kg

Mea

n m

ice

body

wei

ght (

g)

Supplemental Figure S3A B

Mea

n tu

mor

vol

ume

(mm

3 )

D0 D80

100

200

300

400

500

LNP GFP-siRNA 5 mg/kgLNP LUC-siRNA 1 mg/kgLNP LUC-siRNA 2.5 mg/kgLNP LUC-siRNA 5 mg/kg

Page 6: Supplemental Table 1 Source and authentication of cell lines

Supplemental Figure S4

0 5 10 400

20

40

60

80

100

120

LNP LUC-siRNA

LNP CLU-siRNA

**

Cell

grow

th (%

of c

ontr

ol)

ENZ μmol/L

**

LNCaP


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