Supplemental Figure S1.

G GA M K

GGGG LDF I I

Supplemental Figure S2.

MPALGCCVD--ATVSPPLGYAFSSDSSLPTPEFFSSGVPPMTNAAAG-----HSHWSPDLSSALYRVD 61MPALGCCVDA-AVVSPPLSYAFSRDSSLPAPEFFASGVPPTNSAAA-------SHWSPDLSSALYGVD 60MPALACVDTSFVPPAYAFSDTAG--------DVFIPASSPTSAAVVV------DRWSPSLSSSLYRID 54MPALTCFVDGAAALLHPPGYALAGDFTLPLPFTFSAAATITDDADATAVEDSNSIWSPSLSSKLFRID 68

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1 MPALAFVDTP--------IDTFS--------SIFTPSSVSTAVVDGS------CHWSPSLSSSLYRID 46

GWGAPYFSVNSSGDISVRPHGTDTLPHQEIDLLKVAKKAADPKHLGGLGLQMPLVVRFPDILKNRLET 129GWGAPYFSVNSNGDISVRPHGTDTLPHQEIDLLKVVKKASDPKNSGGLGLQLPLVVRFPDVLKNRLES 128GWGAPYFIANSSGNISVRPHGSETLPHQDIDLLKIVKKVTGPKSSGGLGLQLPLIVRFPDVLKNRLEC 122GWGFPYFGVNAAGDISVRPHGSATMSHQEIDLLKVVKKASDPKCCGGLGLQLPLVVRFPDVLKDRLES 136GWGAPYFAANSSGNISVRPHGSNTLPHQDIDLMKVVKKVTDP---SGLGLQLPLIVRFPDVLKNRLEC 111

====================LQSAFDMAVRSRGYEAHYQGVYPVKCNQDRFVVEDIVKFGSPYRFGLEAGSKPELLLAMNCLSKGSAD 197LQSAFDLAVHSQGYGAHYQGVYPVKCNQDRFVVEDIVKFGSPFRFGLEAGSKPELLLAMSCLCKGSAE 196LQSAFDYAIKSQGYDSHYQGVYPVKCNQDRFVVEDIVKFGSSFRFGLEAGSKPEILLAMSCLCKGSPD 190IHAAFDGAIQLQGYESHYQGVYPVKCNQDRYIVEDIVEFGSSFRFGLEAGSKPELLLAMSCLCKGNRE 204LQSAFDYAIQSQGYDSHYQGVYPVKCNQDRFIIEDIVEFGSGFRFGLEAGSKPEILLAMSCLCKGNPE 179

ALLVCNGFKDTEYISLALIARKLLLNTVIVLEQEEELDLVIDISRKMAVRPVIGLRAKLRTKHSGHFG 265GLLVCNGFKDAEYISLALVARKLMLNTVIVLEQEEELDLVIDISHKMAVRPVIGLRAKLRTKHSGHFG 264AFLVCNGFKDAEYISLALLGRKLALNTVIVLEQEEELDLVIELSQKMNVRPVIGLRAKLRTKHSGHFG 258AFLVCNGFKDSEYISLALIARKLALNTVIVLEQEEELDMVVEISNKLCIRPVIGVRAKLKTKHSGHFG 272AFLVCNGFKDSEYISLALFGRKLELNTVIVLEQEEELDLVIDLSQKMNVRPVIGLRAKLRTKHSGHFG 247

--STSGEKGKFGLTTTQILRVVKKLEGSGMLDCLQLLHFHIGSQIPTTELLADGVGEATQIYSELVRLGA 333STSGEKGKFGLTTTQIVRVVKKLEESGMLDCLQLLHFHIGSQIPSTGLLADGVGEAAQIYCELVRLGA 332STSGEKGKFGLTTTQIVRVVRKLRQSGMLDCLQLLHFHIGSQIPSTSLLSDGVAEAAQLYCELVRLGA 326ATSGDKGKFGLTTIQILHVVKKLEQLDMLDCLQLLHFHIGSQIPTTELLADGVREASQIYCELLRLGA 340STSGEKGKFGLTTVQILRVVRKLSQVGMLDCLQLLHFHIGSQIPSTALLSDGVAEAAQLYCELVRLGA 315

------------GMKFIDIGGGLGIDYDGSKSSDSDVSVAHGIEEYASAVVQAVQFVCDRKGVKHPVICSESGRAIVSHH 401GMKFIDIGGGLGIDYDGTKSCDSDVSVGYGIQEYASAVVQAVQYVGDRKGVKHPVICSESGRAIVSHH 400HMKVIDIGGGLGIDYDGSKSGESDLSVAYSLEEYAEAVVASVRVVCDRSSVKHPVICSESGRAIVSHH 394QMKVLDIGGGLGIDYDGSKSGDSDESVAYGLEEYAAAVVHAVKYVCDRKNVKHPVICSESGRAIVSHH 408HMKVIDIGGGLGIDYDGSKSGESDLSVAYSLEEYAAAVVASVRFVCDQKSVKHPVICSESGRAIVSHH 383

SIMIFEAVSASTTNVSP-QLSSGGLQSLAETLNEDARADYRNLSAAAVRGEYDTCLLYSDQLKQRCVE 468SILIFEAVSASSTHVSSSHLSSGGLQSMAETLNEDALADYRNLSAAAVRGEYETCVLYSDQLKQRCVE 468SVLIFEAVSADKP--MVHQATPGDIQFLLEGN-EEARANYEDLYAAVMRGDHESCLLYVDQLKQRCVE 459SILIFEASGASTNTAPS--LSSIELQYLGEGLSEEALADYQNISAATLRGEYEACLLYTEQFKKRCVE 474SVLIFEAVSAGQ-----QHETPTDHQFMLEGYSEEVRGDYENLYGAAMRGDRESCLLYVDQLKQRCVE 446

QFNDGSLDIEQLAAVDSICDLVSKAIGVSDPVRAYHVNLSVFTSIPDFWAFGQLFPIVPIHRLDEKPT 536QFKEGSLGIEHLAAVDSICDFVSKAMGAADPVRTYHVNLSIFTSIPDFWAFGQLFPIVPIHRLDEKPA 536GFKEGVLSIEQLASVDGLCEWVLKAIGASDPVHTYNINLSVFTSIPDLWGIDQLFPIVPIHKLDQRPG 527EFKQGTLGIEQLAAVDGLCDLITETIGVKDPVKKYHVNLSVFTSVPDFWGINQLFPIVPIHRLDEKPT 542GFKEGSLGIEQLAGVDGLCEWVIKAIGASDPVLTYHVNLSVFTSIPDFWGIDQLFPIVPIHKLDQRPA 514

MRGILSDLTCDSDGKVDKFIGGESSLPLHDLGSNGGDGGRYYLGMFLGGAYEEALGGLHNLFGGPSVV 604VRGILSDLTCDSDGKVDKFIGGESSLPLHELGSNG-DGGGYYLGMFLGGAYEEALGGLHNLFGGPSVV 603ARGILSDLTCDSDGKINKFIGGESSLPLHELDKNG-SGGRYFLGMFLGGAYEEALGGVHNLFGGPSVV 594ARGILSDLTCDSDGKIDKFIGGESSLPLHEMEGHG---GGYYLGMFLGGSYEEALGGLHNLFGGPSVV 607ARGILSDLTCDSDGKINKFIGGESSLPLHEMDNNGCSGGRYYLGMFLGGAYEEALGGVHNLFGGPSVV 582

RVLQSDSPHSFAVTRSVPGPSCADVLRAMQFEPELMFETLKHRAEESFDQEEEIEDKGLAFE------ 666RVVQSDSAHSFAMTRSVPGPSCADVLRAMQHEPELMFETLKHRAEEFLEQED---DKGLAVE------ 662RVSQSDGPHSFAVTRAVPGQSSADVLRAMQHEPELMFQTLKHRAEEMMNNNNKACGDKGNDK-----L 657RVLQSDGPHGFAVTRAVAGSSCADVLRVMQHEPQLMFETLKHRALEFCGQHD--DDSVVNAG------ 667RVLQSDGPHGFAVTRAVMGQSSADVLRAMQHEPELMFQTLKHRAEEPRHTKGGSEGENEEEEEDDEFN 650

SLISGLAQSFHNMPYLVAPSSCCFTAATAKGGGY-YYYNEDNAAD------CATAEDEIWSYCTA 724SLASSVAQSFHNMPYLVAPSSCRFTAATDNNGGYNYYYSDENAAD------SATGEDEIWSYCTA 721 (86%)VVASCLAKSFNNMPYLSMETS--TNALTAAVNNLGVYYCDEDVYDY-----ISA----------- 711 (71%)VLANSLAQSFDNMPYLVSSTTCCLNALTNNNGFY-YCSGDDFSADTVSVATSVAGEDENWSY--- 728 (69%)NVAASLDRSFHNMPYLATEQASPSNSLSAAISNLGFYYCDEAAAGG-----GGKGKDENWSYFG- 702 (68%)

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

CaADC1 Tobacco ADC

Arabidopsis ADC2 Garden pea ADC

Arabidopsis ADC1

A

Leaf

Stem

Roo

t

Flow

er

Gre

en fr

uit

Red

frui

t

CaADC1

rRNA

CaADC1

rRNA

0 1 5 10 15 20 25h

Salicylic acid

B

CaADC1

rRNA

0 1 5 10 15 20 25 hMethyl jasmonate

CaADC1

rRNA

0 1 5 10 15 20 25h

EthyleneC

Supplemental Figure S3.

D

35S:EV(OD600=0.2)

35S:CaADC1 K154A

(OD600=0.2)35S:CaADC1(OD600=0.2)

35S:CaADC1K154A /

35S:avrBsT(OD600=0.05)

35S:avrBsT(OD600=0.2)

35S:avrBsT(OD600=0.05)

35S:CaADC1 / 35S:avrBsT

(OD600=0.05)

35S:CaADC1K154A / 35S:BAX

(OD600=0.05)35S:BAX

(OD600=0.2)35S:BAX

(OD600=0.05)

35S:CaADC1 / 35S:BAX

(OD600=0.05)

AB

25

20

15

10

5

0

Con

duct

ivity

(μS

cm-1

)

Supplemental Figure S4.

0 24 48Hours after infiltration

35S:EV (OD600=0.2)

35S:CaADC1 (OD600=0.2)

35S:CaADC1 K154A (OD600=0.2)

35S:avrBsT (OD600=0.05)

35S:CaADC1 / 35S:avrBsT (OD600=0.05)

35S:CaADC1 K154A / 35S:avrBsT (OD600=0.05)

35S:avrBsT (OD600=0.2)

35S:CaADC1 / 35S:BAX (OD600=0.05)

35S:BAX (OD600=0.05)

35S:CaADC1 K154A / 35S:BAX (OD600=0.05)

35S:BAX (OD600=0.2)

aa a a a a a a aa a a aa a a bb bc

dd

d d

c

b b b b baaa

Cell death (+) (+) (+) (+++)

Cell death (+) (++) (+) (+++)

Cell death (-) (-) (-)

35S:EV(OD600=0.2)

35S:CaADC1 K154A

(OD600=0.2)35S:CaADC1(OD600=0.2)

35S:CaADC1K154A /

35S:avrBsTC222A

(OD600=0.05)

35S:avrBsTC222A

(OD600=0.2)

35S:avrBsTC222A

(OD600=0.05)

35S:CaADC1 / 35S:avrBsT

C222A(OD600=0.05)

35S:avrBsT(OD600=0.2)

B

35S:avrBsT C222A (OD600=0.05)

35S:CaADC1 / 35S:avrBsT C222A (OD600=0.05)

35S:CaADC1 K154A / 35S:avrBsT C222A (OD600=0.05)

35S:avrBsT C222A (OD600=0.2)

C

25

20

15

10

5

0

Con

duct

ivity

(μS

cm-1

)

Supplemental Figure S5.

35S:EV (OD600=0.2) 35S:CaADC1 (OD600=0.2)

35S:avrBsT (OD600=0.2)35S:CaADC1 K154A (OD600=0.2)

0 24 48Hours after infiltration

a

b

b

aa a a a a a a aa aa aa a a a aa a a

Cell death (-) (-) (-) (+++)

Cell death (-) (-) (-) (-)

AvrBsT:cMyc

AvrBsT C222A:cMyc

CaADC1:HA

+ +

+ +--

- -

IP: cMyc IP: HA

α-cMyc

α-HA

CBB

CaADC1:HA

-

-

AvrBsT:cMyc /AvrBsT C222A:cMyc

+- - +-

- +

- + +

+ +--

- - -

-+- - +-

- +

-

A

0 1 2Asp Glu Asn Ser Gln Gly His Arg Thr Ala GABA Pro Tyr Val Ile Leu Phe Trp Lys

35S:avrBsT (OD600=0.2)35S:CaADC1 (OD600=0.2)35S:EV (OD600=0.2)

6

5

4

3

2

1

0 0 1 2 0 1 2 0 1 2 0 1 2 0 1 20 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 dai

Supplemental Figure S6.

Amin

o ac

id (

μm

ol g

-1FW

)

aaaa

aa

a

a

a

a

a

a

a

aa

a

a

a

aaa aa

b

b

aa

aaa

a

aa

aa

a

aa

bb

b

bb

c

c

aaa

a

a

aaaa aa aa

aaaa

b bb

b

b

bb

bc

a

aaa aabb

b

c

caaa

a a

a

aa

a

a

a

a

a

a

aaa

bb

b

a

aa

b

b

c

aaa aaaaaa aaa aa

aaa

a

aaa aaa

aa aaa aaa

b

b

b

bb

c

a aaa aaa aa

a

aaaaaaaaaaaaaaa

Visible UV

TRV:

00

cfu mL-1

107

108

Xcv Ds1(EV)

Xcv Ds1(avrBsT)

TRV:

CaA

DC

1

Visible UV

Xcv Ds1(EV)

Xcv Ds1(avrBsT)

Xcv Ds1(EV)

Xcv Ds1(avrBsT)

Xcv Ds1(EV)

Xcv Ds1(avrBsT)

Supplemental Figure S7.

3

2

1

0

3

2

1

0

Asp Glu Asn Ser Gln Gly His Arg Thr Ala GABA Pro Tyr Val Ile Leu Phe Lys0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 0 1 2 dai

TRV:00 TRV:CaADC1

Am

ino

acid

(μ

mol

g-1

FW)

Xcv Ds1 (EV)

Xcv Ds1 (avrBsT)

Supplemental Figure S8.

*****

*

*

*

*

*

*

*

*

*

* * * ** * *

8

6

4

2

0

Log

cfu

cm-2

0 8 16 24 0 8 16 24

Hours after incubation

Xcv Ds1 (EV) Xcv Ds1 (avrBsT)

Mock 1mM Alanine 10mM Alanine 1mM GABA 10mM GABA

Supplemental Figure S9.

aaa a a

a a a a a

a a a a a

a a a a a

a a a a a

a a a a a

a a a a a

a a a a a

GABA

H2O2

Cell death & defense response

Arginine

Agmatine

Putrescine

Spermidine

Spermine

CaADC1

AvrBsT

Supplemental Figure S10.

NO

SA

Pyrroline