Supplemental Figure 1

FLAG/Luc/#1 FLAG/Keap1/#1

A

VimentinFLAG/Luc/#1 FLAG/Keap1/#1

B

A

C

0

5,000,000

10,000,000

15,000,000

20,000,000

25,000,000

Nrf2 shRNA/#2

Rel

ativ

e In

tens

ity

Control

GSH

Nu

mb

er

x 1

0

Levels of ROS

B

Nrf2 shRNA/#2 Control shRNA

Supplemental Figure 2

C400

Ce

ll N

um

be

r Control Nrf2 shRNA

Supplemental Figure 3

Con

trol

shR

NA

pAKT

AKT

GAPDH

Cyclin D1

Nrf

2 sh

RN

A #

2

C-Jun

Supplemental Figure 4

Control lentivirus shRNALentivirus Nrf2 shRNA clone 3

Lentivirus Nrf2 shRNA clone 6

Cel

l N

um

ber

Days

Con

trol

A549 cells

Lentivirus Nrf2 shRNAclones

C

lone

3

Nrf2

GAPDH

B

Clo

ne 6

Supplemental Figure 5

Control shRNA Nrf2 shRNA

5 ng/ml TGF- SB431532 + TGF-

0 hr 24 hr 24 hr

Supplemental Figure 6

Nrf2 siRNA in pSilencer

Control siRNA in pSilencer

O hr 24hr

A

C

Control retrovirus shRNA

Retrovirus Nrf2 shRNA #2

24 hrs

B

24 hrs + TGF- 24 hrs TGF- + SIS3

Supplemental Figure 7

Supplemental Figure 8

“A” side media ports

Cell loading port

“B” side media ports

•6 micron posts

•8 micron posts

•10 micron posts

•12 micron posts

Micro-Taur schematic diagram

Insert illustrating Post Region (12 micron)

Post-containingchambers

0

500

1000

1500

2000

2500

Nrf2-shRNA #2

Supplemental Figure 9

TG

F-

1 pg

/ml

Control shRNA

TGF β TGF β

R-Smad

PPMH1 MH2

R-Smad

MH1 MH2Phosp

hata

ses

Slug

Slug

E-Cadherin

E-Cadherin

Smad target genes

Nrf2

pR-Smad Smad4

Smad target genespR-Smad Smad4

R-Smad

P PPP

MH1 MH2

Phosp

hata

ses

ROS

Loss of Nrf2 expression

ROS

Nrf2 Nrf2

R-Smad

P PPP

MH1 MH2

R-Smad

P PPP

MH1 MH2

Supplemental Figure 10

A B

Smad target genespR-Smad Smad4