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Direct Diffusion-based Parcellation of the Human Thalamus

Vinod Kumar1,2Wolfgang Grodd11Department of Psychiatry, Psychotherapy and Psychosomatics, University Hospital, Aachen, RWTH Aachen University, Aachen, Germany; JARA-Translational Brain Medicine, Germany 2Graduate School of Neural & Behavioral Sciences, International Max Planck Research School, Tbingen, GermanyCorresponding author: Wolfgang GroddProf. Dr. med. Dipl.-Biol. Department of Psychiatry, Psychotherapy and Psychosomatics Rheinisch-Westflische Technische Hochschule (RWTH) Aachen Pauwelstr. 30 D-52074 Aachen, Germany Tel.: +49 241/8037159 FAX: +49 241/8082401 E-Mail: wgrodd@ukaachen.deMale Left (90%)voxelCoGCoGCoGcluster #2 mmxyz1 (PO)38-15.92-30.642.573 (LP)48-18.64-21.792.086 (AN)11-1.78-4.1410.547 (MED)191-3.87-16.778.2513 (LA)18-16.03-10.802.57Morel 4voxelCoGCoGCoGname2 mmxyzAnterior 12256.25-12.3412.02Lateral 342912.55-14.366.95Medial25016.96-17.296.22Posterior366116.04-27.275.23Oxford 7voxelCoGCoGCoGname2 mmxyzPrim. motor cortex71-16.88-21.315.09Sensory cortex102-18.10-22.673.36Occipital cortex30-20.13-31.752.78Pre-frontal cortex737-9.18-14.586.61Pre-motor cortex215-16.11-17.076.99Post. parietal cortex406-19.05-27.253.69Temporal cortex749-9.31-22.529.56Table S1: Summary of the size and spatial location (number of voxels) and center of Gravity (CoG) in MNI 2mm space of of five male clusters of the left hemisphere surviving at 90% probability level and thalamic structures of four major nuclei groups (Morel 4) derived from the atlas of Morel (2007) and seven thalamic areas derived from the Oxford connectivity atlas [Johansen-Berg et al.. 2005]. MALE LeftMALE Thr 90 CoGregionvolumedistanceoverlapoverlapinsidecluster # voxel 2 mmOxford 7namevoxelmmvoxelspercentpercent1 (PO)386Post. parietal cortex4063.4256.265.83 (LP)482Sensory cortex1023.93635.375.06 (AN)114Pre-frontal cortex73712.600.00.07 (MED)1914Pre-frontal cortex7375.0669.034.613 (LA)185Pre-motor cortex21510.083.744.4cluster # voxel 2 mmMorel 4namevoxelmmvoxelspercentpercent1 (PO)384Posterior7633.4385.0100.03 (LP)482Lateral73511.1435.8589.586 (AN)111Anterior 3408.8102.990.97 (MED)1913Medial5573.015026.978.513 (LA)182Lateral 7358.991.250.0cluster # voxel 2 mmMorel 29namevoxelmmvoxelspercentpercent1 (PO)3821PuM4504.5368.094.73 (LP)4830VPI645.62437.550.06 (AN)113AV1924.9105.290.97 (MED)19112MD3031.411237.058.613 (LA)1829VM86.5337.516.7Table S2: Anatomical correspondence of five selected clusters (# 1. 3. 6. 7. 13) in respect to the closest distance of their center of gravity (CoG) and the maximal spatial overlap given in number of voxels and percentage with the four major thalamic regions and 29 nuclei of the atlas of Morel (Morel 4 and Morel 29) and seven regions of the Oxford thalamic atlas (Oxford 7).Subcort.connect.MaleleftMalerightFemaleleftFemalerightSignif. * (p< 0.05)Hemisph.GenderMeanSD# subj.MeanSD# subj.MeanSD# subj.MeanSD# subj.M/F InteractionM/FCluster #1 (PO)Acc63870929797836295997492854549925.594.282.337Amy18839732188212321301413120345730.500.503.726BS323132604832324731495331.000.346.569Cau1622503211386321531693115515231.361.321.646Den102820089146228708106722688124225818.437.740.852Hip65463260393210210631534831.018.047.225Pal20721132226151322343363130041031.340.604.397Put442432606832553931636931.187.627.521Red155123922411041179238551043212446271322.078.002.399mean435452359562cluster #3 (LP)Acc3946811033769696548951769097914.944.752.038Amy8451079257101000273714073048471530.945.447.014BS16432153321433115331.519.068.213Cau59186332773886293124533132928431.402.488.005Den688767321680193629440577291137151225.001.542.091Hip130914752612771397315255243170076231.731.617.001Pal703032787832502331592631.201.047.922Put82383213511032552731742931.000.086.001Red867132430122114072711201221311354197825.302.832.451mean540692394538cluster #6 (AN)Acc29370032243240321852653216414029.628.848.186Amy726117531215234321711883216916231.021.022.009BS117270313123683262523227630031.000.855.356Cau112715113210513132382232221331.000.000.000Den160815688110015891417182175141736197918.449.418.269Hip105413773273643213714432674230.000.001.000Pal149336321601803228193221533331.031.055.469Put233281322411863263513222531231.041.063.030Red1308169927480568306737253052954226.005.045.131735378326342378cluster #7 (MAD)Acc140353326142321684103116639631.377.3950.354Amy6848326039328212131585831.049.339.745BS10532713282318231.002.015.153Cau17332152321443115331.132.023.042Den6156043061010473039730630694115029.282.265.688Hip351232298323620317726431.434.297.331Pal186321663217 3120931.686.006.544Put332732332632321931301331.633.665.642Red57110324568324655317110931.599.130.694mean1109789127cluster #13 (LA)Acc3251020456318150495393798007.183.626.090Amy4421039820572035397331559564722.507.283.108BS301832132863221531281531.000.000.000Cau31138931591556231601163113010531.068.025.000Den1155138226651114310796840261091202710.694.135.866Hip556156150005001076865488120.284.061.115Pal1510323645133218103121327231.000.143.159Put35223217723729301431989631.000.105.083Red1379183063951071611191277261234170818.130.057.255mean472286410491overall mean461406370450Table S3: Subcortical connectivity. Mean number and standard deviation (SD) of streamlines to 9 different subcortical areas from the five thalamic clusters (C1. C3. C6. C7 and C13) for the males and females group.CorticalMaleMaleFemaleFemaleSignificance * (p< 0.05)connectionsleftrightleftrightHemisphereGenderMeanSD# subj.MeanSD# subj.MeanSD# subj.MeanSD# subj.L/R Inter-actionM/FCluster #1 (PO)546578473325488110556325628617134325792016252310.5690.4400.665Cluster #3 (LP)530451397632592901286532575311012932574149433310.0450.6130.037cluster #6 (AN)3750510594323424211520326717217415324468114467310.0000.0000.000cluster #7 (MAD)7531612295327918714841328705422111329335721095310.0120.0020.537cluster #13 (LA)49099963632107803648325811814632323655010711310.0000.0000.000Total Mean53924476766523257985Table S4: Cortical connectivity. Mean number and standard deviation (SD) of streamlines to 9 different subcortical areas from the five thalamic clusters (C1. C3. C6. C7 and C13) for the males and females group.MALESFEMALESrightleftCluster AN (#6) Video V1: Cluster AN (#6): Axial views of the course of the AN tracks for the males group (left) and the female group (right) in radiological convention. Clusters color code according of the assignment given in Fig. 3b. Tracks are displayed are cluster color for at 50% and in yellow at 100% probability.

Cluster MED (#7) Video V2: Cluster MED (#7): Axial views of the course of the MED tracks for the males group (left) and the female group (right) in radiological convention. Clusters color code according of the assignment given in Fig. 3b. Tracks are displayed are cluster color for at 50% and in yellow at 100% probability.MALESFEMALESrightleft

Video V3: Cluster PO (#1): Axial views of the course of the PO tracks for the males group (left) and the female group (right) in radiological convention. Clusters color code according of the assignment given in Fig. 3b. Tracks are displayed are cluster color for at 50% and in yellow at 100% probability.MALESFEMALESrightleftCluster PO (#1)

MALESFEMALESrightleftCluster LA (#13) Video V4: Cluster #13: Axial views of the course of the LA tracks for the males group (left) and the female group (right) in radiological convention. Clusters color code according of the assignment given in Fig. 3b. Tracks are displayed are cluster color for at 50% and in yellow at 100% probability.

MALESFEMALESrightleftCluster LP (#3) Video V5: Cluster LP (#3): Axial views of the course of the LP tracks for the males group (left) and the female group (right) in radiological convention. Clusters color code according of the assignment given in Fig. 3b. Tracks are displayed are cluster color for at 50% and in yellow at 100% probability.