A Practical Manual of UTILIZATION OF SUPERNATANT OF ONE MICROBE

AS MEDIUM FOR ANOTHER MICROBE

Compiled byG Vijaya Raghavan

ACETOBACTER XYLINUMSupernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommended.

Aspergillus niger Van TieghemSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommended

Aspergillus oryzae (Alberg) CohnSupernatant of B. megaterium autoclaved @ 7.5 ml/L replacing 25% K2HPO4 can be recommended

Azotobacter chroococcum BeijerinckSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommendedSupernatant of B. subtilis autoclaved @ 10 ml/L replacing 50% Nitrogen sources can be recommended

AZOTOBACTER vinelandii LipmanSupernatant of B. megaterium autoclaved @ 10 ml/L replacing 40% K2HPO4and KH2PO4 can be recommended.

BACILLUS BADIUSSupernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommended

BACILLUS CEREUS

Supernatant of L. casei autoclaved @ 25 ml/L replacing 25% Peptone from Casein can be recommended

Bacillus firmusSupernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommended

Bacillus lechiniformis (Weigmann) ChesterSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommended.Supernatant of B. subtilis autoclaved @ 100 ml/L replacing 40% nitrogen sources and 25% Cobalt source can be recommended

Bacillus polymyxa (Prazmowski) MaceSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 can be recommended.

Bacillus pumilus Meyer and GottheilSupernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommended

Bacillus subtilis Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. lichiniformis autoclaved @ 100 ml/L replacing Water can be recommended

Beauveria bassiana (Balsamo) VuilleminSupernatant of citrobacter autoclaved @ 25 ml/L replacing 10% Ammonium Citrate can be recommended

Bifidobacterium brevesupernatant exerts anti-inflammatory effects mediated by dendritic cells (DCs). This supernatant may protect immune system from highly infectious agents such as Salmonella typhi and can down-regulate pro-inflammatory pathways.

LACTOBACILLUS ACIDOPHILUSSupernatant of citrobacter autoclaved @ 25 ml/L replacing 10% Sodium Citrate can be recommended

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% K2HPO4 and KH2PO4 can be recommended.

LACTOBACILLUS ANIMALIS Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% K2HPO4 and KH2PO4 can be recommended.Supernatant of citrobacter autoclaved @ 25 ml/L replacing 10% Sodium Citrate can be recommended

LACTOBACILLUS BULGARICUSSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 and 10% of Cobalt source can be recommended.Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommendedSupernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommended

Lactobacillus casei (Orla-Jensen) Hansen and LesselSupernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommended

Lactobacillus delbrueckii subsp. Delbrueckii (Leichmann) Weiss et al.

Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 can be recommended.Supernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommended

Lactobacillus lactisSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 and 10% of Cobalt source can be recommendedSupernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommendedSupernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommendedSupernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommended

Supernatant of Acetobacter autoclaved @ 50 ml/L replacing Water can be recommendedSupernatant of Citrobacter autoclaved @ 50 ml/L replacing Water can be recommended

Lactobacillus PlantarumSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 and 10% of Cobalt source can be recommendedSupernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommendedSupernatant of S.cereviciae autoclaved @ 250 ml/L replacing 33% Inactivated Dried Yeast and 40% of Yeast Extract can be recommendedSupernatant of Citrobacter autoclaved @ 100 ml/L replacing Water can be recommended

Lactobacillus reuteri

Supernatant of S.cereviciae autoclaved @ 250 ml/L replacing 33% Inactivated Dried Yeast and 40% of Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and KH2PO4 and 10% of Cobalt source can be recommendedSupernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Sodium Acetate can be recommendedSupernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommendedSupernatant of Citrobacter autoclaved @ 100 ml/L replacing Water can be recommended

Lactobacillus rhamnosusSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% K2HPO4 can be recommendedSupernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommended

Lactobacillus sporogenesSupernatant of B. megaterium autoclaved @ 40 ml/L replacing 25% KH2PO4 and Na2HPO4 and 10% of Cobalt Source can be recommendedSupernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Sodium Acetate can be recommendedSupernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommendedSupernatant of S.cereviciae autoclaved @ 250 ml/L replacing 33% Inactivated Dried Yeast and 40% of Yeast Extract can be recommended

Metarhizium anisopliae (Metchnikoff) SorokinSupernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommended

Nitrosomonas Supernatant of B.subtilis autoclaved @75ml/L replacing 10% of Nitrogen source can be recommended.Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 25% KH2PO4 and 10% of Cobalt Source can be recommendedSupernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommendedSupernatant of Nitrobacter @ 200 ml/L replacing water is recommended.

NitrobacterSupernatant of B.subtilis autoclaved @75ml/L replacing 10% of Nitrogen source can be recommended.Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 25% KH2PO4 and 10% of Cobalt Source can be recommendedSupernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommendedSupernatant of Nitrosomonas @ 200 ml/L replacing water is recommended

Paecilomyces fumosoroseusSupernatant of B. megaterium autoclaved @ 50 ml/L replacing 25% KH2PO4 and can be recommendedSupernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommended

Pseudomonas fluorescence MigulaSupernatant of B. megaterium autoclaved @ 60 ml/L replacing 25% Dipotassium phosphate can be recommended

Pseudomonas putidaSupernatant of B. megaterium autoclaved @ 50 ml/L replacing 10% Cobalt source can be recommended

Rhodococcus terraeSupernatant of B. megaterium autoclaved @ 60 ml/L replacing 25% K2HPO4 can be recommendedSupernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommended

Saccharomyces cerevisiae HansenSupernatant contains Ethanol, Inositol, arginase, invertase, cellulase, Alkaline pH, binding of Lactic Acid, efficient conversion of ammonia nitrogen into microbial protein, stimulate the growth of ruminal bacteria, enhance the growth of lactic acid-utilizing bacteria, proteolytic

bacteria and bacteria that convert molecular hydrogen to acetate, enhance the activities of fiber-digesting fungi, tryptophan, prevent colonization by pathogenic microorganisms,

Hence Supernatant is fit to be used to replace the water medium partially in the cases of the following organisms.ACREMONIUM CHRYSOGENUMBifidobacteriumLactobacillus lactisB. subtilis,

In cultivating S cereviciae Supernatant of B. megaterium autoclaved @ 250 ml/L replacing 25%KH2PO4 and K2HPO4 and 10% Cobalt Source can be recommendedSupernatant of S.cereviciae autoclaved @200ml/L replacing 33% of Yeast Extract can be recommendedSupernatant of Citrobacter autoclaved @ 100 ml/L replacing 25%Citric Acid can be recommendedSupernatant of Acetobacter autoclaved @ 100 ml/L replacing Water can be recommended

Streptococcus thermophilusSupernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%natrium glycerophosphate can be recommendedSupernatant of S.cereviciae autoclaved @200ml/L replacing 33% of Yeast Extract can be recommended

Streptomyces avermitilis NBRC No 14893Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%K2HPO4e can be recommendedSupernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Thiobacillus ferroxidans Temple and ColmerSupernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommendedSupernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommended

Thiobacilus thioxidans

Supernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommended

Trichoderma harzianumSupernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommendedSupernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommendedSupernatant of S.cereviciae autoclaved @100ml/L replacing 33% of Yeast Extract can be recommendedSupernatant of Citrobacte autoclaved @100ml/L replacing 20% of Trisodium citrate can be recommended

Trichoderma viridaeSupernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommendedSupernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommendedSupernatant of S.cereviciae autoclaved @100ml/L replacing 33% of Yeast Extract can be recommendedSupernatant of Citrobacte autoclaved @100ml/L replacing 20% of Trisodium citrate can be recommended