Property of BioOps

A Practical Manual of

UTILIZATION OF SUPERNATANT OF ONE MICROBE AS MEDIUM FOR ANOTHER MICROBE

Compiled by

G Vijaya Raghavan

ACETOBACTER XYLINUM

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommended.Aspergillus niger Van Tieghem

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommendedAspergillus oryzae (Alberg) Cohn

Supernatant of B. megaterium autoclaved @ 7.5 ml/L replacing 25% K2HPO4 can be recommendedAzotobacter chroococcum Beijerinck

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommended

Supernatant of B. subtilis autoclaved @ 10 ml/L replacing 50% Nitrogen sources can be recommendedAZOTOBACTER vinelandii Lipman

Supernatant of B. megaterium autoclaved @ 10 ml/L replacing 40% K2HPO4and KH2PO4 can be recommended.

BACILLUS BADIUS

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedBACILLUS CEREUS

Supernatant of L. casei autoclaved @ 25 ml/L replacing 25% Peptone from Casein can be recommendedBacillus firmus

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommended

Bacillus lechiniformis (Weigmann) Chester

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% KH2PO4 can be recommended.

Supernatant of B. subtilis autoclaved @ 100 ml/L replacing 40% nitrogen sources and 25% Cobalt source can be recommended

Bacillus polymyxa (Prazmowski) Mace

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 can be recommended.

Bacillus pumilus Meyer and Gottheil

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedBacillus subtilis

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. lichiniformis autoclaved @ 100 ml/L replacing Water can be recommendedBeauveria bassiana (Balsamo) Vuillemin

Supernatant of citrobacter autoclaved @ 25 ml/L replacing 10% Ammonium Citrate can be recommendedBifidobacterium breve

supernatant exerts anti-inflammatory effects mediated by dendritic cells (DCs). This supernatant may protect immune system from highly infectious agents such asSalmonella typhiand can down-regulate pro-inflammatory pathways.

LACTOBACILLUS ACIDOPHILUS

Supernatant of citrobacter autoclaved @ 25 ml/L replacing 10% Sodium Citrate can be recommended

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% K2HPO4 and KH2PO4 can be recommended.

LACTOBACILLUS ANIMALIS

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% K2HPO4 and KH2PO4 can be recommended.

Supernatant of citrobacter autoclaved @ 25 ml/L replacing 10% Sodium Citrate can be recommended

LACTOBACILLUS BULGARICUS

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 and 10% of Cobalt source can be recommended.

Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommended

Supernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommendedLactobacillus casei (Orla-Jensen) Hansen and Lessel

Supernatant of S.cereviciae autoclaved @ 25 ml/L replacing 50% Yeast Extract can be recommendedLactobacillus delbrueckii subsp. Delbrueckii (Leichmann) Weiss et al.

Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommended

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 can be recommended.

Supernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommendedLactobacillus lactis

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 and 10% of Cobalt source can be recommendedSupernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommended

Supernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommendedSupernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommended

Supernatant of Acetobacter autoclaved @ 50 ml/L replacing Water can be recommended

Supernatant of Citrobacter autoclaved @ 50 ml/L replacing Water can be recommended

Lactobacillus Plantarum

Supernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and NaH2PO4 and 10% of Cobalt source can be recommended

Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Potassium Acetate can be recommended

Supernatant of S.cereviciae autoclaved @ 250 ml/L replacing 33% Inactivated Dried Yeast and 40% of Yeast Extract can be recommendedSupernatant of Citrobacter autoclaved @ 100 ml/L replacing Water can be recommended

Lactobacillus reuteriSupernatant of S.cereviciae autoclaved @ 250 ml/L replacing 33% Inactivated Dried Yeast and 40% of Yeast Extract can be recommendedSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% Na2HPO4 and KH2PO4 and 10% of Cobalt source can be recommended

Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Sodium Acetate can be recommended

Supernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommended

Supernatant of Citrobacter autoclaved @ 100 ml/L replacing Water can be recommended

Lactobacillus rhamnosusSupernatant of B. megaterium autoclaved @ 5 ml/L replacing 25% K2HPO4 can be recommended

Supernatant of S.cereviciae autoclaved @ 100 ml/L replacing 33% Inactivated Dried Yeast can be recommendedLactobacillus sporogenes

Supernatant of B. megaterium autoclaved @ 40 ml/L replacing 25% KH2PO4 and Na2HPO4 and 10% of Cobalt Source can be recommended

Supernatant of Acetobacter autoclaved @ 25 ml/L replacing 10% Sodium Acetate can be recommended

Supernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommended

Supernatant of S.cereviciae autoclaved @ 250 ml/L replacing 33% Inactivated Dried Yeast and 40% of Yeast Extract can be recommendedMetarhizium anisopliae (Metchnikoff) Sorokin

Supernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommended

Nitrosomonas

Supernatant of B.subtilis autoclaved @75ml/L replacing 10% of Nitrogen source can be recommended.

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 25% KH2PO4 and 10% of Cobalt Source can be recommended

Supernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommended

Supernatant of Nitrobacter @ 200 ml/L replacing water is recommended.

Nitrobacter

Supernatant of B.subtilis autoclaved @75ml/L replacing 10% of Nitrogen source can be recommended.

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 25% KH2PO4 and 10% of Cobalt Source can be recommended

Supernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommended

Supernatant of Nitrosomonas @ 200 ml/L replacing water is recommendedPaecilomyces fumosoroseusSupernatant of B. megaterium autoclaved @ 50 ml/L replacing 25% KH2PO4 and can be recommended

Supernatant of L Casei autoclaved @ 100 ml/L replacing 10% Cassein can be recommended

Pseudomonas fluorescence Migula

Supernatant of B. megaterium autoclaved @ 60 ml/L replacing 25% Dipotassium phosphate can be recommended

Pseudomonas putida

Supernatant of B. megaterium autoclaved @ 50 ml/L replacing 10% Cobalt source can be recommended

Rhodococcus terrae

Supernatant of B. megaterium autoclaved @ 60 ml/L replacing 25% K2HPO4 can be recommended

Supernatant of S.cereviciae autoclaved @150ml/L replacing 40% of Yeast Extract can be recommended

Saccharomyces cerevisiae Hansen

Supernatant contains

Ethanol, Inositol, arginase, invertase, cellulase, Alkaline pH, binding of Lactic Acid, efficient conversion of ammonia nitrogen into microbial protein, stimulate the growth of ruminal bacteria, enhance the growth of lactic acid-utilizing bacteria, proteolytic bacteria and bacteria that convert molecular hydrogen to acetate, enhance the activities of fiber-digesting fungi, tryptophan, prevent colonization by pathogenic microorganisms, Hence Supernatant is fit to be used to replace the water medium partially in the cases of the following organisms.

ACREMONIUM CHRYSOGENUM

Bifidobacterium

Lactobacillus lactis

B. subtilis,

In cultivating S cereviciae

Supernatant of B. megaterium autoclaved @ 250 ml/L replacing 25%KH2PO4 and K2HPO4 and 10% Cobalt Source can be recommended

Supernatant of S.cereviciae autoclaved @200ml/L replacing 33% of Yeast Extract can be recommended

Supernatant of Citrobacter autoclaved @ 100 ml/L replacing 25%Citric Acid can be recommended

Supernatant of Acetobacter autoclaved @ 100 ml/L replacing Water can be recommended

Streptococcus thermophilus

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%natrium glycerophosphate can be recommended

Supernatant of S.cereviciae autoclaved @200ml/L replacing 33% of Yeast Extract can be recommended

Streptomyces avermitilis NBRC No 14893

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%K2HPO4e can be recommended

Supernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Thiobacillus ferroxidans Temple and Colmer

Supernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommended

Thiobacilus thioxidans

Supernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommended

Trichoderma harzianum

Supernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommended

Supernatant of S.cereviciae autoclaved @100ml/L replacing 33% of Yeast Extract can be recommended

Supernatant of Citrobacte autoclaved @100ml/L replacing 20% of Trisodium citrate can be recommended

Trichoderma viridae

Supernatant of B.subtilis autoclaved @200ml/L replacing 33% of Nitrogen sourcet can be recommended

Supernatant of B. megaterium autoclaved @ 100 ml/L replacing 10%KH2PO4e can be recommended

Supernatant of S.cereviciae autoclaved @100ml/L replacing 33% of Yeast Extract can be recommended

Supernatant of Citrobacte autoclaved @100ml/L replacing 20% of Trisodium citrate can be recommended