Molecular Constellations 2014Optics beyond the Abbe Limit

C. Cremer

Institute of Molecular Biology (IMB), Mainz/DEInstitute for Pharmacy & Molecular Biotechnology (IPMB)/Kirchhoff-Institute for

Physics (KIP), University Heidelberg

For more information see www.imb-mainz.de

Letiz

ia M

anci

no

Heidelberg Mainz

Molecular Constellations in Mouse PachyteneChromosomes

K. Prakash, M. Borsos, S. Redl, H.-K. Lee, K. Tachibana-Konwalski, R. Ketting, C.Cremer, U. Birk

Conventional Microscopy Localization Microscopy (SPDM)

500 nm

Conventional Structured Illumination Localization microscopy

Structured illumination microscopy in human retinadiagnostics:profiles of particles smaller than 500 nm

S. R

ossb

erge

ret a

l. (2

013)

Br.

J. O

phta

lmol

. 97

(4):

518

–52

3.

Abstract. We survey the history of resolution enhancement techniquesin microscopy and their impact on current research in biomedicine…we present the historical development of various techniques thatsubstantially enhance the optical resolution of the light microscope…

pp 281–344.

3 point-like objects in x,y plane withnext neigbour distances 50 nm

a) Labelling with same spectralsignature

b) Labeling with different uniquespectral signatures B,G,R

Computation (scalar Theory):

NA = 1.4, λexc = 488 nm

Optical Isolation by the

“Supernova“ Mode: Induction of

stochastically distributed short

flashes of light emission by

individual moleculesCremer et al. 1996, 1999, 2010, 2011

Principle of Spectrally Assigned Localization Microscopy (SPDM): Optical Isolation of Diffraction Images of individual Molecules: Determination of Gravity Centers

Publications Cremer Lab 2013

G. Best, R. Amberger, C. Cremer (2013) Super-Resolution Microscopy:Interference and Pattern Techniques. In: Fluorescence Microscopy: FromPrinciples to Biological Applications (Kubitscheck U,Ed.), pp. 345 – 374. Wiley-Blackwell.

C. Cremer, B.R. Masters (2013) Resolution enhancement techniques inmicroscopy. Eur. Phys. J. H, Eur. Phys. J. H 38: 281–344.

S. Rossberger, T. Ach, G. Best, C. Cremer, R. Heintzmann, S. Dithmar (2013)High resolution imaging of autofluorescent particles within drusen usingstructured illumination microscopy, British Journal of Ophthalmology 97: 518 –523.

S. Rossberger, G. Best, D. Baddeley, R. Heintzmann, U. Birk, S. Dithmar, C.Cremer (2013) Combination of structured illumination and single moleculelocalization microscopy in one setup, J. Opt. 15, doi:10.1088/2040-8978/15/9/094003.