summer school in ncsr demokritos summer 2005
DESCRIPTION
Summer School in NCSR Demokritos Summer 2005 Methods in micro – nano technology and nanobiotechnology Organizer: National Center for Scientific Research “Demokritos” Participating Institutes: Microelectronics, Radioisotopes and Radiodiagnostic Products, Physical Chemistry - PowerPoint PPT PresentationTRANSCRIPT
Summer School in NCSR DemokritosSummer 2005
Methods in micro – nano technology and nanobiotechnology
Organizer: National Center for Scientific Research “Demokritos”
Participating Institutes: Microelectronics,
Radioisotopes and Radiodiagnostic Products, Physical Chemistry
Information: www.imel.demokritos.gr, Contact: Evangelos Gogolides, [email protected]
Rational:
Modern Research takes advantage of Micro and Nanotechnology developments.
Merging areas of research (Nanobiotechnology) demand interdisciplinary skills.
Necessary for researchers from Life Sciences, Chemistry, and Engineering to acquire skills in Micro and Nanotechnologies.
Target: Establish common language between the various disciplines-promote interdisciplinary research
Content: Five (5) day intensive summer school Offers: classroom and laboratory experience on:
micro and nano-technology processes / materials / applicationsTargeted in: Nanobiotechnology
Who should attend:
Group leaders involved in molecular biology or biotechnology,Post Doctoral FellowsGraduate students with Engineering, Science or Life Science background, All those who wish to apply micro-technology in their research.
Maximum number of registrants 20 persons. Fees: 1000 Euro (accommodation NOT included)Dates: 6-10 June 2005.
Synergies with other schools in Greece and Nano2life: Back to back with Practical Course on Advanced techniques in molecular biologyorganized by FORTH/ICEHT, Date: 13-18 June 2005http://mb_school.iceht.forth.gr A small reduction in the fee for those taking both seminars is being considered.
Syllabus 1:
Lecture 1: Introduction to micro and nano-technology and nanobiotechnology
Lecture 2: Patterning technologies (lithography and plasma etching) for Si, glass, and plastic substrates in the fabrication of analytical/bioanalytical microdevices
Laboratory 1: Fabrication of microfluidic devices on plastic substrates by novel lithographic techniques.
Syllabus 2:
Laboratory 2: Fabrication of plastic Capillary Electrophoresis Devices by Lithography and plasma etching techniques.
Lecture 3: Principles of Integrated Biosensing Devices
Laboratory 3: Operation of a lab-on-a-chip optical device using model assays and real time measurements
Monolithic silicon optocouplers
PMMA Capillaries
Syllabus 3:
Lecture 4: Patterning of biomolecules and other biological substances
Laboratory 4a: Part A: Fabrication of protein microarrays using biocompatible photoresist-based lithography.
Laboratory 4b: Part B. Fluorescence detection of protein arrays.
Fluorescence picture of the rabbit γ-globulins and biotinylated-BSA spot arrays after a 2 h immunoreaction with a mixture of AF 546 labeled streptavidin (red spots) and AF 488 labeled anti-rabbit IgG antibody (green spots). The spot size is approximately 4 μm.
Twelve rows of different protein spots fabricated in 12 succesive lithographic steps
Syllabus 4:
Lecture 5: Biomolecules as building blocks of molecular electronics
Laboratory 5: Nano-patterning by electron beam lithography
Lecture 6: Protein and DNA arrays: fabrication, detection and applications.
Laboratory 6: Demonstrating a capillary fluoroimmunosensor
Syllabus 5:
Lecture 7: Drug Delivery, Liposomes, Dendrimers, Cyclodextrins
Laboratory 7: Dynamic light scattering and Z-potential measurements, Video enhanced optical microscopy of Liposomes and Atomic Force Microscopy
Atomic Force Microscopy
Formation of DNA nanoparticles of ~40 nm diameter
PC-CHOL-ODPG / DHP Liposomes
10 100 1000 10000 100000
r (nm)
55μμmm
PEG 0%PEG 5%PEG 15%
55μμmm
55μμmm
55μμmm
55μμmm
55μμmm
55μμmm
Liposome-liposome interactionsCorrelation of Optical Microscopy and Dynamic Light Scattering results
Laboratory 7: Dynamic light scattering optical microscopy
Syllabus 6:
Laboratory 8: Drug inclusion monitoring in situ by NMR spectroscopy X-ray diffraction characterisation of drug inclusion in Cyclodextrins and 3-D visualisation
(OH)n
NH26
ppm (t1) 3.504.004.505.00
Syllabus 7: