sukrasno siti kusmardiyani
TRANSCRIPT
SUKRASNO SITI KUSMARDIYANI
Physical and Chemical Parameters of Crude Drugs and Their Products
PART 1
Geneva, 9-13 October 1978 A “ Meeting on selection and characterization of medicinal plants (vegetable drugs)”
Suggested that “In view of the current importance ofand interest in vegetable drugs, it is necessary to preparean international Codex containing general control methodsand specific monograph on such drugs”.
1. Microscopical and histochemical techniques for the morphological analysis of the drugs, whole or in powder form
2. Techniques for determination of loss on drying and water content
3. Techniques for the determination of extractable substances, essential oils or ash
4. Tests to determine the presence of undesirable substances (adulterants, pesticides, preservatives, etc.)
5. Any other general test required, particularly the test for purity (freedom from microorganisms and parasites)
General Control Methods
1. USP United States Pharmacopoeia XIX, 19752. BP British Pharmacopoeia 19733. EuP European Pharmacopoeia I-1969, II-1971, III-1975,
Supplement to Volume II-1973, to Volume III- 19774. SSRP State Pharmacopoeia of the Union of Soviet Socialist Republics, X,
1968 (in Russian)5. ChP Pharmacopoeia of the People’s Republic of China, 1978 (in Chinese)6. JP The Pharmacopoeia of Japan, IX (English edition), 19767. IndP Pharmacopoeia of India, I, 19558. IP The International Pharmacopoeia III, I (1951, 55, 59), II (1967)9. PhFr. Pharmacopée Française, IX, 1972
10. PhHelv. Pharmacopoeia Helvetica, VI, 197111. PhIt. Farmacopea Ufficiale della Repubblica Italiana, VIII, 197212. PhDDR Arzneibuch der Deutschen Demokratischen Republik, II, 197513. Ph Hung. Pharmacopoea Hungarica, VI (English edition), 1970
The Pharmacopoeias Consulted
Lou, Z., 1980, General Control Method for Vegetable Drugs, Comparative Study of Methods Included in Thirteen Pharmacopoeias and Proposals on Their International Unification.
1. General notices2. Powder fineness and sieve size3. General advise on sampling4. Determination of foreign matter5. Macroscopic and microscopic examination6. Thin–layer chromatography7. Determination of ash8. Determination of extractable matter9. Determination of water and volatile matter
10. Determination of volatile oils
Chapters to be discussed:
Topics 5, 7, 8, 9, and 10 will be more elaborated as part of laboratory activities.
“dry to constant weight”
“precision”
- General notices -
“solubility”
Describe these commonly used terms:
WHO, 1998, Quality Control Methods for Medicinal Plant Materials.
“storage condition”
In a refrigerator
Cold or cool
Room temperature
… – … oC
…. – … oC
… – … oC
- General notices -
Find other terms and explain.
- Microscopic examination -
Clarification of microscopic particles
Chloral hydrate
Calcium carbonate
Calcium oxalate
- Microscopic examination -
Andrographidis herba
Cannabis herba
© SK
© SK
Ver. 22SK005
+ HCl
+ HCl
Explain
©KUSMARDIYANI
ERGASTIC CELL CONTENT
Trease, 537.
Calcium oxalate crystals
A-D tetragonal systemE-I monoclinic system
A1-A3 Rosette crystals of tetragonal system
D a tetragonal prismE a monoclinic prismG raphidesH a single needle crystalI a sphaerocrystal
- Microscopic examination -
Rheum officinale Baillon.Polygonaceae
RHEI RADIX
Source: root and rhizome of Rheum officinale Baillon, R. palmatum L., other species of Rheum except R. rhaponticum L.
Rhubarb, Rheum, Chinese rhubarb, Kelembak
Rein-8-glukosida
Tyler, 64
© KUSMARDIYANI
AKAR KELEMBAK
Ver. 22SK005
MMI IV, 231-233.
SERBUK AKAR KELEMBAK
PENAMPANG MELINTANG AKAR KELEMBAK
RHEI RADIX
Stahl, 89.
RHEI RADIX
Rhei radix© KUSMARDIYANI
Ver. 22SK005
Write microscopic information of this pulverized crude drug.
Alstonia scholaris R. Br. Apocynaceae
ALSTONIAE CORTEX
© SK
Ver.22SK005
KULIT BATANG PULE
© SK
Alstonia scholaris R. Br.Apocynaceae
© KUSMARDIYANI
MMI IV, 3 - 5.
ALSTONIAE CORTEX
ALSTONIAE CORTEX
Tombol pengatur halus mikroskop diputar sehingga dapat terfokuskan pada daerah bawah medan pengamatan.
Kristal kalsium oksalat yang sebagian berbentukmonoklinik, karena pembiasan cahaya dan penggeseranfokus pengamatan pada saat tombol halus diputar, akanterlihat seperti “buku atau jendela terbuka”.
Ver. 22SK005
“flour and starch”
Explain the differences:
How much should be collected from each container ?
• Crude drug has specific characters, i.e.: low homogeneity, and need specific sampling method.
Sampling of Crude Drug
Sample Collection
Microscopic Particle Size
• Microscope• Ocular Micrometer• Objective Micrometer
Equipments:
Steps of Measurement
1. Calibrate ocular micrometer with objective micrometer2. Measurement of particle
Calibration:• Ex. :100 scales of ocular superimposed with 30 scale
of objective micrometer. • 100 scale = 30 x 0,01 mm• 1 scale = 3 µm
WHO, 1998, Quality Control Methods for Medicinal Plant Materials.
Alignment of the stage micrometer and the ocular micrometer
Definition of foreign matter• Parts of medicinal plant material or material other
than those named with limits scpecified for the plant material concerned.
• Any organism, part of an organism, other than that named in the specification and description of the plant material concerned.
• Mineral admixtures not adhering to the medicinal plants materials, such as soil, stones, sand, and dust.
Determination of Foreign Matter
Sample size• Roots, rhizomes, and barks 500 g• Leaves, flowers, seeds, and fruits 250 g• Cut medicinal plants
with average weight of fragment < 0.5 g 50 g
Recommended procedures• Spread the sample and collect foreign matter
• Sift the remainder through sieve no 250, and the dust mineral admixture.
• For foreign matter similar to the crude drug, critical test that base on physical and chemical tests may be needed.
Water Content and Lost on Drying
• Water content is the water present in the crude drug.
• Lost on drying is the decrease of crude drug weight on drying at 105oC.
• Compounds evaporate at 105oC is not only water.
• Lost on drying is the rapid method to estimate water content.
Water Content Determination
Azeotrop DistillationWater forms azeotrop mixture with toluene or xylene, evaporate together and separate following condensation. The distillate is collected in scaled tube.
The Saturation of Toluene• Toulene is mixed with water and distilled until the
volume of water in the distillate remain constant.
• Toluene is shaken with water and the excess of water separated.
EuPPhDDR
USP & ChP
SSRP
Apparatus for the determination of water by azeotropic distillation
Differences:-Capacity of distilling flask-Length of condenser-Length of vertical portion of connecting tube-Receiving tube-Distilling liquid-Quantity of sample taken-Anti-bumping material-Distillation rate
Lou, Z., 1980, General Control Method for Vegetable Drugs, Comparative Study of Methods Included in Thirteen Pharmacopoeias and Proposals on Their International Unification.
Apparatus for the determination of water content by azeotropic method
WHO, 1998, Quality Control Methods for Medicinal Plant Materials
dimensions in mm
Learn more about the method!
Apparatus to determine water content by distillation methodinMATERIA MEDIKA INDONESIA
Compare with method and apparatus recommended by the WHO
KARL FISHER TITRATION
• Not suitable for crude drug• Suitable for extract
Explain!!
BP
PhDDR
SSRP Method 2
SSRP Method 1
Apparatus for the determination of volatile oils in vegetable drugs
Differences:- Weight of sample- Distillation liquid- Capacity of flask - Rate of distillation- Volume of xylene added- Duration of distillation- Capacity & subdivision of
measuring tube
Lou, Z., 1980, General Control Method for Vegetable Drugs, Comparative Study of Methods Included in Thirteen Pharmacopoeias and Proposals on Their International Unification.
Differences:- Weight of sample- Distillation liquid- Capacity of flask - Rate of distillation- Volume of xylene added- Duration of distillation- Capacity & subdivision of
measuring tube
Lou, Z., 1980, General Control Method for Vegetable Drugs, Comparative Study of Methods Included in Thirteen Pharmacopoeias and Proposals on Their International Unification.
JP PhHelv in mm
ChP in cm
Apparatus for the determination of volatile oils in vegetable drugs
Differences:- Weight of sample- Distillation liquid- Capacity of flask - Rate of distillation- Volume of xylene added- Duration of distillation- Capacity & subdivision of
measuring tube
Lou, Z., 1980, General Control Method for Vegetable Drugs, Comparative Study of Methods Included in Thirteen Pharmacopoeias and Proposals on Their International Unification.
EuP Apparatus for the determination of volatile oils in vegetable drugs
Apparatus to determine volatile oils
WHO, 1998, Quality Control Methods for Medicinal Plant Materials.
dimensions in mm
Apparatus to determine volatile oil contentinMATERIA MEDIKA INDONESIA
Compare with the method and apparatus recommended by the WHO
Macroscopic and Microscopic Examination
Macroscopic inspection:shape, size, colour, surface characteristic, texture, fracture characteristic, appearance of the cut surface
Microscopic inspection:preparation of specimen, treatment using chemical reagents,characteristic fragments,types of stomata & stomatal index,etc.
Take appropriate notes from this textbook.
How many crude drugs in this mixture?
© KUSMARDIYANI
Macroscopic Examination
Use forceps to separate each component of the mixture. There are 12 crude drugs. Now write common name for each of these crude drugs!
The answers can be seen on the next slide.
© KUSMARDIYANI
Now write Latin name for each of these crude drugs.
If you don’t know the right answers, go the next slide.
Bunga kenanga
Bunga cengkeh
Daun sirih
Rimpang laos
Rimpang kunir
Daun kemuning
Kulit batang lemo
Buah ketumbar
Buah pinang
Biji kopi
Daun jambu biji
Daun jati belanda
© KUSMARDIYANI
Canangii flos
Caryophylli flos
Piperis betle folium
Languatis rhizoma
Curcumae domesticae rhizomae
Murrayae folium
Litseae cortex
Coriandri fructus
Arecae semen
Coffeae semen
Psidii folium
Guazumae folium
© KUSMARDIYANI
Try once again to mention Latin name for each of these medicinal plant materials.
1
2
3
4
5
6 7
8
9
10
11
© KUSMARDIYANI
It seems that you can only get 11 items unless you make them separated before identifying each of these crude drugs
Languatis rhizoma
© KUSMARDIYANI
If you still have difficulties, try to start again from the first slide.
Canangii flos
Piperis albi fructus
Psidii folium
Guazumae folium
Curcumae domesticae rhizoma Murrayae folium
Piperis betle folium
Arecae semen
Coffeae semen
Litseae cortex
MICROSCOPIC EXAMINATION A mixture of 4 powdered crude drugs
© KUSMARDIYANI
Ver. 22SK005The mixture was observed using chloral hydrate solution
Arecae semenArtemisiae herba Curcumae domesticae
© KUSMARDIYANI
Ver. 22SK005
This powdered crude drug was observed using chloral hydrate solution
How to identify the starch? Another preparation using water (+ glycerin) is needed. -- Explain --
Amylum solaniCurcumae domesticae rhizoma
© KUSMARDIYANI
Ver. 22SK005
The same mixture was observed using water (+ glycerin)
How do you identify that these starches come from different sources? Explain.
Write Latin name of the 4 crude drugs in this mixture. The answers are on the next slide.
© KUSMARDIYANI
Ver. 22SK005
Powdered crude drug was observed using chloral hydrate solution
Sappan lignumPsidii folium
Parkiae semen
Theae foliumTry to obtain the characteristic fragments
by moving to other parts of the slide.
© KUSMARDIYANI
Ver. 22SK005
Give complete descriptions of these characteristic fragments.
LABORATORY OF PHARMACOGNOSY - SCHOOL OF PHARMACY - INSTITUT TEKNOLOGI BANDUNG
More examples of crude drug examination can be learned from:
- Kusmardiyani, School of Pharmacy ITB -
1. General notices2. Powder fineness and sieve size3. General advise on sampling4. Determination of foreign matter5. Macroscopic and microscopic examination6. Thin–layer chromatography7. Determination of ash8. Determination of extractable matter9. Determination of water and volatile matter
10. Determination of volatile oils
We have discussed several basic concepts and significant points of their application. Do the best you can to summarize each chapter below and explain each step of the recommended procedures.
WHO, 1998 (2005), Quality Control Methods for Medicinal Plant Materials.
------Check your notes soon after class and briefly review the notes before the next class-----