suicide ideation in the depression group (nbb)
DESCRIPTION
Anterior Cingulate Cortex. Suicide Ideation in the depression group (NBB). *. **. *. A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts. - PowerPoint PPT PresentationTRANSCRIPT
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Suicide Ideation in the depression group (NBB)A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts
GABRD
NoSuicideIdeation SuicideIdeation0.00
0.05
0.10
0.15
0.20
0.25
GABBR2
NoSuicideIdeation SuicideIdeation0.8
1.0
1.2
1.4
1.6
GluR1
NoSuicideIdeation SuicideIdeation0.14
0.16
0.18
0.20
0.22
0.24* * **
DLPFC was also checked, however, none of the target genes were unchanged.
Anterior Cingulate Cortex
Juan Zhao and Dick Swaab, submitted
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Microglia isolation procedure
• Mechanic & enzymatic dissociation
• Cell separation: Percoll gradient
• Take off myelin
• Collect cells from interface
• Stain cells with labeled antibodies:o CD11b - PEo CD45 - FITCo CD15 - APC
• Selection using magnetic CD11b beads
Melief et al. Glia 2012
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Isolation and phenotyping of pure primary human microglia
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Peripheral inflammation impacts on microglia activation
Melief et al. Glia 2012
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The Netherlands Brain Bank for Psychiatry
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Phenotype & Characterize human post mortem microglia
Jeroen Melief, thesis 2014
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