studies on cycloferon by victoria, ruben gombalandi, immunomodulators
DESCRIPTION
Effect of cycloferone on the gastric mucosa of ratsTRANSCRIPT
![Page 1: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/1.jpg)
CYCLOFERON INDUCED CHANGES IN STOMACH OF OLD RATS
REPORT PRESENTED BY RUBEN GOMBALANDI,HEADED BY MISS VICTORIA
MINISTRY OF HEALTH UKRIANE LUGANSK STATE MEDICAL UNIVERSITYDEPARTMENT OF BIOLOGICAL SCIENCE
![Page 2: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/2.jpg)
INTRODUCTION
• BOTH Interferon and inductor of endogenous production of interferon have gained clinical acceptance in recent years. WHY?
• They have antiviral and immunomodulatory property• They have no side effect• They can be use for long term therapy.
![Page 3: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/3.jpg)
immunomodulators
IMMUNE MODIFIERS
Immunosuppresants
Immune tolerance
Immunostimulants
![Page 4: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/4.jpg)
LevamisoleThalidomideBCGRecombinant Cytokines
InterferonsCyclopheron*
IMMUSTIMULATORS
![Page 5: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/5.jpg)
CyclopheronAntiviralImmunomodulatory activityBind to cell surface receptors – initiate intracellular eventsEnzyme inductionInhibition of cell proliferationEnhancement of immune activitiesIncreased Phagocytosis
Uses : HSV infection; influenza and acute respiratory viral diseases; Chronic viral hepatitis B and C; Acute intestinal infections; secondary immunodeficiency associated with chronic bacterial and fungal infections.
![Page 6: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/6.jpg)
Scheme of experiment:Age Group kind of
influenceDuration of the experiment in days and the number of animals
old rats (mass of 330 - 350 gr.)
7 day 15 days
30 days
90 days
180 days
1 Cyclopheron
6 6 6 6 6
2 Control of cyclopheron
6 6 6 6 6
Total 60
![Page 7: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/7.jpg)
What Was use for the study
LIGHT MICROSCOPY and Morphometric methood was used for the study.
![Page 8: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/8.jpg)
FINDINGSCONTROL group• Normal• Normal
EXPERIMENTAL GROUP• Rats killed 7 days after
injection,number of chief cell droped by 13% compared to the Contro group.
• Area of zymogenic cells and their nucleus was less by 12% and 15% compare to the control group
![Page 9: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/9.jpg)
Common Findings
IN BOTH GROUPS THE AVERAGE NUMBER OF PARIETAL CELLS AND THE AREA OF THEIR NUCLEUS WAS THE SAME.
![Page 10: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/10.jpg)
RESULT OF CYCLOFERONE ON OLD RATS AFTER 7 DAYS
![Page 11: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/11.jpg)
7дн. 15дн. 30дн. 90дн. 180дн.0
10
20
30
40
50
60
*
**
*
*
*
*
ГК,ЦФ ГК,КЦФ ПК,ЦФ ПК,КЦФ
![Page 12: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/12.jpg)
DAY 15AND 30
At Day 15, and 30 changes in the number and morphometric parameters of the chief and parietal cells were not revealed.
![Page 13: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/13.jpg)
Result on the 15th Day
![Page 14: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/14.jpg)
BY DAY 90
By the 90th Day, Parietal cells showed an increase in number by 42%, but morphometric data did not show any increase in functional activity.
![Page 15: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/15.jpg)
At the End of the experiment
Parietal cells inceased by 21% and the chief cells increased by 28%.But there was not difference in data of area and diameter of cell between experimental and control group.
![Page 16: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/16.jpg)
SYNOPSIS LEADING TO OUR CONCLUSION
Ageing leads to decrease response of the cells to cytokines.,also gene expression of the IL-2 descrease with ageing of the immune system. But exogenous IL-2 restores the ability of CD4+ to respond by proliferation through the Transforming growth factor. TGF alpha,play a role in the regulation of acid secretion and mucosal renewal in the stomach of old rats.therefore we suggest t
![Page 17: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/17.jpg)
IN CONCLUSION
We suggest that cycloferon activate T- cells (CD4 + lymphocytes) that produce IL- 2 which via the TGF-α expressed by stomach chief and parietal cells mediate proliferation of secretory cells to the end of experiment.
![Page 18: Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS](https://reader036.vdocuments.us/reader036/viewer/2022062405/55843178d8b42a79568b537e/html5/thumbnails/18.jpg)