Strawberry DNA Strawberry DNA ExtractionExtraction

DNA: BasicsDNA: BasicsDeoxyribonucleic acid Genetic material

◦ Chain of molecules linked together◦ DNA contains instructions for traits

Wrapped tightly around “histones”

Forms chromosomesHoused in nucleus

DNA: BasicsDNA: Basics

DNA: StructureDNA: StructureDouble-helix (twisted ladder)Sugar and phosphate backboneNitrogen basesSubunit: “nucleotide”

DNA: ReplicationDNA: ReplicationSpecial pairing between nitrogen

basesNew strand is copy of old strand

DNA Extraction: Step 1DNA Extraction: Step 1Break the cells open (using force)

DNA Extraction: Step 2DNA Extraction: Step 2Dissolve the cell membrane and

nuclear envelope (using detergent/soap)

Current Protocols in Protein SciencePublished by John Wiley & Sons, Inc.

DNA Extraction: Step 3DNA Extraction: Step 3Remove proteins that are bound

to DNA (using salt to dissolve them)

DNA Extraction: Step 4DNA Extraction: Step 4Precipitate DNA (with ethanol)

DNA Extraction: Why?DNA Extraction: Why?Species identification

VS.

DNA Extraction: Flow DNA Extraction: Flow chartchart

Bag already has extraction solution.

Secure cheesecloth to beaker.

Do NOT disturb tube!

Collect DNA.

Gently pour 20 mL ethanol into tube.

Transfer liquid to test tube.

Filter liquid through cheesecloth into beaker.

Mix by rocking bag slowly.

Place bag on ice for 1 minute.

Crush fruit for 5 minutes.

Squeeze out air and close bag.

Add fruit to zip-lock bag.

DNA Extraction: Flow DNA Extraction: Flow chartchart

Bag already has _______________ solution.

Secure ________________ to beaker.

Do NOT disturb tube!

Collect _______!

Gently pour ____ mL ethanol into tube.

Transfer liquid to ______ ______.

Filter liquid through cheesecloth into beaker.

Mix by rocking bag slowly.

Place bag on _______ for 1 minute.

Crush fruit for ____ minutes.

Squeeze out air and close bag.

Add fruit to zip-lock bag.