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Lecture 5 Slides

Go to TA sections; challenge them!

Come to R’s and/or TAs office hours2130 Pac Hall Mon 2-3

Tue 2-3

details on course web site:

http://course.ucsd.edu/rhampton/bibc102

Please

Please

READ Glucose, Glycolysis and Krebs

But First

Please

READ First Half of The NAME GAME

READ Glucose, Glycolysis and Krebs

hppt://courses.ucsd.edu/rhampton/bibc102

fig 14-1

Things to do with glucose…

adenosine triphosphate… ATP

fig 13-11

free energies are additive!

11kg

68kg

riddle: how do you lift 11 kg? ans: lower a weight > 11kg!

In metabolism,it is all about thecoupling mechanism

fig 13-9

fig 14-2

It takesATP to make ATP!

the preparatory phase

fig 14-2

delayedgratificationin glycolyticcatabolism

the payoff phase

fig 14-3

Glucose catabolismaround the bios…

pg 532

Phosphorylation of glucose

Hexokinse: an example of induced fit

fig 6-22

Hexokinse: an example of induced fit

fig 6-22

pg 532

Isomerization of glucose 6-P

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Anomers…

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The structural simplicity of isomerase reaction

pg 532

Phosphorylation of fructose 6-P

pg 533

Cleavage of fructose 1,6 bisphosphate by aldolase

fig 14-6

Cleavage of fructose 1,6 bisphosphate by aldolase

now have two distinct3-carbonmolecules

pg 534

DHAP isomerization gives us 2 identical G3P

same chemistry, different enzyme

Isomerase action is familiar…

Equivalent carbons from different parts of glucose

fig 14-6

pg 535

A fancy redox reaction…

fig 14-7

A fancy redox reaction catalyzed by GAPDH

fig 14-7

The GAPDH active site

Enyzme-substrate complex

fig 14-7

fig 14-7

a covalentintermediatebetween acysteine Sand the substrate

The carbonmoleculegets oxidized!!

fig 14-7

fig 14-7

Phosphate as a nucleophile!!??

fig 14-7

releaseof theproduct,1,3 BPG

fig 14-7

Ready for more substrate…

fig 14-7

This is the product of interest!

pg 537

Voila! Our first ATP!

XX

Substrate level phosphorylation: the general idea

pg 537

“Isomerization” of 3PG to make 2PG

“mutase” is isomerase that moves groups

fig 14-8 (fourth)

A low level intermediate must be produced

pg 538

Making an enol form by removal of H2O

pg 538

A second substrate level phosphorylation: more ATP!

pg 538

Ta Da!!! Pyruvic acid

pg 547

LDH converts pyruvate to lactate, restoring the pool of NAD+

Glycolysis: energy without O2

anaerobic organisms

anaerobic situations

neutrophil

CEOLACANTH: the Living Fossil

Glycolysis in action: 100 mt sprint

Usain Bolt!!