starch digestion by amylase - austin peay state university · ppt file · web view2017-07-11 ·...
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Lab 8
Digestion
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Lab 8: Digestion• Histology
– Parotid gland, esophagus, stomach, duodenum, jejunum, ileum, colon, liver, pancreas
– Alimentary canal histological layers
• Models of the digestive system
• PhysioEx: Chemical and Physical Processes of Digestion– Learn to understand and interpret data tables + simulated test tube
results for salivary amylase
– Learn about the major digestive enzymes and their substrates
– Examine the effect of heating, cooling, and pH on enzyme activity
– Use indicators of enzyme activity (IKI, Benedict’s, etc.)
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General Structure• Digestive organs
divided into 2 main groups – GI (alimentary)
tract – Accessory
structures • cheeks, teeth,
tongue, salivary glands
• liver, gallbladder, pancreas
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Teeth
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General Organization of GI Tract
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Histology of the GI Tract#55: salivary glands#56: esophagus#57: stomach#58: small intestine – duodenum#59: small intestine - jejunum#60: small intestine – ileum#54: liver#39: pancreas#61: large intestine
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Salivary Glands
MA = mucous acinus; SA = serous acinus; ducts lined by simple cuboidal epithelium
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Parotid Salivary Gland
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Esophagus
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Esophagus
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Esophagus – 4 tissue layers
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Esophagus
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Esophagus
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Transition Zone
Stratified squamous
Simple columnar
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Stomach
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The interior wall of the stomach consists of a series of folds, called rugae.
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Detail of Rugae
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Fundus:gastric glands
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Gastric Glands
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G Cells produce Gastrin
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Duodenum
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Duodenum
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Villi (multicellular)
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Duodenum
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Villi and Intestinal Crypts
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Duodenum
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Jejunum
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Jejunum
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Ileum with MALT = Mucosal Associated Lymphatic Tissue
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Ileum
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Ileum with Peyer’s Patches = aggregated lymphatic nodules
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Ileum and Plicae Circularies
Lacteal
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Liver in cross section
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Liver Lobules
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Liver Parenchyma
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Hepatocytes
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Liver Lobule
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Pancreas
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Pancreas
Exocrine: acinar cells
Endocrine: islet cells
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Pancreas
Acinar Cells
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Large Intestine(Colon)
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Appendix
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Appendix
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Colon
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Colon
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Glands in Colon
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Glands in Colon
colon polyp
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PhysioEx 39B
Experiment 1: Starch Digestion
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Starch Digestion by AmylaseStarch is a polymer composed of repeating units of glucose.
Amylase breaks starch into smaller subunits such as maltose.
Maltose
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Load 7 tubes
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Fill 7 tubes,use droppers
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Boil tube 1,Incubate tubes
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Transfer liquidto new tubes
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Add IKI reagent
Add Benedict’s reagent and incubate
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Record data
Print data table and answer questions as directed in your Lab manual
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End Lab 8
Please bring a full bladder to lab next week for urinalysis!