standard operating procedures to conduct research

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1 TUSKEGEE UNIVERSITY CAPRINE RESEARCH AND EDUCATION UNIT STANDARD OPERATING PROCEDURES TO CONDUCT RESEARCH Research Faculty Area of Specialization Nar Gurung Acting Director, Animal Nutrition Olga Bolden-Tiller Reproductive Biology Chuck Okere Animal Breeding/Reproduction Jannette Bartlett Animal Nutrition, Immunology Errol Rhoden Agronomy, Forages William Witola Molecular Parasitologist Byeng-ryel Min Animal Nutrition/Rumen Microbiology Wendell McElhenney Biostatistics/Animal Breeding Jesse Jaynes Molecular Biology Deloris Alexander Molecular Biology Uma Karki Forage Ecology Norma Dawkins Food Science Nii Tackie Agriculture Economics Initiated: January, 2013 Completed: June, 2013 Approved By: _____________________________________________________________ Dr. Conrad Bonsi, Associate Director, George Washington Carver Agricultural Experiment Station _____________________________________________________________________ Dr. Walter Hill, Vice Provost and Dean, College of Agriculture, Environment and Nutrition Sciences; Director, George Washington Carver Agricultural Experiment Station and Administrator, 1890 Tuskegee University Cooperative Extension Programs

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TUSKEGEE UNIVERSITY CAPRINE RESEARCH AND EDUCATION UNIT

STANDARD OPERATING PROCEDURES TO CONDUCT RESEARCH

Research Faculty Area of Specialization

Nar Gurung Acting Director, Animal Nutrition

Olga Bolden-Tiller Reproductive Biology

Chuck Okere Animal Breeding/Reproduction

Jannette Bartlett Animal Nutrition, Immunology

Errol Rhoden Agronomy, Forages

William Witola Molecular Parasitologist

Byeng-ryel Min Animal Nutrition/Rumen Microbiology

Wendell McElhenney Biostatistics/Animal Breeding

Jesse Jaynes Molecular Biology

Deloris Alexander Molecular Biology

Uma Karki Forage Ecology

Norma Dawkins Food Science

Nii Tackie Agriculture Economics

Initiated: January, 2013 Completed: June, 2013

Approved By:

_____________________________________________________________

Dr. Conrad Bonsi, Associate Director, George Washington Carver Agricultural

Experiment Station

_____________________________________________________________________

Dr. Walter Hill, Vice Provost and Dean, College of Agriculture, Environment and

Nutrition Sciences; Director, George Washington Carver Agricultural Experiment Station

and Administrator, 1890 Tuskegee University Cooperative Extension Programs

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TABLE of CONTENTS

SECTIONS Page

OVERVIEW 3

PURPOSE 3

SECTION I. GENERAL PROCUDURES TO CONDUCT RESEARCH 4

A- Prior to Conducting Research 4

B- During the Research 5

C-Facility Equipment Use 6

D- Facility Use 6

E. Sample Storage 7

F. Bio-Security Measures 7

SECTION II. QUARANTINING ANIMALS 8

SECTION III. MANAGING SICK ANIMALS AND DURING EMERGENCIES 9

A. Sick Animals 10

B. Emergencies 10

1. Animal Health Emergency 10

2. Fire Emergency 11

3. Other Emergencies 12

SECTION IV. FORAGE PROGRAM AT THE CAPRINE RESEARCH UNIT 12

SECTION V. MONITORING AND CORRECTIVE ACTIONS 13

A. Monitoring 13

B. Corrective Actions 13

C. Verification and Record Keeping 14

SECTION VI. SPECIAL REQUIREMENTS 15

A. Sheep Research 15

B. Co-grazing Research 15

C. Scrapie Eradication Program in Alabama 15

APENDECIS

A. CAPRINE RESEARCH UNIT FORAGE/PASTURE STATUS 16

B. VISITOR GUIDELINES FOR THE TUSKEGEE UNIVERSITY

CAPRINE RESEARCH UNIT 17

C. STANDARD OPERATING PROCEDURES GOVERNING

MANAGEMENT OF SEED HERD 18

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D. OUTLINE FOR BREEDING AND PERFORMANCE TEST 20

E. CAPRINE RESEARCH UNIT SOP ASSESSMENT TEST 23

OVERVIEW

The College of Agriculture, Environment, and Nutrition Sciences (CAENS) and the

George Washington Carver Agricultural Experiment Station (GWCAES) recognized that

the Tuskegee University Caprine Research and Education Unit (TUCREU) needs a

standard operating procedure (SOP) to promote human treatment of animals and the proper

care of the animals used in agricultural research and teaching according to the Institutional

Animal Care and Use Committee Guidebook (ARENA/OLAW, 2002), the Public Health

Service Policy on Humane Care and Use of Laboratory Animals (PHS, 2002); to conduct

the highest standard of goat research, outreach and education, as it relates to the Unit.

Therefore, this document was written to ensure high standard and quality within our

research programs, providing standardized guidelines for management, research, outreach,

and teaching activities at the TUCREU. The SOP defines the basic high standards for

quality goat research at Tuskegee University, “showcasing” the university at the regional,

national as well as international level. This SOP will be reviewed and updated as

warranted.

PURPOSE

This document is the “Standard Operating Procedure” (SOP) and is to be used as a guide

for all persons conducting research and other activities at the Tuskegee University Caprine

Research and Education Unit (TUCREU). This document describes the policies and

procedures for planning and implementing research and is designed to inform users of the

standardized procedures to be adhered to while performing routine activities at the

TUCREU. The development and use of this SOP is an endeavor to standardize and

implement quality research procedures as part of the operations of the Unit in an effort to

maintain the integrity of work conducted at TUCREU. This SOP describes the technical

and administrative operational elements of Tuskegee University Caprine Research Unit. In

addition, the SOP details the process that is followed in conducting research at the

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TUCREU. This SOP is designed for the successful conduct of research at TUCREU.

In this document the TUCREU, CRU or Unit refer to the Tuskegee University Caprine

Research Unit; the Director refers to the Director of Small Ruminant Research Program;

and attending veterinarian refers to any veterinarian working with TUCREU or on a related

project. However, Attending Veterinarian refers to the official Tuskegee University

Attending Veterinarian.

SECTION I: GENERAL PROCUDURES TO CONDUCT RESEARCH

PURPOSE: This SOP serves as a guideline for conducting research at the TUCREU.

SCOPE: The TUCREU provides research opportunities to individuals who are

associated with programs funded through the George Washington Carver

Agricultural Experiment Station (GWCAES), which operates and maintains

the facility, and others.

PROCEDURES

A- Prior to Conducting Research

1. File a Project Outline Form (POF) and submit to the Experiment Station via the Director of

Small Ruminant Research Program (DSRRP). The document must be submitted prior to

the start of each research cycle*. Research cycles begin September 1 or February 1 of each

year. The purpose of POF is follows:

a. To coordinate research at TUCREU,

b. To prioritize research to be done at the TUCREU, and

c. To reduce duplication of research objectives.

*Within two weeks of the start of each research cycle, all persons submitting

projects, the DSRRP, and either the Director or Associate Director of the

GWCAES will review the project outlines to finalize the research activities for

that research cycle (See A.1. above).

2. Researchers are responsible for securing funding for the following:

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a. Experimental animals, feeds, grain, hay, pasture, etc.

b. Supplies such as trashcans, feeders, and medical supplies. However, if supplies

are available at the TUCREU, they can be used upon request from the unit manager.

3. Submit an approved Tuskegee University Animal Care and Use Committee (TUACUC)

form.

4. Upon approval of research activities, the Unit manager (via the DSSRP)

will confirm the following as related to each research project:

a. Secure place in the barn to house research animals.

b. Secure pasture to house research animals.

c. Assist researchers, including securing animals and feed and other services needed for

research.

B- During the Research

1. Researchers and all personnel must follow guidelines for animal care and

SOP; failure to do so may result in suspension of research activities.

2. Attending personnel must be trained for animal care at the TUCREU.

3. Researchers should identify experimental animals, pens and pastures by:

a. Title of research.

b. Name of Advisor/Researcher with contact telephone number.

c. Name of the attending personnel with contact telephone numbers.

d. TUACUC approval number.

e. Attending veterinarian on the project with contact telephone number.

4. Researchers and students are responsible for:

a. Feeding.

b. Sample collection.

c. Cleaning the facilities.

d. Research animal health.

C-Facility Equipment Use

1. All equipment belonging to the Unit will be labeled TUCREU.

2. Unit manager must be informed prior to the use of all TUCREU equipment.

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3. Researchers must complete the Equipment Need form; priority will be

given to graduating student’s research or on a first come first served basis.

4. All equipment purchased for TUCREU by the GWCAES will be housed at

the Unit and cannot be removed from the Unit without prior permission from

the Unit manager or DSRRP.

5. Researchers needing to house specialized equipment not provided by the

unit may do so upon informing Unit manager (necessary for space management

and security).

6. Researchers should inform the Unit manager about equipment and facility

repair needed immediately.

7. Researchers must inform Unit manager to dispose of unused material.

8. All TUCRU equipment and supplies not reserved for use can be used for

research projects upon informing the Unit manager.

D- Facility Use

1. The Director and Unit manager must be informed in advance of all research,

teaching and outreach activities to be conducted at the Unit.

2. Indoor facilities are limited and priority of research will be given to

graduating students’ research whose research projects have been properly

submitted (See Section I. A. 1.).

3. Number of animals used for research in the facility must be minimized.

4. Number of pastures and size are limited and multi pasture use by any one

investigator must be discussed with the DSRRP.

5. Efficient use of resources, collaboration and multi-disciplinary research is

encouraged and favored.

E. Sample Storage

1. All samples must be properly labeled – date/name of principal

investigator/name of student/type of sample.

2. Samples may not be stored longer than 12 months and must be removed to

a permanent location at the end of each research project as defined on the

project outline.

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3. The Director will inform the researchers in writing to remove samples

stored for more than 12 months. After three attempts, they will be removed

by Unit manager and dispose of properly.

F. Bio-Security Measures

Bio-security measures are necessary to protect animals and persons working

with animals from harm.

1. Protective measures for persons while working with animals include:

a. Protective attire (proper work clothes (uniform), lab coats,

coveralls, boots, gloves, masks, etc) should be worn at all times

when working with animals.

b. Work clothes should be washed and can be housed in assigned

lockers at the TUCREU laundry facility.

2. Protective measures for animals include:

a. Limiting animal contact from non-TUCREU personnel

b. New animal arrivals should be quarantined for at least 21 days before adding to the herd

(See Section II). The quarantine facilities should be designed to minimize the risk of

introducing or transmitting diseases agents within the same species or between species.

c. Use foot-baths before entering TUCREU barn or pastures.

d. Use separate equipment for outreach activities, especially when visiting/working on other

farms if possible. All common use equipment must be thoroughly disinfected before

returning to the TUCREU.

e. Clean and disinfect equipment used for transportation of animals after each use.

f. Clean and disinfect ALL common equipments to prevent contamination between users

within the unit.

SECTION II: STANDARD OPERATING PROCEDURE FOR QUARANTINING ANIMALS

BROUGHT TO THE TUSKEGEE UNIVERSITY CAPRINE RESEARCH AND

EDUCATION UNIT

PURPOSE: To ensure that the Caprine Research Unit maintains a healthy environment

free from diseases and parasites, when goats are brought in from an external source,

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strict measures (outlined below) should be taken to prevent bringing harmful

microorganisms to the Unit. The use of sheep for research or for teaching purpose

at the TUCREU shall follow the similar guidelines.

SCOPE: Researcher(s) are responsible to follow these procedures:

PROCEDURES

A. Goats coming to the Caprine Research Unit from an outside source should have a

certificate showing the last physical examination (deworming, vaccinations, any other

health problems, etc.) from the producer (whether done by a veterinarian or the

producer himself/herself) and must be accompanied by a health certificate from a

certified veterinarian. This certificate may also be obtained from the TUCREU

attending veterinarian upon examination of the animal(s) while under quarantine.

Alabama Department of Agriculture and Industries Administrative Code 80-3-6-.18

now also requires all goats and sheep to be identified with official Scrapie ID prior to

change of ownership or transport to fairs, exhibitions or across state lines.

B. Goats should be free from diseases and other illnesses before use at the research

site. There should be no obvious signs of any health problems.

C. Goats should be placed in a separate holding facility, designated the “quarantine

area,” away from the barn and any other goats already present at the Unit.

1. The holding facility can be a pasture (more than one) set

aside for quarantine purposes only, or

2. A building constructed at least 200 yards from the main barn for

Quarantine purposes only.

3. New arrivals must have access to suitable quality feed and adequate supply

of clean water.

D. Within the first week of entering the quarantine facility, animals should be

evaluated by an attending veterinarian and treated if needed. The following should be

taken into consideration:

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1. Body temperature,

2. Body condition score,

3. Eye color,

4. Physical deformities,

5. Deworming if necessary,

6. Vaccinations if necessary, and

7. Others as needed.

E. Ill animals as determined by physical examination by the TUCREU attending

veterinarian, should immediately be removed and placed in an area set aside for

sick animals only (“sick bay” area). These animals should be treated separately

until they are well enough to join the rest of the group.

F. Complete paperwork from the veterinarian doing the physical examination

should be maintained at the TUCREU facility.

SECTION III: STANDARD OPERATING PROCEDURE FOR MANAGING SICK ANIMALS

AND DURING EMERGENCIES

PURPOSE: To prepare an appropriate contingency plan for the TUCREU for effective

implementation of the guidelines to provide an appropriate standard of care for the sick

animals, and during emergencies, including disasters and evacuations.

SCOPE: This procedure applies to all TUCREU personnel and users.

PROCEDURES

A. Sick Animals

1. Goats that are obviously sick, injured, or disabled must be immediately

separated from the healthy goats.

2. The TUCREU must have designated pen(s) for sick animals.

3. Sick animals should be handled according to the TUACUC or the Guide

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for the Care and Use of Agricultural Animals in Agricultural Research and

Teaching, which is available at the TUCREU office. Equipment should be

used to move non-ambulatory animals within the Unit to prevent undue

suffering.

4. All persons attending sick animals must wear boots or clothing, including

appropriate safety gloves.

5. The unit attending veterinarian should be contacted for appropriate

treatment plans for the sick animals.

6. Do not use sick animal cages or pens as thoroughfares and notify the

TUCREU manager IMMEDIATELY to ensure that proper cleaning

procedures are performed.

7. Do not transport a sick or injured animal where undue suffering will

result or when parturition is imminent. Goats considered suffering

severe distress or injury should be euthanized on site and under no

circumstances should be transported to livestock auction markets or over

long distances to meat packers.

8. When possible, all dead animals should undergo a postmortem

examination by a qualified veterinary pathologist.

9. Disinfect the pens or cages following the removal of the sick animals.

10. Report all problems to the TUCREU manager, DSRRP, attending veterinarian, or the

Director of the Comparative Medicine Resource Center on campus.

B. Emergencies

Disasters such as hurricanes, tornadoes, earthquakes, fires, disease outbreaks, etc.

can happen at any time. Written plans should be kept in a fireproof safe with the

other important documents for quick accessibility. Procedures are as follows:

1. Animal Health Emergency

a. Any type of serious health problems should be treated as an

“emergency,” and above procedures (See Section III.1.) should be

followed.

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b. The TUCREU manager, DSRRP, Attending Veterinarian or the

Director of the Comparative Medicine Resource Center should be

IMMEDIATELY notified. Emergencies cannot wait until the start

of the next business day, including overnights and weekends.

c. Inform the principal researcher(s) or collaborators by phone.

Phone numbers, including office and emergency contact information

should be posted within the unit.

d. The list of the telephone numbers for the TUCREU manager,

DSRRP, TUCREU attending veterinarian, Tuskegee University

Attending Veterinarian, and the Director of the Comparative

Medicine Resource Center must be clearly accessible within the

unit.

e. Dead goats, placentas and aborted fetuses, offal, etc., must be

removed immediately and disposed of according to appropriate

regulations set by TUACUC or the Guide for the Care and Use of

Agricultural Animals in Agricultural Research and Teaching.

2. Fire Emergency

a. In case of fire, contact the local fire service OR CALL 911.

b. A list of telephone numbers of local, state and federal groups

that could provide assistance should be easily accessible.

c. In case of extended power outage, on duty personnel should

relocate samples stored in refrigerators to a secure area. Dead

carcasses should be emptied from the freezer(s) and properly

disposed of immediately.

3. Other Emergencies

a. Other emergencies may include hurricanes, tornadoes,

earthquakes, and unavoidable facility problems such as temperature

or humidity extremes etc. The following persons should also be

notified immediately: Researchers, Unit manager, DSRRP or the

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Attending Veterinarian or the Director of the Comparative Medicine

Resource Center. Call 911 if appropriate.

b. Assess the prevailing conditions and decide whether to evacuate

or stay in the building in “safe areas.”

c. ”Safe areas” should be identified in advance and employees

should be regularly instructed as to the location of the safe area.

SECTION IV: STANDARD OPERATING PROCEDURES FOR THE FORAGE

PROGRAM

PURPOSE: To maintain high quality forage and proper herd health at the TUCREU.

Carrying capacity of the Unit and number of animals that will meet the research, teaching,

and outreach needs of faculty, staff and students at Tuskegee University will be

considered.

SCOPE: All goat-pasture related researcher(s) and the goat Unit manager are

responsible for following these procedures.

PROCEDURES

Space availability must be considered when determining use of pastures and maximum

carrying capacity of the pastures (See Appendix A). The TUCREU manager will oversee

the maintenance of forage program and confer with specialists as needed to ensure that

sufficient forage is available for scheduled research.

The following guidelines will be followed:

A. Total animals to be carried on the farm pastures are not to exceed 150.

B. Total animal numbers maybe adjusted according to pasture carrying capacity.

C. All fields must be soil tested in the fall and lime applied according to soil test recommendations.

D. In spring, half of the fertilizer recommended will be applied and remainder by early July.

E. When forage exceeds the consumption of the animals at the Unit, it will be cut and baled.

SECTION V: STANDARD OPERATING PROCEDURES FOR MONITORING

AND CORRECTIVE ACTIONS

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PURPOSE: This section deals with monitoring SOP. Failure to follow SOP may result

in delay and suspension of research or lose of privileges of using the

TUCREU.

SCOPE: This will apply to all research conducted at the Unit and includes any

violations of SOP by researchers, staff and students.

PROCEDURES

A. Monitoring

The Director of Small Ruminant Research Program or a trained designee will monitor the

SOP procedures to be followed.

B. Corrective Actions

1. If procedures are not followed, the Director of the Small Ruminant Research

Program will discuss inadequacies with the concerned parties to ensure that the

procedures are followed.

2. Uncorrected inadequacies will be addressed by the DSRRP and will be

communicated with the Associate Director of George Washington Carver

Agriculture Experiment Station and may result in suspension of research activities

for the remainder of the research cycle.

3. Inadequacies related to animal care will be reported to the DSRRP and the TU

Attending Veterinarian.

4. The DSRRP will report ALL problem/s in writing to the Director of George

Washington Carver Agriculture Experiment Station (GWCAES) through the

Associate Director of GWCAES.

C. Verification and Record Keeping

The Director of the Small Ruminant Research Program or a trained designee will record

the inadequacies and any corrective actions taken on the SOP, and will inform Associate

Director of GWCAES.

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SECTION VI. SPECIAL REQUIREMENTS

A. Sheep Research

Sheep and goats have often similar care and management needs but since they are

different species they have several physiological differences between them. Their

foraging behaviors are very different. Goats are considered browsers while sheep are

termed as grazers. Similarly, their response to fencing is different. Goats are very

agile and curious animals so they are very difficult to contain. While many diseases

and parasites are common to both sheep and goats, there are some important differences

between the two species. Therefore, they should be kept separately during the quarantine phase as

well as while using for research and teaching purposes. The distance between the two species

should be based on the recommendation of the Animal Care and Use Committee.

B. Co-grazing Research

Some research projects require that beef cattle and goats be grazed together. This work

must be done for the minimum amounts of time necessary to accomplish the research

objectives. The livestock species that are used for the projects should be subjected to the

standard quarantine procedures if they are purchased from outside. The cattle

quarantine procedures should be followed according to the Animal Care and Use

Committee guidelines. Cattle that are used for co-grazing research should have calm

dispositions and be adapted to the frequent contacts with animal care takers and

research personnel. The grazing plots should have adequate provision of shades for both

species to minimize stresses from extreme heat and cold. Fresh water must be made

available at all times and water space must be adequate to allow for sufficient room for

all animals to access and benefit from available water.

C. Scrapie Eradication Program in Alabama

A new rule passed recently by the Alabama Department of Agriculture will apply to goat

producers across the state. Alabama Department of Agriculture and Industries

Administrative Code 80-3-6-.18 now requires all goats and sheep to be identified with

official Scrapie ID prior to change of ownership or transport to fairs, exhibitions or across

state lines. The previous version of the rule only required official ID of all sheep. USDA

and State efforts are underway at this time to educate goat owners and producers about the

rule change. The rule will begin to be enforced on January 1. 2013. Producers can pick up

Scrapie Brochures and tag order forms at most stockyards, some county agent offices, and

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feed stores throughout Alabama. Producers can also call (866)USDA TAG to request the

brochure and tag order form. It takes approximately 3 to 4 weeks for the producer to get

their tags after the tag request is received by the USDA /State Office. Registered goats that

are identified with a legible registry assigned tattoo and the goat is accompanied by a copy

of their registration papers do not have to have a Scrapie tag installed. In some cases

microchips are acceptable as official Scrapie ID.

Alabama Rule 80-3-6-.18

(4) All sheep and goats that move within the state that involves, but not limited to, change

of ownership, shows, fairs, expositions or slaughter shall be permanently individually

identified by method approved in 9 CFR Parts 54 and 79 allowing the animal(s) to be

traced to flock of origin.

Authors: Robert J. Russell, Reginald L. Sorrells

Statutory Authority: Code of Ala. 1975, §2-15-170.

APPENDIX A

CAPRINE RESEARCH AND EDUCATION UNIT FORAGE/PASTURE STATUS

1. Total paddocks at the time of finalizing this SOP (January, 2013)) are 38 with

additional paddock may be constructed as needed.

2. 14 plots are about 2 acres each while 24 plots are 0.5 acres each.

3. Total area for pasture/browse at present time is 36 acres with additional 12 acres

constructed last year

4. Assuming carrying capacity of 5 goats per acre, the total number of animals on

Pastures/browse will be equal to 180 animals. However, we have set our

maximum capacity of 150 animals.

5. Six paddocks are reserved for seed herd development.

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APPENDIX B

VISITOR GUIDELINES FOR THE TUSKEGEE UNIVERSITY CAPRINE RESEARCH AND

EDUCATION UNIT

The Tuskegee University Caprine Research Unit attracts many visitors (producers,

researchers, students, etc.) every year. The unit is open to the general public if prior

approval is obtained from the unit manager or DSRRP. While the majority of visits to the

unit will be problem-free, there are various safety issues to be considered. For the safety of

the visitors and animals, we request that the following guidelines be strictly followed:

o All individuals and groups visiting the unit must obtain prior approval from the unit

manager or director.

o For your safety, please do not make any sudden movements, which may startle and

frighten goats.

o For your safety, shouting, screaming, running and touching animals is strictly

prohibited.

o Don't touch any machinery at the unit.

o Please wash your hands if you touch anything at the unit.

o Don't let children pick up and eat anything that's fallen on the ground, such as

apples or other fruits.

o Eating and drinking at Caprine Research Unit is strictly prohibited except in the

designated areas.

o Don't drink water from taps, unless they are marked for human use.

o Visitors are not allowed to bring animals onto the Tuskegee University Caprine

Unit without permission from the unit manager or director and absolutely NO

VISITING ANIMALS will be allowed onto the Caprine Research Unit Facilities.

o Visitors must wear proper gear, including gloves, masks and eye protection, prior

entering Caprine Research Unit Facilities if requested by the unit manager or

his/her designee.

o The manager or his/her designee must accompany visitors at all times.

Failure to adhere to any of the rules may result in permanent banishment from the

Caprine Research and Education Unit.

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STANDARD OPERATING PROCEDURES GOVERNING MANAGEMENT OF SEED HERD

Pre-determined number of goats of specific breeding will be maintained at the TUCREU as

a seed herd. Present seed herd consists of 10 purebred Boer doelings, 8 purebred Kiko

doelings, 2 purebred bucks one of each breed and two companion wethers. Seed herd is

maintained on six paddocks with no cross contamination with commercial herd and serves

for breeding purposes only. No invasive research will be conducted with seed herd except

for those designed for management practices. Following applies to seed herd:

● Seed herd will be bred and used for breeding purposes and for increasing the herd number.

● Purebred animals will be maintained at the Unit at all times.

● Pre-determined cross breeding will be practiced on seed herd to produce composite breed

for future purposes.

● Seed herd will be kept separately from rest of the commercial herd at all times.

● Animals purchased for seed herd will be quarantined in separate areas than those used for

commercial herd.

● Commercial herd will be used for research and purebreds will be used for research if

available. If seed herd animal is used for research with commercial herd it will be

considered commercial herd and will not return to the seed herd.

● Seed herd will not graze areas that have been predisposed to commercial herd.

Health Monitoring

● Seed herd will be monitored for parasite control by checking fecal egg counts and

FAMACHA eye color chart system every 3-4 months or as needed.

● Seed herd will be maintained with proper vaccinations at all times.

● Pasture rotation will be practiced to maintain proper forage quality and animal

performance. Extra forage will be bailed as hay.

Performance Monitoring

● Performance of seed herd will be monitored every 4 weeks.

● Animals should be weighed every 4 weeks.

● Average daily gain should be monitored.

● Feed intake should be monitored if possible.

● Amount of grain or hay fed per group should be recorded.

● Stocking rates and days grazing a specific paddock should be recorded.

● Feed efficiency should be monitored.

● Animals should receive fresh water and mineral mix at all times.

Reproductive Performance

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● Duration of time that animals were exposed to buck should be monitored.

● Pregnancy rates should be recorded.

● Number of kids/doe and kids’ weight should be recorded every 4 weeks.

● Weaning weight should be recorded.

● Number of kids weaned per doe should be recorded.

● Average daily gain for kids should be recorded.

Kid Health Monitor

● Kids should be weighed at birth.

● Kids should be vaccinated with proper vaccines as recommended by veterinarian.

● Dehorn at less than 1 month of age for ease of the procedure and effectiveness of removing

all of the horn bud (AG Guide, 2010). Cautery with heat should be the method of choice

when possible, although surgery, freezing, and an acidic paste are other options)

● Castrate if needed when kids are between 24 h and 7 d of age. This is a common

recommendation. Detailed descriptions of castration procedures are available in various

publications (ASIA, 2002; Greiner and Wahlberg, 2003; Faerber, 2004)

● Make sure the doe accepts her kid (s) for nursing.

● Be prepared to feed colostrums if needed. You may purchase ready colostrums for orphan

kids. Or you may milk the doe for first 3 days and feed it to kids.

● Have some kid bottles ready if needed.

PLEASE READ “SIMPLY MEAT GOATS” FOR FURTHER INFORMATION

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APENDIX D

OUTLINE FOR BREEDING AND PERFORMANCE TEST

I. Inventory

A. Add does and bucks to inventory and ID the does leaving the herd and reason for disposal

II. Breeding

A. Record input

i. Sire

ii. Dates bred or time exposed

iii. Date or result of pregnancy tests

B. Record analysis output

i. Expected kidding dates

ii. Percent of bred to those exposed for each buck and entire herd

III. Kidding

A. Record input

i. Birth date

ii. Birth weight

iii. Kidding ease

iv. Kid ID

v. Buck and Doe ID

B. Record analysis output

i. Adjusted birth weight and ratio

ii. Kidding ease (separate for first kidding doelings and older does)

iii. Gestation length adjusted for doe age and sex of kid

iv. Proportion of kidding for each 21 days of kidding season

v. Percent kid crop of those exposed

vi. Birth weight and kidding ease

vii. Kidding interval

IV. Weaning (kid age range of 84-133 days)

A. Record input

i. Date weighed

ii. Kid weight

iii. Contemporary group average weight

iv. Doe weight, hip height and condition score

v. Doe pregnancy status

B. Record analysis output

i. Adjusted 108 day weaning weight and ratio

ii. Repeat of kidding information

iii. Herd mate difference

V. Yearling (Age range of 330-390 days)

A. Record input

i. Dates weighed and measured

ii. Weight, hip height and scrotal circumference

iii. Contemporary average measurements

B. Record analysis output

i. Adjusted yearling weight ratio

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ii. Repeat of kidding and weaning

C. Selection decisions

i. Select bucks by comparing the breeding values for each trait for sire and yearling

ii. Cull doelings that are structurally unsound and too extreme in frame (large or small)

VI. Contribution to commercial herds

A. Record input

i. Production costs for weaned kids

ii. Carcass weight, selection grade

iii. Measures of tenderness and palatability

B. Record output

i. Breakdown prices for weaned kids

ii. Expected progeny difference for carcass weight

iii. Expected progeny difference for quality measurements

C. Selection decisions

i. Select bucks that progeny excel in low breakdown price

ii. Select bucks whose progeny excel in optimum carcass weights and quality

KIDDING EASE SCORES

1. No difficulty, no assistance

2. Minor difficulty, some assistance

3. Major difficulty, usually mechanical assistance

4. Caesarean section or other surgery

5. Abnormal presentation

ADJUSTED WEANING WEIGHT

Adjusted 108 day wt. lbs. = Actual Weaning Wt. – Birth weight x 108 + Birth Wt.+ Age of

Age in days doe adjustments

AGE OF DOE ADJUSTMENTS for BIRTH WT. and WEANING WEIGHT

Age of Doe Birth Wt. Adj. WW. Adj (lbs.)

Male Female

2 yrs and under 0.8 6 5

3 yrs 0.5 4 3.5

4 yrs 0.2 2 1.5

5-10 yrs 0.0 0 0

Older 0.3 2 1.5

WEANING WEIGHT RATIO

RATIO % = Adjusted Weaning Weight of Kid X 100

Average Adj. WWt. Of Contemporaries

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ADJUSTED YEARLING WEIGHT

Obtain data on 100- 150 days of post weaning gain

Adjusted 365 days weight = Actual final wt.- Actual weaning wt. x 257 + Adj. Weaning Wt.

Number of days in between

YEARLING WEIGHT RATIO

Yearling wt. Ratio % = Adjusted Yearling weight . x 100

Ave. Adj. Yearling

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CAPRINE RESEARCH AND EDUCATION UNIT SOP ASSESSMENT TEST

MULTIPLE CHOICES: Choose most appropriate answer.

1. ________The TUCREU SOP does which of the following: a. Serves as a guide for all persons conduct research and other activities at TUCREU.

b. Serves as a guide for all persons conduct research only at TUCREU.

c. Is designed to inform users of the standardized procedures to be adhered to while performing

routine activities at the TUCREU.

d. None of the above. e. A and C only.

2. ________Which of the following is a bio-security protective measure for animals?

a. Limiting animal contact from non-TUCREU personnel b. Do not use foot baths before entering barns and pastures.

c. Add new animal arrivals directly to the working herd if they look healthy. d. None of the above.

e. A and C only.

3. ________Concerning research animals and facilities during active research projects, the unit

manager and unit personnel are solely responsible for which of the following:

a. Feeding. b. Sample collection.

c. Cleaning the facilities.

d. Animal health. e. A, B, and C only.

f. None of the above.

4. ________Which of the following is not a priority of the Research Project Outline which should be

submitted prior to conducting research. a. To coordinate research at TUCREU

b. To prioritize research to be done at the TUCREU

c. To reduce duplication of research objectives d. All of the above.

e. A and C only.

5. ________ Within the first week of entering the quarantine facility, animals should be evaluated for

which of the following: by an attending veterinarian.

a. Body temperature b. Body condition score

c. Eye Color

d. Physical deformities e. All of the above

6. In case of an emergency, such as a fire, who should be contacted? a. TUCREU manager

b. DSRR

c. Attending Veterinarian/Director of the Comparative Medicine Resource Center d. Campus operator

23

e. Security

f. 911 g. Inform the principal researcher(s) or collaborators

h. All of the above.

i. A and F only. Emergencies cannot wait until the start of the next business day, including

overnights and weekends. TRUE/FASE

7. ________ There is a specific set of guidelines for all visitors (non-personnel) to the TUCREU.

8. ________ Serious health issues do not need to be considered as “emergencies” and it is okay to

wait until the next business day to notify the principle investigator or other appropriate personnel.

9. ________Sick or injured animals may remain with healthy animals as long as they are being

properly medicated.

10. ________A high quality forage program is important for maximizing capacity at the TUCREU.

11. ________ Failure to following the SOP may result in suspension of research activities.

12. ________New animal arrivals MUST be quarantined for at least 21 days.

13. ________ Within the first week of entering the quarantine facility, animals should be evaluated by

an attending veterinarian.

14. ________ The “quarantine area” must be at least 100 yards away from the barn and any other goats

already present at the Unit and may be a pasture or a building.

15. ________ The sick bay refers to the holding area for ill animals as determined by the attending

veterinarian.

References:

1. ARENA and OLAW. 2002. Institutional Animal Care and Use Committee Guidebook. Department

of Health and Human Services. Washington. DC.

2. ASIA (American Sheep Industry Association). 2002. Sheep Production Handbook. ASIA.

Centennial. CO.

3. Faerber, C. W. 2004. Small Ruminant Production Medicine and Management: Sheep and Goats. 3rd

Ed. Animal Health Publications. Preston. ID.

4. Greiner, S. P. and M. L. Wahlberg. 2003. Newborn Lamb Management. Virginia Cooperative

Extension Publication Number 410-026. September 2003. http://www.ext.vt.edu/pubs/sheep/410-

026/410-026.html. Accessed January 10, 2013.

5. PHS. 2002. Public Health Service Policy on Humane Care and Use of Laboratory Animals.

Department of Health and Human Services. Washington. DC.