standard data analysis - calibration chemstation – level 1 - training
Post on 21-Dec-2015
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Standard Data Analysis -Calibration
ChemStation – Level 1 - Training
1. Inject standards
2. Set-up appropriate integration events for the low standard.
3. Begin a new calibration table with level one, the lowestlevel. Fill in the dialog.
4. Integrate each additional standard level and add to the calibration table.
5. Examine the calibration curve and save it to the method.
Building a Calibration Table
Example Calibration
1. Standard 30.0 ISTD 50.02. Standard 50.0 ISTD 50.03. Standard 75.0 ISTD 50.0
ng/L ng/L
3 Levelsmin1 2 3 4 5 6
mAU
0
20
40
60
80
100
DAD1 A, Sig=254,4 Ref=550,100 (DEMO\005-0101.D)
0.7
47
1.0
21
2.5
65
5.8
37
Standard ISTD
Define signals to be evaluated during a method.
Signal Details
Calibration Settings
Load the Signal(s) for the Low Level Standard and Integrate
Create a Calibration Table: Level One
Fill in:Compound NameGroupAmount
Check if:Reference PeakISTDIndicate #
Calibration Table Overview: Fill in the Dialog
Load the Signal - Add Level Two
Add Additional Levels
Compound, Amt, Area, Rsp Factor,Ref, ISTD#
Compound, Grp, Amt, Low LimitHigh Limit
Compound, Amt, Area, Def, CurveType, Origin
Compound, Area, Rsp%, Def, +-, Pk Usage
Calibration Table Options
Use Low and High Limits as qualifiers to confirm peak
identification.
Compound Details
Check Def to apply thedefault curve type and origin from the Calibration Settingsdialog box.
Curve TypesPiece wise ExponentLinear QuadraticLog CubicPower Average Rsp/Amt
OriginIgnoreIncludeForceConnect straight segment
Peak Details
Define the columns included in your Calibration Table
Edit Calibration Table Options
Calibration TaskTool New Calibration
Table
Recalibrate withCurrent Chromatogram
Add New Level Signal Details
Add New Peaks
Select Calibrated peak(s) fromChromatogram and delete fromcalibration table.
Select peak(s) from chromatogram and addto calibration table
Select compoundsfrom chromatogram and recalibrate
Edit CalibrationTable Options
Quantification Tools
Save Table to Method
To Save the Calibration Table as Part of a Method...
Signal Options
Customized display on screen and in reports
Annotations
Use it for a customized chromatogram in slides or publications
Easy placing and moving of annotations
Selectable fonts and colors
The Annotation sidebar can be activated within all toolsets
Signal Manipulations
Overlay, subtract and align signals
Lab Time !!!! Lab 7 - Calibration