stall and field trials for the harmonization of acaricidal registration. joão r. martins. wgpr/vprg...
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Stall and field trials for the harmonization of acaricidal registration.
João R. Martins.
WGPR/VPRG Meeting
Campo Grande, 26-28 October, 2005
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Teste de estábulo
Bovinos em boxes Bovinos em boxes individuaisindividuais
3 Infestações semanais3 Infestações semanais4 semanas consecutivas4 semanas consecutivas
Tratamento e coleta diária de carrapatos
Metodologia
básica
Infestações semanais após o
Tratamento > poder de proteção
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Infestações: 250 mg de larvas
(aproximadamente 5000 larvas)
Teste de estábulo
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Teste de estábulo:
-bovinos permanecem em piso ripado
para facilitar a colheita dos carrapatos
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Teste de estábulo:
Carrapatos adultos ficam retidos na bandeja e são recolhidos diariamente
Registra-se o número e peso
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FÓRMULA PARA CÁLCULO DA FÓRMULA PARA CÁLCULO DA EFICÁCIA CARRAPATICIDAEFICÁCIA CARRAPATICIDA
média grupo controle – média grupo tratado
média grupo controle
EFICÁCIA = X 100
Em função das Lactonas Macrocíclicas interferirem no tamanho dos carrapatos,
compara-se também o peso dos mesmos nos animais tratados e controle
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Box apropriado para coleta de carrapatos
(teste de estábulo, manutenção
colônias, etc...)
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Stall test: type of individual pen in use by Senasa, Argentina
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Portaria 48, 12 de maio de 1997Regulamento técnico para licenciamento/renovação
de antiparasitários
• Teste de estábulo:• 5 bovinos/tratamento• Infestações: 2500 larvas (7-21 dias) – 3 semanas• Coletas: -3 a +23 dias de ensaio• Alocação: ordem decrescente de produção de
teleóginas• Cálculo da eficácia: [1-(Ta x Cb) + (Tb x Ca)] x 100
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[1-(Ta x Cb) + (Tb x Ca)] x 100
Ta= nº médio de teleóginas nos tratados (+1 a 23)Tb= nº médio de teleóginas nos tratados nos 3 dias antes do tratamentoCa= nº médio de teleóginas nos controles (+1 a +23)Cb= nº médio de teleóginas nos controles nos 3 dias antes do tratamento
Cálculo da eficácia: “poderá ser empregada a fórmula”
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Determinação da viabilidade das teleóginas:
% inibição de reprodução:
(I.R.Controle – I.R. tratado) + (I.R. Controle) x 100
I.R.= (média peso massa de ovos + média peso teleóginas) x (% eclosão) x 20.000
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Data analysis: according to total number of ticks, total weight of ticksand index of reproduction
Also considering: average weight of ticks, eggs laid per tick incubated, weight of eggs laid per gram of tick and % egg hatching
% reduction in number of ticks dropping:Efficacy = 100* (1 – T/C)T=geometric mean treatedC = geometric mean controlGeometric mean = transformation to the natural log of (count + 1)
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Field test:
Objective: measure acaricide efficay at field conditions
and product behavior in immersion dips (physical/chemical properties in a certain period)