staining for conventional light microscopy
TRANSCRIPT
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Stain
StainingStain
Noun Verb
Noun
What is stain and staining?
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Unstained tissue Stained tissue
Why do we stain?
Why do we stain?
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Dr Farhana Taher Sumya Guided by: Prof Khondkar Manzare Shamim
Dept. of Anatomy, BSMMU
STAINING For Conventional Light Microscopy
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What should the audience be a able to do after attending this session?
1. Define stain and staining
2. Explain what is achieved with staining
3. Explain how staining for conventional light microscopy take place
4. Explain some basic terms on staining
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What should the audience be a able to do?
5. Explain the causes and solutions of the problems commonly faced in staining
6. Cite examples of specific tissue components stained with special stain
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Staining Technique
Vital
Supravital
Intravital
Nonvital
How can we stain?
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Supravital staining: the cells with the dark blue dots and curved linear structures
(reticulum) are reticulocytes.
How can we stain?
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Nonvital staining
How can we stain?
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T
T ST
T
T
S
M S
SA
How can we stain?
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T
SS
How can we stain?
Physical staining
Chemical staining
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Monochrome stain Dichrome stain
How many colours do we need
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MetachromasiaHow many colours do we need
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T
Acid (-)S
Base (+)T
Base (+)S
Acid (-)T
Acidophilic
Basophilic
Do we understand basophilic and acidophilic?
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Do we understand basophilic and acidophilic?
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T
Acid (-)S
Base (+)P(NH)
Base (+)S
Acid (-)DNA
Acidophilic
Basophilic
2
Do we understand basophilic and acidophilic?
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Acid (-)P(-cooh)
Base (+)S
Do we understand basophilic and acidophilic?
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Do we understand basophilic and acidophilic?
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Acidophilic or basophilic
Do we understand basophilic and acidophilic?
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Acidophilic or basophilic
Do we understand basophilic and acidophilic?
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Acidophilic and basophilic
Do we understand basophilic and acidophilic?
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Acidophilic or basophilic
Do we understand basophilic and acidophilic?
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Dark or pale
Does dark mean blue?
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Dark or pale
Does dark mean blue?
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Dark or pale
Does dark mean blue?
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Dark or pale
Does dark mean blue?
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Dark or pale
Does dark mean blue?
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Dark or pale
Does dark mean blue?
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Dark or pale
Does dark mean blue?
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Acidophilic and basophilic
Can we identify tissue types with naked eye?
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Can we identify tissue types with naked eye?
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Can we identify tissue types with naked eye?
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Inadequate staining
What problems do we face in staining?
Clearing by xylene
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Uneven staining
What problems do we face in staining?
Proper timemaintaining and dehydration
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Overstaining with hematoxylin
What problems do we face in staining?
AcidAlcohol
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Understaining with eosin
What problems do we face in staining?
Addingacetic acid
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Overstaining with eosin
What problems do we face in staining?
Washingwithaldehyde tap waterOr 95% alcohol
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Opacity
What problems do we face in staining?
Completedehydration
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Presence of black component in section
What problems do we face in staining?
Filteringstain
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Is routine staining enough?
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Collagen Fiber
H and E stain
Mallory stainMasson trichome
Is routine staining enough?
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Silver StainH and E stain
Reticular fiber
Is routine staining enough?
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H and E stain
Elastic fibre
Van gieson stain Weigert’s Resorcin fuchsin
Is routine staining enough?
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Goblet cell
H and E
Alcian bluePAS stain
Is routine staining enough?
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Glomerular basement membrane
PAS stainH and E stain
Is routine staining enough?
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Glycocalyx membrane of villus
H and EPAS stain
Is routine staining enough?
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H and E Mallory Azan
Islets of Langerhans
Is routine staining enough?
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Congo red
Amyloid
H and E
Is routine staining enough?
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Feulgen stains
DNA
H and E
Is routine staining enough?
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Fat stain (osmium tetraoxide)Fat stain (Oil red O stain)
Fat
H and E
Is routine staining enough?
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So, what did we discuss today?
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Thank You
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Golgi apparatus stains dark from heavy metal ions in nerve tissue
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