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Staige Davis Mentors: T’ai Roulston and Rosemary Malfi College Science Scholars University of Virginia

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Page 1: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

Staige  Davis  Mentors:  T’ai  Roulston  and  Rosemary  Malfi  College  Science  Scholars  University  of  Virginia  

Page 2: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

  Encapsulation  Response    Non-­‐specific   Melanin    Cytotoxic  cascade  

  Image  from  Smilanich  et  al.  2009  

Page 3: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

-­‐  Important  wildflower  and  crop  pollinators  

-­‐  Social:  Queen  and  worker  caste  

-­‐  Native  bees  (50  species  in  North  America)  

-­‐  6  species  regularly  occur  at  Blandy;  3  are  most  abundant  

Page 4: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

  Cameron  et  al.  2011:  Patterns  of  widespread  decline  in  North  American  bumblebees  

B.  pennsylvanicus   B.  affinis   B.  impatiens  

Page 5: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

10-­‐14  days   ~  1  year  

Conopid  life  history  diagramed  by  Amber  Slatosky.  

Page 6: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

  (1)  Do  rates  of  parasitism  by  conopid  flies  differ  across  bumblebee  species?      

  (2)  Do  conopid  larvae  achieve  successful  pupation  more  frequently  in  certain  bee  species  than  in  others?    

  (3)  Lastly,  is  the  encapsulation  response  to  foreign  objects  more  intense  in  certain  bumblebee  species  than  others?    

Page 7: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

“Frozen”  Treatment-­‐  measures  overall  parasitsm  rates  

“Natural”  Death  Treatment-­‐  

measures  rate  of  successful  pupation  

Page 8: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

  Freezer-­‐anesthetized    Implants  inserted  into  live  bees    Freezer-­‐killed  after  2  hours    Implant  extracted  and  photographed  

B.  bimaculatus   B.  griseocollis   B.  impatiens  

Page 9: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

 …coming  Spring  2014!  

Page 10: StaigeDavis’sciencescholars.clas.virginia.edu/public/presentations... · 2013. 10. 30. · Encapsulation Response’ Nonspecific Melanin’ Cytotoxic cascade’ Image’from’

  Mentors:  T’ai  Roulston  and  Rosemary  Malfi    Field  and  Laboratory  Assistance:  Ariel  Firebaugh,  

Amber  Slatosky,  Courtney  Beach,  Kellen  Paine    Many  thanks  go  to  the  generous  donors  to  the  College  

Science  Scholars  program  who  make  these  summer  research  awards  possible:  Charles  Henry  Leach,  II,  Foundation,  Mr.  Steve  Van  Besien;  Entigence  Corporation;  Lois  A.  Fitton  &  W.  Christopher  Draper,  Jr.;  Sharon  B.  Parente  &  John  W.  Risner;  The  Jefferson  Trust;  Robert  Atkinson;  Dean’s  discretionary  gifts