spc, lpc, pi, coliform - home | university of wisconsin ... · pdf filethe big four spc, lpc,...
TRANSCRIPT
![Page 1: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/1.jpg)
The Big Four SPC, LPC, PI, Coliform
Scott Rankin, Ph.D. University of Wisconsin-Madison
Food Science
Keith Engel WestfaliaSurge, Inc.
Farm Technologies Division
![Page 2: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/2.jpg)
Significance
Regulatory standard Impact on pasteurized product Premium programs Troubleshooting
![Page 3: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/3.jpg)
In general…
Many procedural variations, highly defined practices Method basis: anything present will grow, if it can Individual cells (clusters, chains) each form a Colony
Forming Unit (CFU) CFUs are counted
25-250, 30-300, or? Dilution math is done, e.g. 1:10 dilution, 50 colonies = 500 CFU/mL Might be expressed as log#, e.g. 1000 = 3
Precision – two SDs Can be statistically complex
![Page 4: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/4.jpg)
A plate to be counted
http://water.me.vccs.edu/courses/env108/lessons.htm
![Page 5: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/5.jpg)
One, two, three…uh…?
![Page 6: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/6.jpg)
Now what?
![Page 7: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/7.jpg)
Remember heterotrophic
![Page 8: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/8.jpg)
Petrifilm
![Page 9: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/9.jpg)
SPC Very common, referenced—NCIMS, PMO Standard Plate Count Also called:
APC (Aerobic plate count) HPC (Heterotrophic plate count)
Plate loop count Spiral plate count BactoScan FC Pour plate vs Petrifilm
![Page 10: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/10.jpg)
SPC
Growth conditions SMA (Standard Methods Agar)
Casein digest Yeast extract Sugar (glu)
Aerobic 32°C ± 1; 48 hrs ± 3
![Page 11: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/11.jpg)
LPC Lab Pasteurized Count Same as SPC with one exception:
Milk is first heated to 145°F for 30 min Kills most vegetative cells Activates some spores (from where?) Doesn’t kill “thermoduric” microbes What survives pasteurization?
In cheese In UHT milk Shelf life of fluid milk
![Page 12: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/12.jpg)
Can be a big “Swissue”
![Page 13: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/13.jpg)
PI
Preliminary Incubation count Same as SPC with one exception:
Incubate milk at 55°F for 18 hrs, then plate Growth of cold-lovers (from where?) Compared to standard SPC#
![Page 14: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/14.jpg)
Coliform Count Think “colon” Manure, environment
Aerobic, facultative anaerobic; gram-, nonspore-forming rods
Ferment lactose = acid and gas 32 or 35°F w/in 48 hrs
Escherichia, Enterobacter, Klebsiella
![Page 15: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/15.jpg)
Coliform Count
What’s for dinner? Yeast extract Gelatin digest Lactose (gas)
Neutral red (red)
Bile salts mixture Crystal violet (caning treatment) Salt
![Page 16: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/16.jpg)
Coliform Count
http://water.me.vccs.edu/courses/env108/lessons.htm
![Page 17: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/17.jpg)
Coliform Count
3m.com
![Page 18: SPC, LPC, PI, Coliform - Home | University of Wisconsin ... · PDF fileThe Big Four SPC, LPC, PI, Coliform Scott Rankin, Ph.D. University of Wisconsin-Madison Food Science Keith Engel](https://reader031.vdocuments.us/reader031/viewer/2022030422/5aaa4a4c7f8b9a81188dc978/html5/thumbnails/18.jpg)
The Big Four SPC, LPC, PI, Coliform
Scott Rankin, Ph.D. University of Wisconsin-Madison
Food Science
Keith Engel WestfaliaSurge, Inc.
Farm Technologies Division