solvent free extraction, fractionation, and isolation of ... · interest. hydrocarbon-based solvent...
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[ LCT PREMIER XE ]
Solvent free extraction, fractionation, and isolation of chemical compounds
Superior purity, selectivity, and yield. Lower operating costs.
[ SuPERCRITICaL FLuId EXTRaCTIon SySTEMS ]
Faster and cleaner technology to keep your product safe, uncontaminated, and natural.
The demand for analytical laboratory
techniques is driven by the need
for enhanced information about
a target material, including its
chemical, physical, nutritional,
and pharmaceutical properties.
Increasing concern about the quality, safety, usage,
and disposal of hydrocarbon-based solvents, as
well as stricter regulations on the residual levels
of solvents have all contributed to many industries
switching to SFE technology for the extraction,
fractionation, and isolation of chemical compounds.
Waters® Supercritical Fluid Extraction (SFE) Systems
extract chemical compounds using CO2 in its super-
critical state in place of organic solvents. The result is
[ SuPERCRITICaL FLuId EXTRaCTIon SySTEMS ]
n Environmentally friendly
n Lower operating costs
n Little to no residual solvent
n Processes thermolabile compounds
n Safe
n Scaleable
Faster and cleaner technology to keep your product safe, uncontaminated, and natural.
an extract with little or no residual solvent, superior
purity, selectivity, yield, and lower operating costs
compared to traditional hydrocarbon-based
solvent extraction systems. SFE provides a faster,
safer, and cleaner technology for analysts from the
food, natural product, flavor, fragrance, pharmaceutical,
nutraceutical, polymer, chemical, and cleaning
industries, as well as regulatory agencies and
quality control laboratories.
Green Technology
Waters SFE systems extract chemical compounds using
supercritical CO2 instead of an organic solvent. The
supercritical fluid state occurs when a fluid is above its
critical temperature (Tc) and critical pressure (Pc). This
supercritical state allows CO2 to take on the properties
of a gas (high diffusivity, low surface tension), as well
maintaining the solvent power of a liquid when it is
between the typical gas and liquid states. Manipulating
the temperature and pressure of CO2 alters the solvent
power and allows the material of interest to be
selectively extracted.
The biggest advantage of SFE is that it leaves no traces in
the product. After extraction, the carbon dioxide is either
depressurized and vented, or it can be recycled for further
extraction use. Any residual trace of carbon dioxide in the
product dissipates into the atmosphere within a few hours.
As a tunable solvent, CO2 is non-toxic, non-flammable, and
physiologically compatible.
The biggest advantage of SFE is that you can extract your compound with little or no residual solvent, leaving no traces in your product.
(Above) Tea and coffee are decaffeinated via supercritical fluid extraction. (Center) Most major brewers in the US and Europe use flavors that are extracted from hops using SFE Technology. (Right) More concentrated extracts of St. John’s Wort are obtained through SFE than with conventional technologies.
Ideal tool for a range of applications
SFE is a selective technique that is capable of extracting
compounds from solid maticies to isolate the analyte of
interest. Hydrocarbon-based solvent extraction requires a
distillation/dry down step that may alter the taste, aroma,
and/or chemical composition of the product. Many other
applications have been fully developed and commercialized
incorporating the SFE technique, including:
n Extraction and fractionation of edible fats and oils
n Purification of solid matrices
n Separation of tocopherols and other antioxidants
n Cleanup of herbal medicines and food products from pesticides and herbicides
n Detoxification of shellfish
n Concentration of fermentation broth, fruit juices, etc.
n Flavors, spice extracts, herbs, decaffeination
n Fragrances
n Natural food colors
n Desolvation within tablets
n Dietary supplements, such as St. John’s Wort, saw palmetto, kava-kava, ginger, garlic, and ginseng
n Production of denicotined tobacco
n Production of active ingredients from herbal plants for avoiding thermo or chemical degradation
n Active compounds of all kinds, including steroids and polymer/monomer separation
n Precision parts cleaning
[ Supercritical Fluid extraction SyStemS ]
BLEED VALVE
DRAIN VALVE
ON/OFF VALVE
GAUGE
GAUGE
AUTOMATEDBPR
MANUALBPR
FLOW METER
MIXER
COOLINGHEAT
EXCHANGER
HEAT JACKET EXT RACTIONVESSEL
HEATEXCHANGER
COOLING BATH
CO2 PUMP
CO SOLVENT PUMP
VENT
CO-SOLVENTRESERVOIR
CO2 SUPPLY IN
FRACTIONCOLLECTOR
HEAT JACKET
By manipulating the pressure and temperature, CO2 can
selectively extract the desired material. The sample is
placed in an extraction vessel and pressurized with CO2
and a small percentage of co-solvent to extract the
compound of interest. This compound is then transferred
from the extraction vessel to a fraction collector. The
automated Back Pressure Regulator (BPR), located between
these vessels, allows for controlled depressurization of
the compounds of interest and the CO2. After exiting the
automated BPR, the system pressure is reduced to near
atmospheric conditions, causing the CO2 to lose its
solvating power. The extracted material precipitates out
of the solution into the fraction collector. The condensed
CO2, now a gas, is sent to vent, or it can be recycled.
Extract compounds without altering the taste, aroma, or chemical composition of your product
Waters SFE Systems include a PC with custom software
for instrument control and data management. Regardless
of your native language, the comprehensive, yet simple
icon-based interface is easy to use. The user controls the
pressure, temperature, flow rates, as well as data logging,
data mining, and the ability to write scripts
tailored to your application.
How
Major system components: High pressure CO
2 pump
Known for quality, Waters’ dual-piston CO2
pumps are used by academic and corporate
research scientists in over 40 countries.
This pulseless flow pump requires no air,
operates quietly, and can be used in a
pressure-control or flow-control mode.
Saftey features include mechanical and
electrical ovepressure protection.
High pressure vessel
The finger-tight high pressure vessel
is designed for simple opening and
closing, and even distribution of the fluid
contacting the sample. A threaded cap
with a spring-loaded seal enhances safety
and enables effortles sample loading and
unloading. Waters’ unique stainless steel
is 60% stronger than traditional 304 and
316 stainless steel, which minimizes heat
up and cool down times All vessels are
designed per the ASME pressure code and
CE code if applicable.
Automated Back Pressure Regulator (BPR)
Our state-of-the-art BPR features a
pressure sensor for closed-loop feedback
control of the back pressure, and built-in
heating to prevent freezing.
Easy-to-use software tailored to your application
[ SuPERCRITICaL FLuId EXTRaCTIon SySTEMS ]
Custom software provides easy-to-use instrument control and data management.
High pressure collection
A Waters cyclonic-style collection separator can collect
particles or liquids from gases or liquids. Collection starts
with fluid being introduced at high velocity into the fraction
collector, where centrifugal forces press heavier particles
against the inner wall of the separator, while the lighter
particles exit through the tapered bottom of the fraction
collector. The self-sealing, finger-tight cap is easy to
open and close.
Benchtop to Cart- Mounted Systems Ranging from 100 CC to 2 X 5000 CC
Waters’ benchtop to cart-mounted
systems are pre-assembled and
ready to install. Customer training
courses are available on-site, or at
our training facilities in the US or UK.
n SFE 100
n SFE 500
n SFE 1000
n SFE 2000
n SFE 5000
n SFE 2 X 5 LF
System options include:
n CO2 Flow Meter
n Multiple Extraction Vessels
n Multiple Fraction Collectors
n Recycle System
n Counter Current Column
SFE 1000 System.
Waters, is a registered trademark of Waters Corporation. The Science of What’s Possible is a trademark of Waters Corporation. All other trademarks are the property of their respective owners.
©2010 Waters Corporation. Printed in the U.S.A.October 2010 720003629EN AO-CP
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