SOLAμ for Twenty-fold Sample Concentration Prior to Analysis Jon Bardsley and Ken Meadows Thermo Fisher Scientific, Runcorn, UK

2 SOLAμ for Twenty-fold Sample Concentration Prior to Analysis

© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others.

SOLAµ for Twenty-fold Sample Concentration Prior to AnalysisJon Bardsley, Ken Meadows; Thermo Fisher Scientific, Runcorn, UK

PO20950_E 04/14S

Introduction

Despite advances in analytical detection technology achieving required limits of sensitivity, whilst maintaining reproducibility, can still be an issue for many bioanalyticallaboratories.

Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre-concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non-specific binding.

By using Thermo Scientific™ SOLAµ™ a twenty-fold concentration of sample can be achieved without the need for a lengthy dry-down step, increasing sensitivity without compromising assay performance.

FIGURE 1. Summary of workflow required to achieve a twenty-fold increase in sample concentration prior to analysis without altering the work-flow or compromising assay performance.

Low, mid and high QC samples were prepared at concentrations of 400, 20000 and 30000 pg/mL respectively. Good levels of accuracy at all QC levels were observed. Assay is reproducible for replicate extractions (n=18) at both high and low QC levels.

Analyte recovery was shown to be greater than 89.9% by comparison to post extraction fortified blank samples. Post extraction fortified blank samples were also compared against pure reference standards to demonstrate matrix effects which were calculated at less than 9% at both high and Low QC levels.

DiscussionThis poster demonstrates the advantages of SOLAµ for sample concentration prior to analysis while maintaining high levels of precision, accuracy, recovery and sample cleanliness.

By loading a sample volume of 500 µL and eluting in a volume of 25 µL it is possible to decrease the lower limit of quantitation by a factor of twenty without the need for lengthy evaporation procedures that may compromise sample integrity.

SOLAμ provides reproducibility, robustness and ease of use at low elution volumes by utilizing the revolutionary Thermo Scientific™ SOLA™ , Solid Phase Extraction (SPE) technology. This removes the need for frits delivering a robust, reproducible format which ensures highly consistent results at low elution volumes.

Niflumic AcidY = 0.00375684+5.93882e-005*X R^2 = 0.9975 W: 1/X^2

0 5000 10000 15000 20000 25000 30000 35000 400000.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3

2.4

2.5

2.6

Area

Rat

io

By loading 500 µL of sample onto the SOLAµ plate and eluting in a total of 25 µL a twenty-fold concentration of the analyte was achieved. The results demonstrate that even with low elution volume high levels of accuracy, precision, recovery and sample cleanliness were achieved.

SOLAµ allows users to pre-concentrate the sample up to 20-times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.

FIGURE 2. Linear calibration line for Niflumic acid from 40 to 40000 pg/mL

TABLE 1. Assay accuracy and precision data

FIGURE 3. Twenty-fold increase in response achieved by using SOLAµ plate for sample concentration

TABLE 2. Matrix effects results calculated by comparing fortified blank samples against pure reference standards

TABLE 3. Assay recovery results calculated by comparing extracted samples against fortified blank samples

SOLAμ delivers: • Lower sample failures due to high reproducibility at low elution volumes

• Increased sensitivity due to lower elution volumes

• The ability to process samples which are limited in volume

• Improved stability of bio-molecules by reduction of adsorption and solvation issues

Results

The assay gave a linear dynamic range from 40 to 40000 pg/mL with an r2 coefficient of 0.998. The chromatography for the limit of quantitation sample at 40 pg/mL is significantly above the acceptable signal to noise limit.

3Thermo Scientific Poster Note • PN20950_HPLC_2014_E_05/14S

© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others.

SOLAµ for Twenty-fold Sample Concentration Prior to AnalysisJon Bardsley, Ken Meadows; Thermo Fisher Scientific, Runcorn, UK

PO20950_E 04/14S

Introduction

Despite advances in analytical detection technology achieving required limits of sensitivity, whilst maintaining reproducibility, can still be an issue for many bioanalyticallaboratories.

Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre-concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non-specific binding.

By using Thermo Scientific™ SOLAµ™ a twenty-fold concentration of sample can be achieved without the need for a lengthy dry-down step, increasing sensitivity without compromising assay performance.

FIGURE 1. Summary of workflow required to achieve a twenty-fold increase in sample concentration prior to analysis without altering the work-flow or compromising assay performance.

Low, mid and high QC samples were prepared at concentrations of 400, 20000 and 30000 pg/mL respectively. Good levels of accuracy at all QC levels were observed. Assay is reproducible for replicate extractions (n=18) at both high and low QC levels.

Analyte recovery was shown to be greater than 89.9% by comparison to post extraction fortified blank samples. Post extraction fortified blank samples were also compared against pure reference standards to demonstrate matrix effects which were calculated at less than 9% at both high and Low QC levels.

DiscussionThis poster demonstrates the advantages of SOLAµ for sample concentration prior to analysis while maintaining high levels of precision, accuracy, recovery and sample cleanliness.

By loading a sample volume of 500 µL and eluting in a volume of 25 µL it is possible to decrease the lower limit of quantitation by a factor of twenty without the need for lengthy evaporation procedures that may compromise sample integrity.

SOLAμ provides reproducibility, robustness and ease of use at low elution volumes by utilizing the revolutionary Thermo Scientific™ SOLA™ , Solid Phase Extraction (SPE) technology. This removes the need for frits delivering a robust, reproducible format which ensures highly consistent results at low elution volumes.

Niflumic AcidY = 0.00375684+5.93882e-005*X R^2 = 0.9975 W: 1/X^2

0 5000 10000 15000 20000 25000 30000 35000 400000.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3

2.4

2.5

2.6

Area

Rat

io

By loading 500 µL of sample onto the SOLAµ plate and eluting in a total of 25 µL a twenty-fold concentration of the analyte was achieved. The results demonstrate that even with low elution volume high levels of accuracy, precision, recovery and sample cleanliness were achieved.

SOLAµ allows users to pre-concentrate the sample up to 20-times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.

FIGURE 2. Linear calibration line for Niflumic acid from 40 to 40000 pg/mL

TABLE 1. Assay accuracy and precision data

FIGURE 3. Twenty-fold increase in response achieved by using SOLAµ plate for sample concentration

TABLE 2. Matrix effects results calculated by comparing fortified blank samples against pure reference standards

TABLE 3. Assay recovery results calculated by comparing extracted samples against fortified blank samples

SOLAμ delivers: • Lower sample failures due to high reproducibility at low elution volumes

• Increased sensitivity due to lower elution volumes

• The ability to process samples which are limited in volume

• Improved stability of bio-molecules by reduction of adsorption and solvation issues

Results

The assay gave a linear dynamic range from 40 to 40000 pg/mL with an r2 coefficient of 0.998. The chromatography for the limit of quantitation sample at 40 pg/mL is significantly above the acceptable signal to noise limit.

4 SOLAμ for Twenty-fold Sample Concentration Prior to Analysis

© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others.

SOLAµ for Twenty-fold Sample Concentration Prior to AnalysisJon Bardsley, Ken Meadows; Thermo Fisher Scientific, Runcorn, UK

PO20950_E 04/14S

Introduction

Despite advances in analytical detection technology achieving required limits of sensitivity, whilst maintaining reproducibility, can still be an issue for many bioanalyticallaboratories.

Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre-concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non-specific binding.

By using Thermo Scientific™ SOLAµ™ a twenty-fold concentration of sample can be achieved without the need for a lengthy dry-down step, increasing sensitivity without compromising assay performance.

FIGURE 1. Summary of workflow required to achieve a twenty-fold increase in sample concentration prior to analysis without altering the work-flow or compromising assay performance.

Low, mid and high QC samples were prepared at concentrations of 400, 20000 and 30000 pg/mL respectively. Good levels of accuracy at all QC levels were observed. Assay is reproducible for replicate extractions (n=18) at both high and low QC levels.

Analyte recovery was shown to be greater than 89.9% by comparison to post extraction fortified blank samples. Post extraction fortified blank samples were also compared against pure reference standards to demonstrate matrix effects which were calculated at less than 9% at both high and Low QC levels.

DiscussionThis poster demonstrates the advantages of SOLAµ for sample concentration prior to analysis while maintaining high levels of precision, accuracy, recovery and sample cleanliness.

By loading a sample volume of 500 µL and eluting in a volume of 25 µL it is possible to decrease the lower limit of quantitation by a factor of twenty without the need for lengthy evaporation procedures that may compromise sample integrity.

SOLAμ provides reproducibility, robustness and ease of use at low elution volumes by utilizing the revolutionary Thermo Scientific™ SOLA™ , Solid Phase Extraction (SPE) technology. This removes the need for frits delivering a robust, reproducible format which ensures highly consistent results at low elution volumes.

Niflumic AcidY = 0.00375684+5.93882e-005*X R^2 = 0.9975 W: 1/X^2

0 5000 10000 15000 20000 25000 30000 35000 400000.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3

2.4

2.5

2.6

Area

Rat

io

By loading 500 µL of sample onto the SOLAµ plate and eluting in a total of 25 µL a twenty-fold concentration of the analyte was achieved. The results demonstrate that even with low elution volume high levels of accuracy, precision, recovery and sample cleanliness were achieved.

SOLAµ allows users to pre-concentrate the sample up to 20-times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.

FIGURE 2. Linear calibration line for Niflumic acid from 40 to 40000 pg/mL

TABLE 1. Assay accuracy and precision data

FIGURE 3. Twenty-fold increase in response achieved by using SOLAµ plate for sample concentration

TABLE 2. Matrix effects results calculated by comparing fortified blank samples against pure reference standards

TABLE 3. Assay recovery results calculated by comparing extracted samples against fortified blank samples

SOLAμ delivers: • Lower sample failures due to high reproducibility at low elution volumes

• Increased sensitivity due to lower elution volumes

• The ability to process samples which are limited in volume

• Improved stability of bio-molecules by reduction of adsorption and solvation issues

Results

The assay gave a linear dynamic range from 40 to 40000 pg/mL with an r2 coefficient of 0.998. The chromatography for the limit of quantitation sample at 40 pg/mL is significantly above the acceptable signal to noise limit.

5Thermo Scientific Poster Note • PN20950_HPLC_2014_E_05/14S

© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others.

SOLAµ for Twenty-fold Sample Concentration Prior to AnalysisJon Bardsley, Ken Meadows; Thermo Fisher Scientific, Runcorn, UK

PO20950_E 04/14S

Introduction

Despite advances in analytical detection technology achieving required limits of sensitivity, whilst maintaining reproducibility, can still be an issue for many bioanalyticallaboratories.

Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre-concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non-specific binding.

By using Thermo Scientific™ SOLAµ™ a twenty-fold concentration of sample can be achieved without the need for a lengthy dry-down step, increasing sensitivity without compromising assay performance.

FIGURE 1. Summary of workflow required to achieve a twenty-fold increase in sample concentration prior to analysis without altering the work-flow or compromising assay performance.

Low, mid and high QC samples were prepared at concentrations of 400, 20000 and 30000 pg/mL respectively. Good levels of accuracy at all QC levels were observed. Assay is reproducible for replicate extractions (n=18) at both high and low QC levels.

Analyte recovery was shown to be greater than 89.9% by comparison to post extraction fortified blank samples. Post extraction fortified blank samples were also compared against pure reference standards to demonstrate matrix effects which were calculated at less than 9% at both high and Low QC levels.

DiscussionThis poster demonstrates the advantages of SOLAµ for sample concentration prior to analysis while maintaining high levels of precision, accuracy, recovery and sample cleanliness.

By loading a sample volume of 500 µL and eluting in a volume of 25 µL it is possible to decrease the lower limit of quantitation by a factor of twenty without the need for lengthy evaporation procedures that may compromise sample integrity.

SOLAμ provides reproducibility, robustness and ease of use at low elution volumes by utilizing the revolutionary Thermo Scientific™ SOLA™ , Solid Phase Extraction (SPE) technology. This removes the need for frits delivering a robust, reproducible format which ensures highly consistent results at low elution volumes.

Niflumic AcidY = 0.00375684+5.93882e-005*X R^2 = 0.9975 W: 1/X^2

0 5000 10000 15000 20000 25000 30000 35000 400000.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3

2.4

2.5

2.6

Area

Rat

io

By loading 500 µL of sample onto the SOLAµ plate and eluting in a total of 25 µL a twenty-fold concentration of the analyte was achieved. The results demonstrate that even with low elution volume high levels of accuracy, precision, recovery and sample cleanliness were achieved.

SOLAµ allows users to pre-concentrate the sample up to 20-times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.

FIGURE 2. Linear calibration line for Niflumic acid from 40 to 40000 pg/mL

TABLE 1. Assay accuracy and precision data

FIGURE 3. Twenty-fold increase in response achieved by using SOLAµ plate for sample concentration

TABLE 2. Matrix effects results calculated by comparing fortified blank samples against pure reference standards

TABLE 3. Assay recovery results calculated by comparing extracted samples against fortified blank samples

SOLAμ delivers: • Lower sample failures due to high reproducibility at low elution volumes

• Increased sensitivity due to lower elution volumes

• The ability to process samples which are limited in volume

• Improved stability of bio-molecules by reduction of adsorption and solvation issues

Results

The assay gave a linear dynamic range from 40 to 40000 pg/mL with an r2 coefficient of 0.998. The chromatography for the limit of quantitation sample at 40 pg/mL is significantly above the acceptable signal to noise limit.

6 SOLAμ for Twenty-fold Sample Concentration Prior to Analysis

© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others.

SOLAµ for Twenty-fold Sample Concentration Prior to AnalysisJon Bardsley, Ken Meadows; Thermo Fisher Scientific, Runcorn, UK

PO20950_E 04/14S

Introduction

Despite advances in analytical detection technology achieving required limits of sensitivity, whilst maintaining reproducibility, can still be an issue for many bioanalyticallaboratories.

Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre-concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non-specific binding.

By using Thermo Scientific™ SOLAµ™ a twenty-fold concentration of sample can be achieved without the need for a lengthy dry-down step, increasing sensitivity without compromising assay performance.

FIGURE 1. Summary of workflow required to achieve a twenty-fold increase in sample concentration prior to analysis without altering the work-flow or compromising assay performance.

Low, mid and high QC samples were prepared at concentrations of 400, 20000 and 30000 pg/mL respectively. Good levels of accuracy at all QC levels were observed. Assay is reproducible for replicate extractions (n=18) at both high and low QC levels.

Analyte recovery was shown to be greater than 89.9% by comparison to post extraction fortified blank samples. Post extraction fortified blank samples were also compared against pure reference standards to demonstrate matrix effects which were calculated at less than 9% at both high and Low QC levels.

DiscussionThis poster demonstrates the advantages of SOLAµ for sample concentration prior to analysis while maintaining high levels of precision, accuracy, recovery and sample cleanliness.

By loading a sample volume of 500 µL and eluting in a volume of 25 µL it is possible to decrease the lower limit of quantitation by a factor of twenty without the need for lengthy evaporation procedures that may compromise sample integrity.

SOLAμ provides reproducibility, robustness and ease of use at low elution volumes by utilizing the revolutionary Thermo Scientific™ SOLA™ , Solid Phase Extraction (SPE) technology. This removes the need for frits delivering a robust, reproducible format which ensures highly consistent results at low elution volumes.

Niflumic AcidY = 0.00375684+5.93882e-005*X R^2 = 0.9975 W: 1/X^2

0 5000 10000 15000 20000 25000 30000 35000 400000.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3

2.4

2.5

2.6

Area

Rat

io

By loading 500 µL of sample onto the SOLAµ plate and eluting in a total of 25 µL a twenty-fold concentration of the analyte was achieved. The results demonstrate that even with low elution volume high levels of accuracy, precision, recovery and sample cleanliness were achieved.

SOLAµ allows users to pre-concentrate the sample up to 20-times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.

FIGURE 2. Linear calibration line for Niflumic acid from 40 to 40000 pg/mL

TABLE 1. Assay accuracy and precision data

FIGURE 3. Twenty-fold increase in response achieved by using SOLAµ plate for sample concentration

TABLE 2. Matrix effects results calculated by comparing fortified blank samples against pure reference standards

TABLE 3. Assay recovery results calculated by comparing extracted samples against fortified blank samples

SOLAμ delivers: • Lower sample failures due to high reproducibility at low elution volumes

• Increased sensitivity due to lower elution volumes

• The ability to process samples which are limited in volume

• Improved stability of bio-molecules by reduction of adsorption and solvation issues

Results

The assay gave a linear dynamic range from 40 to 40000 pg/mL with an r2 coefficient of 0.998. The chromatography for the limit of quantitation sample at 40 pg/mL is significantly above the acceptable signal to noise limit.

Thermo Fisher Scientific, Sunnyvale, CA USA is ISO 9001:2008 Certified.

www.thermoscientific.com©2014 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. This information is presented as an example of the capabilities of Thermo Fisher Scientific Inc. products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

PN20950_E 05/14S

Africa +43 1 333 50 34 0Australia +61 3 9757 4300Austria +43 810 282 206Belgium +32 53 73 42 41Brazil +55 11 3731 5140Canada +1 800 530 8447China 800 810 5118 (free call domestic)

400 650 5118

Denmark +45 70 23 62 60Europe-Other +43 1 333 50 34 0Finland +358 9 3291 0200France +33 1 60 92 48 00Germany +49 6103 408 1014India +91 22 6742 9494Italy +39 02 950 591

Japan +81 6 6885 1213Korea +82 2 3420 8600Latin America +1 561 688 8700Middle East +43 1 333 50 34 0Netherlands +31 76 579 55 55 New Zealand +64 9 980 6700 Norway +46 8 556 468 00

Russia/CIS +43 1 333 50 34 0Singapore +65 6289 1190Sweden +46 8 556 468 00 Switzerland +41 61 716 77 00Taiwan +886 2 8751 6655UK/Ireland +44 1442 233555USA +1 800 532 4752

© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others.

SOLAµ for Twenty-fold Sample Concentration Prior to AnalysisJon Bardsley, Ken Meadows; Thermo Fisher Scientific, Runcorn, UK

PO20950_E 04/14S

Introduction

Despite advances in analytical detection technology achieving required limits of sensitivity, whilst maintaining reproducibility, can still be an issue for many bioanalyticallaboratories.

Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre-concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non-specific binding.

By using Thermo Scientific™ SOLAµ™ a twenty-fold concentration of sample can be achieved without the need for a lengthy dry-down step, increasing sensitivity without compromising assay performance.

FIGURE 1. Summary of workflow required to achieve a twenty-fold increase in sample concentration prior to analysis without altering the work-flow or compromising assay performance.

Low, mid and high QC samples were prepared at concentrations of 400, 20000 and 30000 pg/mL respectively. Good levels of accuracy at all QC levels were observed. Assay is reproducible for replicate extractions (n=18) at both high and low QC levels.

Analyte recovery was shown to be greater than 89.9% by comparison to post extraction fortified blank samples. Post extraction fortified blank samples were also compared against pure reference standards to demonstrate matrix effects which were calculated at less than 9% at both high and Low QC levels.

DiscussionThis poster demonstrates the advantages of SOLAµ for sample concentration prior to analysis while maintaining high levels of precision, accuracy, recovery and sample cleanliness.

By loading a sample volume of 500 µL and eluting in a volume of 25 µL it is possible to decrease the lower limit of quantitation by a factor of twenty without the need for lengthy evaporation procedures that may compromise sample integrity.

SOLAμ provides reproducibility, robustness and ease of use at low elution volumes by utilizing the revolutionary Thermo Scientific™ SOLA™ , Solid Phase Extraction (SPE) technology. This removes the need for frits delivering a robust, reproducible format which ensures highly consistent results at low elution volumes.

Niflumic AcidY = 0.00375684+5.93882e-005*X R^2 = 0.9975 W: 1/X^2

0 5000 10000 15000 20000 25000 30000 35000 400000.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3

2.4

2.5

2.6

Area

Rat

io

By loading 500 µL of sample onto the SOLAµ plate and eluting in a total of 25 µL a twenty-fold concentration of the analyte was achieved. The results demonstrate that even with low elution volume high levels of accuracy, precision, recovery and sample cleanliness were achieved.

SOLAµ allows users to pre-concentrate the sample up to 20-times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.

FIGURE 2. Linear calibration line for Niflumic acid from 40 to 40000 pg/mL

TABLE 1. Assay accuracy and precision data

FIGURE 3. Twenty-fold increase in response achieved by using SOLAµ plate for sample concentration

TABLE 2. Matrix effects results calculated by comparing fortified blank samples against pure reference standards

TABLE 3. Assay recovery results calculated by comparing extracted samples against fortified blank samples

SOLAμ delivers: • Lower sample failures due to high reproducibility at low elution volumes

• Increased sensitivity due to lower elution volumes

• The ability to process samples which are limited in volume

• Improved stability of bio-molecules by reduction of adsorption and solvation issues

Results

The assay gave a linear dynamic range from 40 to 40000 pg/mL with an r2 coefficient of 0.998. The chromatography for the limit of quantitation sample at 40 pg/mL is significantly above the acceptable signal to noise limit.