SOD Research 5/09

- SOD Spore Surveys & SOD Blitz / Outreach

- Phosphonate Treatment of Tanoaks and CLOs

- Long Term Efficacy of SOD Treatments

- Resistance / Tolerance in Tanoaks

- Transmission of SOD Through Pruning

- Management Planning

SOD Spore MonitoringSOD Spore Monitoring

8 Field Monitoring Sites8 Field Monitoring Sites 16 Buckets / Site16 Buckets / Site 5 5 Leaves / BucketLeaves / Bucket = 640 leaves sampled every 3 weeks= 640 leaves sampled every 3 weeks Starting again in February 08Starting again in February 08

Bay Laurel / Tanoak SOD Spore Survey

Date

Temp (C)

Rain (mm)

SOD Blitz – Disease MonitoringSOD Blitz – Disease Monitoring

SOD Blitz / SOD Treatment Training Sessions

Topical TreatmentTopical TreatmentInjection TreatmentInjection Treatment

Save the Tanoaks ProjectSave the Tanoaks Project

SOD Treatment SitesSOD Treatment SitesLocation: Sites Plot Pairs TreesCarmel Valley 2 6 54

SDSF 2 4 883

LPNF 1 2 36

MPRPD 1 2 46

Homeowners 13 na 107

Long Term Efficacy of SOD Treatments

0

20

40

60

80

100

Time 0 3 Months 6 Months 1 Year 1.5 Year 2 Year 2.5 Year

Agrifos vs. Azomite TreatmentsC

anke

r Siz

e (%

of U

ntre

ated

Pos

itive

Con

trol)

Detached Branch Assay

Novato Mature CLOs

Agrifos & PentraBark

Azomite & Limewash

Long Term Efficacy of SOD Treatments

0

5

10

15

20

25

Large Medium

Tree Size Class

Les

ion

Size

(mm

) PCTopicalInjectNC

Effect of Tree Size on Treatment Efficacy

Tanoak Common Garden Experiment Shadehouse

Tanoak Out Planting – Common Garden Experiment

Tanoak Assays

Tanoak PetiolePlug Assay

UntreatedControl Tree

Phosphonate Treated Tree

Whole Seedling Inoculation

Infected Seedling

00

11

22

33

44

55

66

77

88

99

11 22 33 44 55 66 88 99 1111 1313 1414 1515 1616 1717 1818 1919 2121 2323 2424 2525 5050

FamilyFamily

Mea

n Le

sion

Len

gth,

Apr

09M

ean

Lesi

on L

engt

h, A

pr09

Whole-seedling inoculation

Zoospores are in Direct Contact with

Tree for 24 hrs.

Grafting Wax Creates a Water Tight Seal

Zoospore Inoculator for P. Ramorum

1 cm

Transmission of SOD through pruning:

Zoospore Inoculator

Pruning CLO Branches

► Does tree pruning introduce SOD infection?► Is the timing of cutting a factor?

Test at: 1 week 1 month 2 months 4 months

Grady Ranch Site

Transmission of SOD Through Pruning

01020304050607080

1 Week 1 Month 2 Months 4 Months

Time Post Pruning

Infe

cted

Bra

nche

s (%

)

Grady Ranch11/27/07

Transmission of SOD Through Pruning

Air Sampling for SOD Spores

Steam Baiting for SOD Monitoring