SNAPGENEVIEW RICHLY ANNOTATED SEQUENCE FILES

Manish ThakurSystems Biology and

BioinformaticsM.Sc-1

OUTLINE

INTRODUCTIONWHAT SNAPGENE DO

• VISUALIZE

• ANNOTATE

• PLAN & DOCUMENT

STRATEGIES FOLLOWED OUTPUT CONCLUSIONS

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures. The streamlined interface supports a range of cloning and PCR manipulations.

SnapGene was developed by GSL Biotech Chicago.

First version of SnapGene was 0.2 released on Apr 3, 2008.

Latest version available SnapGene 2.7.1 on Mar 6, 2014.

Buy/Free trial of the software from the SnapGene website www.snapgene.com.

INTRODUCTION

SYSTEM REQUIREMENTS

O.S Windows 8, 7, Vista or XP

Mac OS X 10.7 or later

Memory 1 GB RAM

Hard Disk 250 MB available disk space

Display 1024 x 768 or higher resolution

Written in Visual Studio, C++

• Create a DNA sequence file.

• Browse or print a DNA sequence and its annotations using customizable Map, Sequence, Enzymes, Features, Primers, and History views.

• Search a DNA sequence to match either a DNA query, or a protein translation, or an annotation.

• Open and edit sequence trace files, and align them to a reference sequence.

VISUALIZE

•Automatically annotate common features, or manually annotate coding sequences and other features.

•Design and annotate primers for PCR, sequencing, or mutagenesis.

•Identify open reading frames (ORFs) with a single mouse click.

ANNOTATE

• Simulate restriction cloning and other types of cloning using a rich intuitive interface.

•Simulate conventional or overlap extension PCR.

•Simulate primer-directed mutagenesis.

•Simulate agarose gel electrophoresis.

•Record all of your manipulations in an automatically generated graphical cloning history.

PLAN AND DOCUMENT

What we can do with SnapGene

o Restriction Cloning

o Primers & PCR

o Mutagenesis

o Gibson Assembly

o In-Fusion Cloning

o Gateway Cloning

o TA or GC cloning

Restriction CloningCloning by restriction enzyme digestion and ligation is a simple and easy way of moving a fragment of double stranded DNA from one plasmid to another.

PRIMER DESIGNING & PCR

 Amplify a single copy or a few copies of a piece of DNA.

PCR Machine

Restriction Cloning

SnapGene has a specific purpose and it is highly flexible as far as finding items or groups of similar items. Its applicability is restricted to the field of molecular biology.

I perform restriction cloning ,Primers & PCR strategy under SnapGene

It is an indispensable tool for building plasmid maps of our various recombinant constructs to aid with the cloning process. It is a user-friendly & time saver software for RDT projects.

CONCLUSION

oEvery DNA construct in a lab will be fully documented.

oPublications will include complete annotated sequences and histories of every DNA construct used.

oTime and money will be saved, and biomedical research will move faster.

Vision of the Future with SnapGene