slides examبكتريا عملي
TRANSCRIPT
![Page 1: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/1.jpg)
Diagnostic Microbiology Lab.
By
Dr. Nabil El Aila
Assistant Professor of Molecular Microbiology
Medical Technology Department
Al -Aqsa University
![Page 2: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/2.jpg)
A B
Name of the test: Catalase test
Example A: positive - Staphylococci
Example B: Negative - Streptococci
Principle: 2 H2O2 2 H2O + O2
Catalase
![Page 3: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/3.jpg)
A B
Name of the test: Coagulase test
Example A: Positive - Staph aureus
Example B: Negative - Staph epidermedis
Principle: Fibrinogen in plasma Fibrin clot Coagulase
![Page 4: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/4.jpg)
A B
Name of the test: DNase test
Example A: Positive - Staph aureus
Example B: Negative - Staph epidermedis
Priciple: DNA is insoluble in acid
DNA is hydrolyzed into oligonucleotides by the action of Dnase
Nucleotides soluble in acid (After addition of 1N HCl)
![Page 5: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/5.jpg)
Name of the test: Mannitol salt agar
Example A: Negative - Staph epidermidis
Example B: Positive - Staph aureus
Principle: Staph aureus ferment mannitol (sugar) and phenol red (pH
indicators turns yellow in acidic pH and turns red in alkaline pH).
A B
![Page 6: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/6.jpg)
Name of the test: Novobiocin sensitivity
Example A: Staph epidermidis (Sensitive)
Example B:Staph saprophyticus (Resistant)
A
B
![Page 7: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/7.jpg)
Name of the test: Bacitracin sensitivity
Example A: Strept pyogens (sensitive)
Example B: Strept agalactiae (resistant)
A B
![Page 8: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/8.jpg)
A
B
Name of the test: CAMP test
Example A: Positive - Strept agalactiae (Arrow shaped)
Example B: Negative - Strept pyogens
Principle : Strept agalactiae produce CAMP factor (a diffusible extracelluar
protein) that synergistically acts with the beta-lysin of Staphylococcus aureus
and enhances the lysis of red blood cells.
![Page 9: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/9.jpg)
Name of the test: Optochin sensitivity
Example A: Strept pneumonia (sensitive)
Example B: Strept viridans (resistant)
A B
![Page 10: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/10.jpg)
A B
Name of the test: Bile Esculin test
Example A: Positive - Group D streptococcus (Enterococcus species)
Example B: Negative - Group B streptococcus
Principle: The selective agent bile, inhibits most gram positive bacteria.
Esculin in the medium is hydrolyzed to esculetin and dextrose.
The esculetin reacts with ferric chloride in the media to form a black-brown
color.
![Page 11: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/11.jpg)
B A
Name of the test: Bile Solubility
Example A: Strept mitis (Negative)
Example B: Strept pneumoniae (positive)
Principle: S. pneumoniae is bile soluble whereas all other alpha-hemolytic
streptococci are bile resistant. Sodium deoxycholate (2% in water) will lyse
the pneumococcal cell wall
![Page 12: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/12.jpg)
A B
Name of the test: Pyrrolidonyl arylamidase Test (PYR) Example A: Strept agalactiae (Negative)
Example B: group A streptococci and Enterococcu s species (positive)
Principle: Group A streptococci and Enterococcu s species produce
pyrrolidonyl arylamidase which hydrolyzes the substrate L- pyrrolidonyl -β-
naphthylamide to form β-naphthylamine.
A pink to red color forms when p-dimethylaminocinnam-aldehyde (PYR
reagent) is added to β-naphthylamine.
![Page 13: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/13.jpg)
A B
Tryptophane
amino acids
Tryptophanase Indole + Pyurvic acid + NH3
Kovac’s Reagent
Red color in upper organic layer`
Name of the test: Indole test
Example A: Klebsiella pneumonia (Negative)
Example B: Escherichia coli (Positive)
Indicator: Kovac’s Reagent (4 (p) – dimethylamino benzaldehyde)
Principle:
![Page 14: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/14.jpg)
A B
Name of the test: Methyl red
Example A: Klebsiella pneumonia (Negative)
Example B: Escherichia coli (Positive)
Indicator: Methy red indicator
Principle: follow next slides
![Page 15: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/15.jpg)
A B
Name of the test: Voges Proskauer test
Example A: Escherichia coli (Negative)
Example B: Klebsiella pneumonia (Positive)
Indicator: -naphthol and 40% KOH
Principle: follow next slide
![Page 16: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/16.jpg)
IMViC test
Methyl Red-Voges Proskauer (MR-VP) Tests
Glucose
Acidic pathway
Mixed acids
pH less than 4.4
Methyl Red
indicator
Red color
Principle
MR positive
E. coli
Or Neutral pathway
Acety methyl carbinol
(ACETOIN)
Barrit’s A ( -naphthol)
Barrit;s B (40% KOH)
Pink color VP positive
Klebsiella
![Page 17: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/17.jpg)
Name of the test: Citrate test
Example A: Klebsiella pneumonia (Positive)
Example B: Escherichia coli (Negative)
Indicator: Bromothymol blue
Principle: follow next slide
A B
![Page 18: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/18.jpg)
Principle:
Citrate Na2CO3
Alkaline,↑pH
Bromothymol blue
Simmone’s Citrate media
CO2 + Na + H2O Pyruvate
Positive test
Contains Citrate as a sole of C source
IMViC: CITRATE TEST
Dr. Nabil El Aila Diagnostic Microbiology
![Page 19: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/19.jpg)
Name of the test: Urea test
Example A: Proteus miribalis (Positive)
Example B: Escherichia coli (Negative)
Indicator: Phenol red
Principle:
A B
Urea Urease
CO2 + NH3 H2O
NH4 OH ↑ in pH
Phenol Red
Pink
Positive test
![Page 20: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/20.jpg)
A B
Name of the test: Motility test
Example A: Escherichia coli (Negative)
Example B: Proteus miribalis (Positive)
![Page 21: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/21.jpg)
A B
Name of the test: H2S production
Example A: Escherichia coli (Negative)
Example B : Proteus miribalis (Positive)
Principle:
Bacteria use enzyme cysteine desulfurase to hydrolyze the amino acid
cysteine, forming hydrogen sulfide as end-product.
The hydrogen sulfide will combine with the iron salt to form a visible black
ferric sulfide (FeS) in the tube
![Page 22: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/22.jpg)
A B Name of the test: Triple sugar Iron
Example A: K/A - No H2S – no gas production
Glucose fermenter - Shigella species
Example B: A/A - No H2S – gas production
All three sugars fermented – Escherichia coli
![Page 23: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/23.jpg)
A B Name of the test: Triple sugar Iron
Example A: A/A - H2S positive –gas production
Glucose fermenter - Proteus species
Example B: K/A - H2S positive – gas production
All three sugars fermented – Salmonella species
![Page 24: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/24.jpg)
A B
Name of the test: Triple sugar Iron
Example A: K/K – No H2S – No gas production
No action on sugars - Pseudomonas species
Example B: K/A – No H2S – No gas production
Glucose fermenter - Shigella species
![Page 25: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/25.jpg)
Name of the test: Lysine Decarboxylase Agar
Example A: Positive decarboxylation (butt), negative deamination (slant)
: Salmonella species
Example B: Negative decarboxylation (butt), Positive deamination (slant)
: Proteus species
A B
![Page 26: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/26.jpg)
A B
Name of the test: Lysine Decarboxylase Agar
Example A: Positive decarboxylation (butt), negative deamination (slant)
: Salmonella species
Example B: Negative decarboxylation (butt), Negative deamination (slant)
: Citrobacter species
![Page 27: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/27.jpg)
Name of the test: Oxidase test
Example A: Positive - Pseudomonas species
Example B: Negative - Proteus species
Princile: follow next slide
A B
![Page 28: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/28.jpg)
Oxidase Test: Principal
Oxidase Reagent
Cytochrome Oxidase
Indophenol
Play role in aerobic respiration Pseudomonas
Vibrio
Alternative substrate for Cytochrome
Tetramethyl-P-Pheneylenediamine
Colorless
Purple color
Oxidize the reagent from colorless to purple color
![Page 29: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/29.jpg)
A B
Name of the test: Oxidation Fermentation test
Example A: Oxidation negative (Open tube remain green)
Example B: Fermantation negative ( Covered tube remain green)
: Oxidation negative/ Fermentation negative (Alcaligenes faecalis)
![Page 30: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/30.jpg)
A B
Name of the test: Oxidation Fermentation test
Example A: Oxidation positive (Open tube turns yellow)
Example B: Fermantation negative ( Covered tube remain green)
: Oxidation positive/ Fermentation negative (Pseudomonas species)
![Page 31: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/31.jpg)
A B
Name of the test: Oxidation Fermentation test
Example A: Oxidation positive (Open tube turns yellow)
Example B: Fermantation positive( Covered tube turns yellow)
: Oxidation positive/ Fermentation positive (Enterobacteriacea)
![Page 32: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/32.jpg)
Name of the test: Gelatin Liquification test
Example A: Negative (Proteus species)
Example B: Positive ( Pseudomonas species)
Principle :
Nutrient gelatin
Protein/Polypeptides
Solid
Gelatinase
Incubation at 37/overnight
Nutrient gelatin
Amino acids
Liquid at > 25 C
Gelatinase hydrolyze the protein to aminoacids
Pseudomonas
A
B
![Page 33: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/33.jpg)
A
B
C
D
Name of the test: Nitrate reduction test
Example A: Negative nitrate reduction - no change with alpha naphthol and sulf.acid
Example B: Positive nitrate reduction – turns red with alpha naphthol and sulf.acid
sulf.acid
Example C: Positive nitrate reduction . No red color after addition of zinc dust (
Nitrate reduced into nitrite and further reduction to Nitrogen.
Example D: Negative nitrate reduction red color after addition of zinc dust Nitrate
unreduced
Principle : follow next slide
![Page 34: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/34.jpg)
Nitrate Reductase Test: Principal
Nitrate (NO3)
Nitrate reductase Nitrite (NO2)
α-naphthylamine Sulfanilic acid
Red diazonium salt
If no red color!
Further reduction
Nitrogen gas N2
Add zinc dust (reducing agent)
Nitrate Reductase
No red color after addition of zinc dust
( Nitrate reduced into nitrite and further
reduction to Nitrogen (Positive nitrate
reduction )
or red color after addition of zinc dust
Nitrate unreduced (Negative nitrate
reduction )
![Page 35: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/35.jpg)
Name of the test: Satellitism
Principle:
- A lawn of test bacteria is plated onto a fresh blood agar plate. This provides
a supply of haem.
- An inoculum of Staphylococcus aureus, which can provide NAD, is placed
on the plate and the culture is incubated.
- Haemophili can be seen growing larger near to the staphylococcal colony,
where the supply of NAD is greatest.
![Page 36: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/36.jpg)
Name of the test: Swarming
Example: Proteus species move very actively by means of peritrichous
flagella, resulting in "swarming" on solid medium
![Page 37: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/37.jpg)
Name of the test: Exopigment production on Muller Hinton agar
Example: pseudomonas species produces exopigments in culture eg
pyocyanin, fluorescein, pyorubrin, pyoverdin, and pyomelanin
![Page 38: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/38.jpg)
Bacteria grow in the presence of X factor
Example: Haemophillus aphrophilus
![Page 39: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/39.jpg)
Bacteria grow in the presence of V factor
Example: Haemophillus parainfluenza
![Page 40: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/40.jpg)
A
B
A: Vaginal saline preperation: normal epithelial cells B: Vaginal saline preperation: Clue cells
![Page 41: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/41.jpg)
Gram variable bacteria
(Gram negative and positive coccobacilli)
Gardnerella vaginallis
![Page 42: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/42.jpg)
Name of test: Motility test listeria
A: Non motile
B: umbrella shape indicative of motility
A B
![Page 43: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/43.jpg)
A: Dark field microscopy
B: Fluorescence microscopy antibody staining
Dr. Nabil El Aila Diagnostic Microbiology
A B
![Page 44: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/44.jpg)
Grram positiv cocci in Clusters
Example: Staph aureus
Dr. Nabil El Aila General Microbiology
![Page 45: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/45.jpg)
Grram positiv cocci in Tetrads
Example: Micrococcus species
Dr. Nabil El Aila General Microbiology
![Page 46: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/46.jpg)
Grram positiv cocci in Chains
Example: Streptococcus pyogens
Dr. Nabil El Aila General Microbiology
![Page 47: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/47.jpg)
Grram positiv cocci in pairs
Example: Streptococcus pneumoniae
Dr. Nabil El Aila General Microbiology
![Page 48: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/48.jpg)
Gram negative coccobacilli
Example: Haemophilus influenza
Dr. Nabil El Aila General Microbiology
![Page 49: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/49.jpg)
Grram positiv bacilli (spore forming)
Example: Bacillus anthracis
Dr. Nabil El Aila General Microbiology
![Page 50: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/50.jpg)
Gram positiv bacilli (non spore forming)
Example: Lactobacillus species
Dr. Nabil El Aila General Microbiology
![Page 51: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/51.jpg)
Gram positiv bacilli (non spore forming)
(Chines letter)
Example: Corynebacterium diphtheria
Dr. Nabil El Aila General Microbiology
![Page 52: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/52.jpg)
Gram Negative diplococci
Example: Neisseria gonorrhoeae
Neisseria meningitidis
Dr. Nabil El Aila General Microbiology
![Page 53: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/53.jpg)
Gram Negative bacilli
Example: Escherichia coli
Dr. Nabil El Aila General Microbiology
![Page 54: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/54.jpg)
Gram Negative curved rods (Comma shaped)
Example: Vibrio cholera
Dr. Nabil El Aila General Microbiology
![Page 55: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/55.jpg)
Acid fast bacilli
Example: Mycobacterium tuberculosis
Dr. Nabil El Aila General Microbiology
![Page 56: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/56.jpg)
Gram Negative spiral rods
Example: Campylobacter species
Dr. Nabil El Aila General Microbiology
![Page 57: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/57.jpg)
Spirochates
Example: Treponema pallidum
![Page 58: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/58.jpg)
Type of culture media: Basic Media
Example: Nutrient agar
Microbe: Staph epidermidis
![Page 59: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/59.jpg)
Type of culture media: Enriched Media
Example: Blood agar (golden yellow)
Microbe: Staph aureus
Dr. Nabil El Aila General Microbiology
![Page 60: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/60.jpg)
Type of culture media: Enriched Media
Example: Blood agar (white colonies)
Microbe: Staph epidermidis
Staph saprophyticus Dr. Nabil El Aila
General Microbiology
![Page 61: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/61.jpg)
Type of culture media: Enriched Media
Example: Blood agar (Alpha haemolysis)
Microbe: Strept pneumoniae
![Page 62: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/62.jpg)
Type of culture media: Enriched Media
Example: Blood agar (Beta haemolysis)
Microbe: Strept pyogens
![Page 63: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/63.jpg)
Type of culture media: Enriched Media
Example: Blood agar (Gamma haemolysis)
Microbe: Enterococcus faecalis
![Page 64: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/64.jpg)
Type of culture media: Enriched Media
Example: Chocalate agar
Microbe: Haemophilus influenza
Dr. Nabil El Aila
General Microbiology
![Page 65: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/65.jpg)
Type of culture media: Differential Media
Example: MacConkey agar
Microbe: Escherichia coli
![Page 66: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/66.jpg)
Type of culture media: Differential Media
Example: MacConkey agar
Microbe: Klebsiella pneumoniae
![Page 67: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/67.jpg)
Type of culture media: Differential Media
Example: TCBS (Thiosulfate citrate bile salts sucrose agar)
Microbe: Vibrio cholera
Dr. Nabil El Aila General Microbiology
![Page 68: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/68.jpg)
Type of culture media: Differential Media
Example: CLED (Cystine-Lactose-Electrolyte-Deficient Agar )
Microbe: Escherichia coli
Dr. Nabil El Aila General Microbiology
![Page 69: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/69.jpg)
Type of culture media: Selective Media
Example: Salmonella shigella agar
Microbe: Salmonella typhi
![Page 70: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/70.jpg)
Type of culture media: Selective Media
Example: Xylose lysine Deoxycholate
Microbe: Salmonella typhi
![Page 71: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/71.jpg)
Type of culture media: Selective Media
Example: Hecktoen Enteric
Microbe: Salmonella typhi
Dr. Nabil El Aila General Microbiology
![Page 72: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/72.jpg)
Type of culture media: Selective Media
Example: Mannitol salt agar
Microbe: Staph aureus
Dr. Nabil El Aila General Microbiology
![Page 73: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/73.jpg)
Type of culture media: Selective Media
Example: Lewenstein Jensen Medium
Microbe: Mycobacterium tuberculosis
![Page 74: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/74.jpg)
Muller Hinton Agar
Used for antibiotic susceptibility testing
![Page 75: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/75.jpg)
Dr. Nabil El Aila General Microbiology
Capsule stain
![Page 76: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/76.jpg)
Dr. Nabil El Aila General Microbiology
Negative stain
![Page 77: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/77.jpg)
Dr. Nabil El Aila General Microbiology
Spore stain
![Page 78: Slides examبكتريا عملي](https://reader034.vdocuments.us/reader034/viewer/2022042701/55a732eb1a28ab80028b47cb/html5/thumbnails/78.jpg)
Dr. Nabil El Aila General Microbiology
Flagellar stain