shredder data brochure.penultimatel.ls.033011 vg

8/3/2019 Shredder Data Brochure.penultimatel.ls.033011 VG

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Pressure BioSciences, Inc.

Gentle, Rapid, and Safe Disruption of Tissues and Organisms for

the Extraction of DNA, RNA, Protein, Mitochondria, Lipid, and Small Molecules

The PCT Shredder or TheSHREDDER SG3

PBI Shredder Systems

Discovery Starts with Sample Preparation

Barocycler, PULSETubes, The PCT Shredder, TheSHREDDER SG3PCT Shredder PULSE Tubes and Shredder Tubes

are trademarks of Pressure BioSciences, Inc.


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PBI Shredder Systems are low shear mechanical devices for gentle, rapid, and safedisruption of tissues and organisms. Both The PCT Shredderand TheSHREDDER SG3 canprovide effective extraction of DNA, RNA, proteins, mitochondria, lipids and small moleculesfrom tissues and organisms, when used with Shredder Tubes and various buffers.

Both systems use a variety of Shredder Tubes to directly and rapidly grind solid samples

providing easy handling and reducing sample contamination. And, although not required forall extractions, additional extraction efficiency can be achieved when this process is combinedwith pressure cycling technology (PCT).

The PCT Shredder

Features and Benefits of The PCT Shredder

Portable Sturdy, Stainless Steel Construction

Cordless

Compact and Lightweight driver

PBI SHREDDER SYSTEMS

Features and Benefitsof TheSHREDDER SG3

Long Lasting Lithium Rechargeable Batteries

Heavy Duty Robust Driver

Three Position Lever for Setting Reproducible Force

TheSHREDDER SG3


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SHREDDER TUBES

Tube Type Color Metal Disk PCT Compatible

PCT ShredderPULSE TubesFT500-S

White No Yes

PCT ShredderPULSE TubesFT500-MS

White Yes Yes

Shredder TubesFT500-PS

Coral No No

Shredder TubesFT500-PMS

Coral Yes No

Features & Benefits of theShredder Tube:

A Variety of Single-Use ProcessingContainers

Standard Tubes for Ambient PressureProcessing

Specialized Tubes for Ultra-High PressureProcessing

Metal Inserts for the Toughest of Samples

Excellent for Collection, Storage,Transport & Processing

Closed Containers to Help Ensure SafetyThroughout the Entire Sample PreparationProcess

Variations and Specifications of Shredder Tubes


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DNA from Spinach Shown on a Pulsed Field Gel

DNA Extraction from spinach using the PBI Shredder System Results in MuchLonger DNA than bead beating methods, while providing comparable yields.

EXTRACTION OF DNA

DNA Extraction from Apple Seeds

Consistent high yields of DNA can beobtained from apple seeds using either

PBI Shredder System.

Shredder Tubes (FT500-PMS) or PCTShredder PULSE Tubes (FT500-MS)with metal inserts give more consistentand higher recovery of DNA from hardsamples, such as apple seeds.

Agarose gel electrophoresis shows thatPBI Shredder Systems yield highermolecular weight genomic DNA, while

the bead beater causes significantshearing of DNA.


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EXTRACTION OF PROTEIN

EXTRACTION OF RNA

RNA Yield

0

0.1

0.2

0.3

0.4

ugRNApermgskmuscle

Shredder

Dounce

High quality RNA suitable for the

preparation of cDNA libraries can beobtained with The PCT ShredderorTheSHREDDER SG3.

Shredder Tubes are compatible with awide variety of sample preparation kitsand reagents including commerciallyavailable chaotropes for the extractionof nucleic acids.

RNA from Rat Skeletal Muscle

Protein from the Nematode C. elegans

Extraction of protein from C. elegansusingThe PCT Shredderand ProteoSolve-CE Native orProteoSolve-CE Stringent Kits from PBI resultsin greater yield of protein than extraction withmortar and pestle with liquid nitrogen (LNP).

PBIs ProteoSolve-CE Native Kit was developedfor the efficient recovery of native proteins andprotein complexes under minimally denaturingconditions in which native conformation andactivity are preserved.


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Protein from Rice GrainsAlthough PBI Shredder Systemsare comparable in yield to mortarand pestle methods, they are farless labor intensive than manualtechniques.

With PBI Shredder Systems thereis no need to transfer the groundmaterial for further processing.

Since Shredder Tubes are single-use disposables, cleaning betweenextractions is no longer necessary.

Protein from Rat Skeletal Muscle Consistent high yields of protein can beobtained from muscle tissue usingPBI Shredder Systems.

Protein yield data demonstrate thatDounce homogenization is lessconsistent (large error bar), while theShredder samples are grouped muchmore tightly (small error bar), indicatinggreater reproducibility.

Note that better results may beobtained for some sample typesusing PCT Shredder PULSE Tubes(FT500-S) or Shredder Tubes (FT500-PS)without metal inserts. This may be dueto the sticky nature of some samples,which can clog the smaller pores of themetal insert.


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Protein from porcine cartilage (~30 mg per sample) extracted inPBI ProteoSolve-IEF Buffer.

Tissue disruption was performed with TheSHREDDER SG3 in PCTShredder PULSE Tubes (FT500-MS) for 40 seconds at lever setting #2or #3.

Comparison samples were disrupted using a commercially availablehomogenizer for 1 minute.

The negative control was generated by soaking a piece of cartilage inPBI ProteoSolve-IEF Buffer without mechanical disruption.

Protein from Porcine Cartilage


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PROTEIN EXTRACTION WITHOUT LIQUID NITROGEN

TUMOR PROTEIN: PBI SHREDDER VS. CRYOGENIC GRINDING

The PBI Shredder System can effectively replace liquid nitrogen grindingin some applications

Cryogenic grinding (mortar and pestle with liquid nitrogen) followedby extraction with PBIs ProteoSolve-TD2 Kit and pressure cyclingtechnology (PCT) yielded less protein than disruption by ThePBI

Shredder System followed by extraction with PBIs ProteoSolve-TD2 Kitand PCT.

ProteoSolve-TD2 Buffer in combination with PCT facilitates recovery ofintact membrane proteins from solid tumors. ProteoSolve-TD2 Buffercontains no organic solvents and is directly compatible with mostsubsequent proteomic analyses (2-D gels, SDS PAGE, liquidchromatography, antibody assays, and mass spectrometry).

ProteoSolve-TD2 Buffer also recovers soluble cytosolic proteins toprovide a more complete representation of the cellular proteome than can

be achieved by other methods.


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EXTRACTION OF MITOCHONDRIA

SPECIAL AND ADVANCED PREPARATIONS

Yield Respiration

PBI Shredder

Glass Homogenizer

Yield and quality of mitochondria prepared from rat skeletal muscle with a PBIShredder System compared to a Teflon/glass homogenizer are similar, but withfar greater ease-of-use, lower cost and safety with the PBI system.

PBI offers specialized kits for the extraction of mitochondria from various tissues.

Mitochondria prepared from freshly harvested tissue using the PBI MITOCHONDRIAISOLATION KIT: RAT MUSCLE are intact, normal and functional, as assessed bywestern blotting, electron microscopy, Ca2+ uptake and respiration assays.

Electron micrograph (4000X) ofmitochondria prepared using a PBIShredder and a PBI MITOCHONDRIAISOLATION KIT: RAT MUSCLE


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PBI Shredder vs. Mortar and Pestle

PREPARING TICKS FOR DETECTION OF TICK-BORNE PATHOGENS

Preparation of ticks for thedetection of tick-borne

pathogens is often done witha razor blade and mortar/pestle.This method is both hazardousand labor intensive.

Recovering the processed tickfrom the mortar requires timeand patience.

PBI Shredder Systems eliminatethe labor and hazard of mortar

and pestle preparation.

Placing Tick inPCT Shredder PULSE Tube

(FT500-MS)

Tick in PCT Shredder PULSETube (FT500-MS)Prior to Shredding

Tick in PCT Shredder PULSETube (FT500-MS)Post-Shredding

Ticks are placed in a PCT Shredder PULSE Tube affixed with a metal insert.

A Shredder Ram is added to the Tube to entrap the tick between the Ram and MetalDisk.

Buffer is then added to the Screw Cap side and the Tube is closed.

The Tube is then placed in either the The PCT Shredderor TheSHREDDER SG3 forprocessing.


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DETECTION OF THE CAUSITIVE AGENT OF LYME DISEASE (Borrelia burgdorferi)

Photographic Insert: A classic presentation of a tick bite showing acharacteristic bulls eye rash, termed Erythema migrans indicatingthe potential for Lyme disease.

Plot: Real-time PCR detection of Borrelia burgdorferi, the causative agentof Lyme disease extracted from the tick that inflicted the bite in the photograph.

Data indicate that this tick harbored B. burgdorferiequivalent to approximately830,000 infectious organisms.


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SHREDDING TREE BARK

Plum Tree Bark Pine Tree Bark

Better shredding of bark is obtained using PCT Shredder PULSE Tubes (FT500-MS) or Shredder Tubes (FT500-PMS) with metal inserts.

PBI Shredder Systems are ideal for processing small samples of bark (


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ADVANTAGES OF THE PBI SHREDDER SYSTEMS

Simple, Easy-To-Use

Inexpensive Hardware and Disposable Sample Containers

Compatible with Routinely Used Lab Buffers and Other ReagentsSingle-Use, Closed, Versatile Processing Container

Self-Contained

No Sample Transfer Required

Reduces the Likelihood of Cross Contamination

Reduces the Chance of Sample Exposure to the User

Reduces the Chance of User Exposure to Potentially HazardousSamples

Excellent for Collection, Storage, Transport, and Processing

Offers Full Chain-of-Custody Tracking from Collection to Test

Available with Metal Inserts for Use with Hard Samples

Such as Seeds

Reduces Sample-to-Sample Variability

Safe No Exposed Sharp Surfaces

Portable Excellent for Field Use


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14 Norfolk Ave, South Easton, MA 02375

TEL 508-230-1828 FAX 508-230-1829www.pressurebiosciences.com

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